Direct Random Mutagenesis of Gene-Sized DNA Fragments Using Polymerase Chain Reaction

Analytical Biochemistry

Volumn 224, Issue 1, 1995, Pages 347-353

  Fromant, Michel ^a   Blanquet, Sylvain ^a   Plateau, Pierre ^a  

^a LABORATOIRE DE BIOCHIMIE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; MANGANESE COMPOUNDS; MUTAGENESIS;

DNA FRAGMENT; GC AND GC; MUTAGENESIS EXPERIMENT; RANDOM MUTAGENESIS;

POLYMERASE CHAIN REACTION;

ARTICLE; DNA DETERMINATION; GENE AMPLIFICATION; MUTAGENESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 0028816556     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1006/abio.1995.1050     Document Type: Article

References (30)

1. Zoller, M. J. (1991) Curr. Opin. Biotechnol. 2, 526-531.
2. Hutchison, C. A., Nordeen, S. K., Vogt, K., and Edged, M. H. (1986) Proc. Natl. Acad. Sci. USA 83, 710-714.
3. Shortle, D., and Botstein, D. (1983) Methods Enzymol. 100, 457-468.
4. Myers, R. M., Lerman, L. S., and Maniatis, T. (1985) Science 229, 242-247.
5. Busby, S., Irani, M., and de Crombrugghe, B. (1982) J. Mol. Biol. 154, 197-209.
6. Grompe, M., Muzny, D. M., and Caskey, C. T. (1989) Proc. Natl. Acad. Sci. USA 86, 5888-5892.
7. Montandon, A. J., Green, P. M., Giannedi, F., and Bentley, D. R. (1989) Nucleic Acids Res. 17, 3347-3358.
8. Kadonaga, J. T., and Knowles, J. R. (1985) Nucleic Acids Res. 13, 1733-1745.
9. Lehtovaara, P. M., Koivula, A. K., Bamford, J., and Knowles, J. K. C. (1988) Protein Eng. 2, 63-68.
10. Tindall, K. R., and Kunkel, T. A. (1988) Biochemistry 27, 6008-6013.
11. Eckert, K. A., and Kunkel, T. A. (1990) Nucleic Acids Res. 18, 3739-3744.
12. Bloch, W. (1991) Biochemistry 30, 2735-2747.
13. Keohavong, P., and Thilly, W. G. (1989) Proc. Natl. Acad. Sci. USA 86, 9253-9257.
14. Leung, D. W., Chen, E., and Goeddel, D. V. (1989) Technique 1, 11-15.
15. Liao, X., and Wise, J. A. (1990) Gene 88, 107-111.
16. Cadwell, R. C., and Joyce, G. F. (1992) PCR Methods Appl. 2, 28-33.
17. Zhou, Y., Zhang, X., and Ebright, R. H. (1991) Nucleic Acids Res. 19, 6052.
18. Leveque, F., Plateau, P., Dessen, P., and Blanquet, S. (1990) Nucleic Acids Res. 18, 305-312.
19. Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Gene 33, 103-119.
20. Hirel, P. H., Leveque, F., Mellot, P., Dardel, F., Panvert, M., Mechulam, Y., and Fayat, G. (1988) Biochimie 70, 773-782.
21. Sanger, F., Nicklen, S., andCoulson, A. R. (1977) Proc. Natl. Acad. Sci. USA 74, 5463-5467.
22. Dunning, A. M., Talmud, P., and Humphries, S. E. (1988) Nucleic Acids Res. 16, 10393.
23. Echols, H. (1991) Annu. Rev. Biochem. 60, 477-511.
24. Fersht, A. (1985) Enzyme Structure and Mechanism, pp. 112, Freeman, New York.
25. Mendelman, L. V., Boosalis, M. S., Petruska, J., and Goodman, M. F. (1989) J. Biol. Chem. 264, 14415-14423.
26. Kwok, S., Kellogg, D. E., McKinney, N., Spasic, D., Goda, L., Lev-enson, C., and Sninsky, J. J. (1990) Nucleic Acids Res. 18, 999-1005.
27. Eckert, K. A., and Kunkel, T. A. (1992) in PCR: A Practical Approach (McPherson, M. J., Quirke, P., and Taylor, G. R., Eds.), pp. 225-244, Oxford Univ. Press, New York.
28. Krawczak, M-, Reiss, J., Schmidtke, J., and Rosier, U. (1989) Nucleic Acids Res. 17, 2197-2201.
29. Eckert, K. A., and Kunkel, T. A. (1991) PCR Methods Appl. 1, 17-24.
30. Huang, M. M., Arnheim, N., and Goodman, M. F. (1992) Nucleic Acids Res. 20, 4567-4573.