-
1
-
-
0027160708
-
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
-
(1993)
Development
, vol.118
, pp. 401-415
-
-
Brand1
Perrimon2
-
2
-
-
0028689568
-
Ectopic expression in Drosophila
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of special interest, A description of the GAL4 system, as compared and contrasted with other techniques for ectopic gene expression. Protocols for targeted gene expression are included.
-
(1994)
Methods Cell Biol
, vol.44
, pp. 635-654
-
-
Brand1
Manoukian2
Perrimon3
-
7
-
-
84910927025
-
An in situ enhancer trap for the detection of cell type specific elements in Drosophila melanogaster
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(1987)
Experientia
, vol.43
, pp. 646
-
-
O'Kane1
-
9
-
-
0023849463
-
GAL4 activates gene expression in mammalian cells
-
(1988)
Cell
, vol.52
, pp. 161-167
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-
Kakidani1
Ptashne2
-
10
-
-
0024299083
-
The yeast UASg is a transcriptional enhancer in human Hela cells in the presence of the GAL4 trans-activator
-
(1988)
Cell
, vol.52
, pp. 169-178
-
-
Webster1
Jin2
Green3
Hollis4
Chambon5
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14
-
-
0029011452
-
Distribution, classification, and development of Drosophila glial cells in the late embryonic and early larval ventral nerve cord
-
of outstanding interest, This paper describes an enhancer detection screen for lines that express GAL4 in different types of glial cells. The GAL4 lines were used to drive expression of UAS-lacZ and UAS-kinesin-lacZ. The authors present a comprehensive description of glia in the embryonic central nervous system, including a three dimensional reconstruction.
-
(1995)
Rouxs Arch Dev Biol
, vol.204
, pp. 284-307
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-
Ito1
Urban2
Technau3
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16
-
-
0028918248
-
Genetic feminization of brain structures and changed sexual orientation in male Drosophila
-
of outstanding interest, The authors use the GAL4 system to target expression of genes in the Drosophila brain. GAL4-controlled ectopic expression of the female form of the transformer gene in the antennal lobes or mushroom bodies of male flies leads to bisexual behaviour.
-
(1995)
Science
, vol.267
, pp. 902-905
-
-
Ferveur1
Stortkuhl2
Stocker3
Greenspan4
-
17
-
-
0029089595
-
Subdivision of the Drosophila mushroom bodies by enhancer-trap expression patterns
-
of outstanding interest, The CAL4 system was used to label neurons in the brain and to identify subsets of cells in the mushroom bodies.
-
(1995)
Neuron
, vol.15
, pp. 45-54
-
-
Yang1
Armstrong2
Villinsky3
Strausfeld4
Kaiser5
-
18
-
-
23444431611
-
Green fluorescent protein as a marker for gene expression
-
of outstanding interest, This paper describes the first use of GFP as a marker in living animals. GFP was used to mark specific neurons in the nematode Caenorhabditis elegans.
-
(1994)
Science
, vol.263
, pp. 802-805
-
-
Chalfie1
Tu2
Euskirchen3
Ward4
Prasher5
-
19
-
-
0028227833
-
Implications for Bcd messenger RNA localization from spatial distribution of exu protein in Drosophila oogenesis
-
(1994)
Nature
, vol.369
, pp. 400-403
-
-
Wang1
Hazelrigg2
-
20
-
-
0029166758
-
GFP in Drosophila
-
of outstanding interest, The GAL4 system can be used to express the naturally fluorescent green fluorescent protein from the jellyfish, Aequoria victoria. This is particularly exciting as it enables visualization of cells in the living organism. Nervous system development can thus be followed in vivo. By fusing GFP to the microtubule-binding protein tau, microtubules can be followed in vivo. Tau—GFP is an excellent marker for axon morphology.
-
(1995)
Trends Genet
, vol.11
, pp. 324-325
-
-
Brand1
-
21
-
-
0029118583
-
Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila
-
of outstanding interest, A GAL4-responsive GFP transgene was constructed to label living cells at all stages of Drosophila development. GFP expression was monitored in living embryos and larvae, demonstrating the potential of GFP for visualizing cells throughout development.
-
(1995)
Proc Natl Acad Sci USA
, vol.92
, pp. 7036-7040
-
-
Yeh1
Gustafson2
Boulianne3
-
22
-
-
0027518831
-
The Drosophila gene pointed encodes two Ets like proteins, which are involved in the development of the midline glial cells
-
(1993)
Development
, vol.117
, pp. 163-176
-
-
Klambt1
-
23
-
-
0028206040
-
The Ets transcription factors encoded by the Drosophila gene pointed direct glial cell differentiation in the embryonic CNS
-
of outstanding interest, The GAL4 system is used to investigate the role of a gene involved in cell fate determination in the nervous system. Ectopic pointed expression promotes a glial cell fate. The experiments suggest that glial-neuronal interactions can induce the expression of neuronal markers.
-
(1994)
Cell
, vol.78
, pp. 149-160
-
-
Klaes1
Menne2
Stollewerk3
Scholz4
Klambt5
-
27
-
-
0028944665
-
Induction of ectopic eyes by targeted expression of the eyeless gene in Drosophila
-
of outstanding interest, In this fascinating study, the role of the eyeless gene in controlling development of a complete eye is shown. GAL4-driven ectopic expression of eyeless in imaginal discs leads to ectopic eyes on the antennae, legs and halteres. Expression of the mouse small eye gene in Drosophila can also induce eye formation.
-
(1995)
Science
, vol.267
, pp. 1788-1792
-
-
Halder1
Callaerts2
Gehring3
-
29
-
-
0027351577
-
Developmental mechanisms that generate precise patterns of neuronal connectivity
-
(1993)
Cell
, vol.72
, pp. 77-98
-
-
Goodman1
Shatz2
-
32
-
-
0023652392
-
Expression of fasciclin I and II glycoproteins on subsets of axon pathways during neuronal development in the grasshopper
-
(1987)
Cell
, vol.48
, pp. 745-755
-
-
Bastiani1
Harrelson2
Snow3
Goodman4
-
33
-
-
0026004415
-
Genetic analysis of growth cone guidance in Drosophila: fasciclin II functions as a neuronal recognition molecule
-
(1991)
Cell
, vol.67
, pp. 45-57
-
-
Grenningloh1
Rehm2
Goodman3
-
35
-
-
0027959402
-
Genetic analysis of fasciclin II in Drosophila defasciculation, refasciculation, and altered fasciculation
-
of outstanding interest, GAL4-mediated extopic expression of FasII in the CNS can rescue the FasII loss-of-function phenotype. Increased levels of FasII generate a gain-of-function phenotype characterized by excessive fasciculation.
-
(1994)
Neuron
, vol.13
, pp. 1055-1069
-
-
Lin1
Fetter2
Kopczynski3
Grenningloh4
Goodman5
-
36
-
-
0028147879
-
Ectopic and increased expression of fasciclin II alters motoneuron growth cone guidance
-
of outstanding interest, GAL4-driven FasII expression on motor neurons interferes with axon pathfinding by preventing axon defasciculation at specific choice points. As a result, motor neurons often bypass their targets.
-
(1994)
Neuron
, vol.13
, pp. 507-523
-
-
Lin1
Goodman2
-
37
-
-
0028211876
-
Raf acts downstream of the EGF receptor to determine dorsoventral polarity during Drosophila oogenesis
-
(1994)
Genes Dev
, vol.8
, pp. 629-639
-
-
Brand1
Perrimon2
-
38
-
-
0027984806
-
The Ets domain protein pointed P2 is a target of MAP kinase in the sevenless signal transduction pathway
-
of special interest, A sevenless—GAL4 line is used to control PntP2 expression in the eye, so as to study its role in signal transduction during eye development.
-
(1994)
Nature
, vol.370
, pp. 386-389
-
-
Brunner1
Ducker2
Oellers3
Hafen4
Scholz5
Klambt6
-
39
-
-
0028086441
-
Distinct morphogenetic functions of similar small GTPases: Drosophila Drac1 is involved in axonal outgrowth and myoblast fusion
-
of special interest, GAL4-controlled expression of constitutively active and dominant-negative Drac1 mutants was used to study the role of Drac1 in peripheral axon outgrowth and myoblast fusion.
-
(1994)
Genes Dev
, vol.8
, pp. 1787-1802
-
-
Luo1
Liao2
Jan3
Jan4
-
40
-
-
0029019928
-
Yan functions as a general inhibitor of differentiation and is negatively regulated by activation of the Ras1/MAPK pathway
-
of special interest, A constitutively active form of yan ectopically expressed under the control of GAL4 can prevent nervous system differentiation.
-
(1995)
Cell
, vol.81
, pp. 857-866
-
-
Rebay1
Rubin2
-
41
-
-
0028803421
-
Targeted ablation of glia disrupts axon tract formation in the Drosophila CNS
-
of outstanding interest, The GAL4 system is used to express the toxin ricin as a means of targeted cell ablation to investigate the role of the interface glia in the establishment of axon tracts. Ablation of the interface glia results in the loss of the longitudinal connectives, demonstrating an essential role for glial cells in axon pathfinding.
-
(1995)
Development
, vol.121
, pp. 3703-3712
-
-
Hidalgo1
Urban2
Brand3
-
42
-
-
0022414029
-
Early events in insect neurogenesis II
-
(1985)
Dev Biol
, vol.111
, pp. 206-219
-
-
Doe1
Goodman2
-
46
-
-
0020617373
-
The development of substance P, somatostatin and vasoactive intestinal polypeptide in sympathetic and spinal sensory ganglia of the chick embryo
-
(1983)
Neuroscience
, vol.10
, pp. 31-41
-
-
Raper1
Bastiani2
Goodman3
-
47
-
-
0024396624
-
Transient pioneer neurons are essential for formation of an embryonic peripheral nerve
-
(1989)
Science
, vol.245
, pp. 982-984
-
-
Klose1
Bentley2
-
48
-
-
0028913607
-
Targeted neuronal cell ablation in the Drosophila embryo: pathfinding by follower growth cones in the absence of pioneers
-
of outstanding interest, This paper described the use of the GAL4 system for targeted cell ablation, using diphtheria toxin. The method generates mosaic patterns of toxin expression. The results suggest that pioneer neurons are not essential for axon tract formation.
-
(1995)
Neuron
, vol.14
, pp. 707-715
-
-
Lin1
Auld2
Goodman3
-
49
-
-
0028815501
-
Targeted expression of tetanus toxin light chain in Drosophila specifically eliminates synaptic transmission and causes behavioral defects
-
of outstanding interest, Targeted expression of tetanus toxin light chain can be used to prevent synaptic transmission in vivo. This provides a potential tool for the future analysis of behaviour. In this paper, for example, the olfactory escape response is assayed in flies expressing tetanus toxin.
-
(1995)
Neuron
, vol.14
, pp. 341-351
-
-
Sweeney1
Broadie2
Keane3
Niemann4
O'Kane5
-
53
-
-
0028297332
-
Associative odour learning in Drosophila is abolished by chemical ablation of the mushroom bodies
-
(1994)
Science
, vol.263
, pp. 692-695
-
-
De Belle1
Heisenberg2
-
54
-
-
0029084473
-
Functional dissection of the Drosophila mushroom bodies by selective feminization of genetically defined subcompartments
-
of outstanding interest, The GAL4 system is used to show that different subsets of Kenyon cells in the mushroom bodies may have different functions in behaviour. The female form of the transformer gene is misexpressed in subsets of these cells to investigate effects on male courtship behaviour.
-
(1995)
Neuron
, vol.15
, pp. 55-61
-
-
O'Dell1
Armstrong2
Yang3
Kaiser4
-
55
-
-
0028173714
-
Tau beta-galactosidase, an axon targeted fusion protein
-
of special interest, The reported β-galactosidase is fused to the microtubule-binding protein, tau. Tau—β-galactosidase highlights the cytoskeleton and clearly reveals neuronal and glial cell morphology.
-
(1994)
Proc Natl Acad Sci USA
, vol.91
, pp. 5972-5976
-
-
Callahan1
Thomas2
-
56
-
-
0028944522
-
Mammalian CD2 Is an Effective Heterologous Marker of the Cell Surface in Drosophila
-
of special interest, The transmembrane protein CD2 is used as a GAL4-responsive cell surface marker.
-
(1995)
Developmental Biology
, vol.168
, pp. 689-693
-
-
Dunin Borkowski1
Brown2
-
57
-
-
0027323658
-
Asense is a Drosophila neural precursor gene and is capable of initiating sense organ formation
-
(1993)
Development
, vol.119
, pp. 1-17
-
-
Brand1
Jarman2
Jan3
Jan4
-
58
-
-
0028178791
-
The basic helix loop helix domain of Drosophila lethal of scute protein is sufficient for proneural function and activates neurogenic genes
-
of special interest, Lethal of scute is usually dispensable for adult sense organ development. However, ectopic expression of the gene demonstrates that it is able to promote sense organ development independently of the other proneural genes, revealing functional redundancy in the development of the nervous system. The basic helix-loop-helix domain of the lethal of scute protein is sufficient to promote sense organ development.
-
(1994)
Cell
, vol.76
, pp. 77-87
-
-
Hinz1
Giebel2
Campos-Ortega3
-
59
-
-
0029017531
-
Inhibition of cell fate in Drosophila by Enhancer of split genes
-
of special interest, The authors demonstrate that ectopic expression of Enhancer of split can inhibit cells from acquiring a neuronal cell fate.
-
(1995)
Mech Dev
, vol.51
, pp. 305-315
-
-
Tata1
Hartley2
-
60
-
-
0028905887
-
Serrate expression can functionally replace Delta activity during neuroblast segregation in the Drosophila embryo
-
of special interest, The GAL4 system was used to express Serrate ectopically to determine whether Serrate could mediate similar functions to the neurogenic gene and Notch ligand, Delta. In this study, Serrate is shown to be capable of replacing the Delta gene during development of the nervous system, leading to a suppression of proneural genes such as achaete in the proneural clusters. Thus, Serrate may encode an alternative ligand for Notch.
-
(1995)
Development
, vol.121
, pp. 855-865
-
-
Gu1
Hukriede2
Fleming3
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