Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps

Cell

Volumn 83, Issue 3, 1995, Pages 387-395

  McBlane, J Fraser ^a   van Gent, Dik C ^a   Ramsden, Dale A ^a   Romeo, Charles ^c   Cuomo, Christina A ^b,c   Gellert, Martin ^a   Oettinger, Marjorie A ^b,c  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

T LYMPHOCYTE RECEPTOR;

ARTICLE; GENE EXPRESSION; GENE SEQUENCE; GENETIC RECOMBINATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION;

EID: 0028805853     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/0092-8674(95)90116-7     Document Type: Article

References (32)

1. VDJ recombination
2. An active v-abl protein tyrosine kinase blocks immunoglobulin light-chain gene rearrangement
3. The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system
4. Transposable elements generate novel spatial patterns of gene expression in Antirrhinum majus
5. The mechanism of conservative site-specific recombination
6. Analysis of regions of RAG-2 important for V(D)J recombination
7. HIV-1 DNA integration: mechanism of viral DNA cleavage and DNA strand transfer
8. Molecular analysis of V(D)J recombination
9. V(D)J recombination: a functional definition of the joining signals
10. A short polypeptide marker sequence useful for recombinant protein identification and purification
11. A V(D)J site-specific recombination model involving no compulsory double-stranded break formation at the coding segments
12. The mechanism of V(D)J joining: lessons from molecular, immunological, and comparative analyses
13. Inversion of the phosphate chirality at the target site of Mu DNA strand transfer: evidence for a one-step transesterification mechanism
14. RAG-1-deficient mice have no mature B and T lymphocytes
15. New mammalian expression vectors
16. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination
17. Formation and resolution of double strand break intermediates in V(D)J rearrangement
18. V(D)J recombination in mouse thymocytes: double-strand breaks near T cell receptor δ rearrangement signals
19. V(D)J recombination: broken DNA molecules with covalently sealed (hairpin) coding ends in scid mouse thymocytes
20. Characterization of broken DNA molecules associated with V(D)J recombination
21. Expression and V(D)J recombination activity of mutated RAG-1 proteins
22. Definition of a core region of RAG-2 that is functional in V(D)J recombination
23. RAG-1 mutations that affect the target specificity of V(D)J recombination: a possible direct role of RAG-1 in site recognition
24. The V(D)J recombination activating gene, RAG-1
25. Double-strand signal sequence breaks in V(D)J recombination are blunt, 5′-phosphorylated, RAG-dependent, and cell-cycleregulated
26. RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
27. Dispensable sequence motifs in the RAG-1 and RAG-2 genes for plasmid V(D)J recombination
28. Initiation of V(D)J recombination in a cellfree system
29. Characterization of coding ends in thymocytes of scid mice: implications for the mechanism of V(D)J recombination