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Bioorganic and Medicinal Chemistry Letters
Volumn 5, Issue 22, 1995, Pages 2755-2760
Inhibition of an HIV-1 Tat-derived peptide binding to TAR RNA by aminoglycoside antibiotics
Author keywords

[No Author keywords available]
Indexed keywords

AMINOGLYCOSIDE ANTIBIOTIC AGENT; FRAMYCETIN; GENTAMICIN; STREPTOMYCIN; TRANSACTIVATOR PROTEIN;
EID: 0028823853     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0960-894X(95)00467-8     Document Type: Article
Times cited : (150)
References (30)
  • 15
    • 84916558515 scopus 로고    scopus 로고
    • 12: calculated, 1616.9; found, 1617.6).
  • 16
    • 84916558514 scopus 로고    scopus 로고
    • 32P pCp (Amersham) and reannealed by heating to 90°C before use for binding studies. All binding studies were performed in a buffer solution containing 10 mM Tris-HCl (pH 7.5), 70 mM NaCl, 0.2 mM EDTA, 0.01% Nonidet P-40 (Sigma), 5% glycerol. The RNA mobility-shift assays were performed on 1.5-mm thick, non-denaturing gels (15% or 20% acrylamide with 1/75 of bis).
  • 17
    • 0026589545 scopus 로고
    • i, for L-arginine was 4 mM, fot L-argininamide was 1 mM in inhibiting a Tat peptide (residues 49 to 57) binding to TAR RNA.
    • (1992) Proc. Natl. Acad. Sci. USA , vol.89 , pp. 2723
    • Tao1    Frankel2
  • 18
    • 84916558513 scopus 로고    scopus 로고
    • 7
  • 19
    • 84916558512 scopus 로고    scopus 로고
    • 12 binding.
  • 20
    • 84916558511 scopus 로고    scopus 로고
    • 50 for each aminoglvcoside were performed.
  • 21
    • 84916558510 scopus 로고    scopus 로고
    • 31 was observed.
  • 22
    • 0020708497 scopus 로고
    • Under pH 7.5 buffered conditions, neomycin is reasonably assumed to have a net +6 charge, gentamicin should have a +5 charge, while streptomycin should carry a +3 charge.
    • (1983) J. Amer. Chem. Soc. , vol.105 , pp. 1021
    • Botto1    Coxon2
  • 23
    • 84916558509 scopus 로고    scopus 로고
    • 31.
  • 24
    • 84916558508 scopus 로고    scopus 로고
    • 31. The molecular details of these antibiotics' interactions with TAR RNA are the subject of current investigation.
  • 25
    • 84916558507 scopus 로고    scopus 로고
    • Based on the same observation, this also suggests that caution should be taken in interpretating the gel mobility shift data of Tat/TAR inhibition by aminoglycosides.
  • 26
    • 84916558506 scopus 로고    scopus 로고
    • d in μM range). The mean of the values obtained with their standard deviations for neomycin, streptomycin and gentamicin were 0.7±0.2, 2.4±0.2, and 4.3±0.9 μM, respectively.
  • 27
    • 84916558505 scopus 로고    scopus 로고
    • 50 values. Compared with footpringing-type experiments which were usually performed at ≈ 0.1 mM concentrations of aminoglycoside, these data provide strong circumstantial evidence that the antibiotics block Tat peptide from binding to TAR RNA by interacting with TAR RNA.
  • 28
    • 84916558504 scopus 로고    scopus 로고
    • 23
  • 29
    • 84916558503 scopus 로고    scopus 로고
    • When neomycin was added greater than 1 μM, the reduction of the first neomycin/TAR complex was accompanied by increasing amounts of both the higher-order complex and free TAR. Further investigation is underway to understand the nature of these antibiotic/TAR complexes.

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.