Expression of functional growth hormone receptors in cultured human osteoblast-like cells

Journal of Clinical Endocrinology and Metabolism

Volumn 80, Issue 12, 1995, Pages 3483-3488

  Nilsson, Anders ^a   Swolin, D ^a   Enerback, S ^a   Ohlsson, C ^a  

^a SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

GROWTH HORMONE; GROWTH HORMONE RECEPTOR;

ARTICLE; BINDING SITE; BONE DENSITY; BONE REMODELING; GENE EXPRESSION; HUMAN; HUMAN CELL; NORTHERN BLOTTING; OSTEOBLAST; PRIORITY JOURNAL;

BASE SEQUENCE; CELLS, CULTURED; GROWTH HORMONE; HUMAN; MOLECULAR PROBES; MOLECULAR SEQUENCE DATA; OSTEOBLASTS; RECEPTORS, SOMATOTROPIN; RNA, MESSENGER; SUPPORT, NON-U.S. GOV'T; THYMIDINE;

EID: 0028791174     PISSN: 0021972X     EISSN: 19457197     Source Type: Journal    
DOI: 10.1210/jcem.80.12.8530587     Document Type: Article

References (47)

1. Isaksson OGP, JanssonJ-O, Gause IAM. 1982 Growth hormone stimulates longitudinal bone growth directly. Science. 216:1237-1239.
2. Nilsson A, Isgaard J, Lindahl A, Peterson L, Isaksson O. 1987 Effects of unilateral arterial infusion of GH and IGF-I on tibial longitudinal bone growth in hypophysectomized rats. Calcif Tissue Int. 40:91-96.
3. Ohlsson C, Nilsson A, Isaksson O, Lindahl A. 1992 Growth hormone induces muliplication of the slowly cycling germinal cells of the rat tibial growth plate. Proc Natl Acad Sci USA. 89:9826-9830.
4. Harris WH, Heaney RP. 1969 Effect of growth hormone on skeletal mass in adult dogs. Nature. 223:403-404.
5. Inzucchi SE, Robbins RJ. 1994 Effects of growth hormone on human bone biology. J Clin Endocrin Metab. 79:691-694.
6. Hyer SL, Rodin DA, Tobias JH, Leiper A, Nussey SS. 1992 Growth hormone deficiency during puberty reduces adult bone mineral density. Arch Dis Child. 67:1472-1474.
7. RosénT, Hansson T, Granhed H, Szucs J, BengtssonB-Å. 1993 Reduced bone mineral content in adult patients with growth hormone deficiency. Acta Endocrinol (Copenh). 129:201-206.
8. Nielsen HK, JörgensenJOL, Brixen K, Christiansen JS. 1991 Serum osteocalcin and bone isoenzyme alkaline phosphatase in growth hormone-deficient patients: dose-response studies with biosynthetic human GH. Calcif Tissue Int. 48:82-87.
9. Saggese G, Baroncelli GI, Bertelloni S, Cinquanta L, Di Nero G. 1993 Effects of long-term treatment with growth hormone on bone and mineral metabolism in children with growth hormone deficiency. J Pediatr. 122:37-45.
10. RosénT, Johansson G, Hallgren P, Caidahl K, Bosaeus I, BengtssonB-Å. 1994 Beneficial effects of 12 months replacement therapy with recombinant human growth hormone to growth hormone deficient adults. Endocrinol Metab Clin North Am. 1:55-66.
11. Diamond T, Nery L, Posen S. 1989 Spinal and peripheral bone mineral densities in acromegaly: the effects of excess growth hormone and hypogonadism. Ann Intern Med. 111:567-573.
12. Morel G, Chavassieux P, Barenton B, Dubois PM, Meunier PJ, Boivin G. 1993 Evidence for a direct effect of growth hormone on osteoblasts. Cell Tissue Res. 273:279-286.
13. Lobie PE, Garcia-Aragon J, Wang BS, Baumbach WR, Waters MJ. 1992 Cellular localization of the growth hormone binding protein in the rat. Endocrinology. 130:3057-3065.
14. Barnard R, Ng KW, Martin TJ, Waters MJ. 1991 Growth hormone (GH) receptors in clonal osteoblast-like cells mediate a mitogenic response to GH. Endocrinology. 128:1459-1464.
15. Slootweg MC, van Buul-Offers SC, Herrmann-Erlee MPM, Duursma SA. 1988 Direct stimulatory effect of growth hormone on DNA synthesis of fetal chicken osteoblasts in culture. Acta Endocrinol (Copenh). 18:294-299.
16. Slootweg MC, van Buul-Offers SC, Herrmann-Erlee MPM, van der Meer JM, Duursma SA. 1988 Growth hormone is mitogenic for fetal mouse osteoblasts but not for undifferentiated bone cells. J Endocrinol. 116:R11-R13.
17. Ernst M, Froesch ER. 1988 Growth hormone dependent stimulation of osteoblast-like cells in serum-free cultures via local synthesis of insulin-like growth factor I. Biochem Biophys Res Comm. 151:142-147.
18. Scheven BA, Hamilton NJ, Fakkeldij TM, Duursma SA. 1991 Effects of recombinant human insulin-like growth factor I and II (IGF-I/-II) and growth hormone (GH) on the growth of normal adult human osteoblast-like cells and human osteogenic sarcoma cells. Growth Regulation. 1:160-167.
19. Kassern M, Blum W, Ristelli J, Mosekilde L, Eriksen EF. 1993 Growth hormone stimulates proliferation and differentiation of normal human osteoblast-like cells in vitro. Calcif Tissue Int. 52:222-226.
20. Kassem M, Mosekilde L, Eriksen EF. 1994 Growth hormone stimulates proliferation of normal human bone marrow stromal osteoblast precursor cells in vitro. Growth Regulation. 4:131-135.
21. 3 on the production of prostaglandins by cells derived from human bone. FEBS Lett. 169:49-52.
22. Crisp AJ, McGuire-Goldring MB, Goldring SR. 1984 A system for culture of human trabecular bone and hormone response profiles of derived cells. Br J Exp Pathol. 65:645-654.
23. Moss DW. 1966 A note on the spectrophotometric estimation of alkaline phosphatase activity. Enzymologia. 31:193-202.
24. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951 Protein measurement with the Folin phenol reagent. J Biol Chem. 193:265-275.
25. Salacinski PRP, McLean C, Sykes JE, Clement-Jones VV, Lowry PJ. 1981 Iodidination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3a,6a-diphenyl gly-coluril (Iodogen). Anal Biochem. 117:136-146.
26. Nilsson A, Lindahl A, Ede'nS, Isaksson OGP. 1989 Demonstration of growth hormone receptors in cultured rat epiphyseal chondrocytes by specific binding of growth hormone and immunohisto-chemistry. J Endocrinol. 122:69-77.
27. Scatchar dG. 1949 The attraction of proteins for small molecules and ions. Ann NY Acad Sci. 51:660-672.
28. Urbanek M, MacLeod JN, Cooke NE, Liebhaber SA. 1992 Expression of a human growth hormone (hGH) receptor isoform is predicted by tissue-specific alternative splidng of exon 3 of the hGH receptor gene transcript. Mol Endocrinol. 6:279-287.
29. Dumam DM, Palmiter RD. 1983 A practical approach for quantitating specific mRNAs by solution hybridization. Anal Biochem. 131:385-393.
30. Mathews LS, Norstedt G, Palmiter RD. 1986 Regulation of insulinlike growth factor I gene expression by growth hormone. Proc Natl Acad Sci USA. 83:9343-9347.
31. Labarca C, Paigen K. 1980 A simple, rapid, and sensitive DNA assay procedure. Anal Biochem. 102:344-352.
32. Chomczynski P, Sacchi N. 1987 Single-step method of RNA isolation by acid guanidinium thiocyanat-phenol-chloroform extraction. Anal Biochem. 162:156-159.
33. Lewison D, Shenzer P, Hochberg Z. 1993 Growth hormone involvement in the regulation of tartrate-resistant acid phosphatase-positive cells that are active in cartilage and bone resorption, Calcif Tissue Int. 52:216-221.
34. Leung DW, Spencer SA, Cachianes G, et al. 1987 Growth hormone receptor and serum binding protein; purification, cloning and expression. Nature. 330:537-543.
35. Deiehaye-Zervas MC, Mertani H, Martani JF, Nihoul-Fekete'C, Morel G, Postel-Vinay MC. 1994 Expression of the growth hormone receptor gene in human digestive tissues. J Clin Endocrinol Metab. 78:1473-1480.
36. Carlsson B, Ede'nS, Nilsson A, et al. 1991 Expression and physiological significance of growth hormone receptors and growth hormone binding proteins in rat and man. Acta Paediatr Scand. [Suppl] 379:70-76.
37. Ohlsson C, Nilsson A, Isaksson OGP, Lindahl A. 1992 Effect of growth hormone and insulin-like growth factor I on DNA synthesis and matrix production in rat epiphyseal chondrocytes in monolayer culture. J Endocrinol. 133:291-300.
38. Bagi CM, Brommage R, Deleon L, Adams S, RosénD, Sommer A. 1994 Benefit of systemically administered rhIGF-1 and rhIGF-I/IGFBP-3 on cancellous bone in ovariectomized rats. J Bone Miner Res. 9:1301-1312.
39. Narusawa K, Nakamura T, Matsuoka Y, Lee L, Tanaka H, Seino Y. 1993 IGF-I/IGFBP-3 complex administration stimulates bone formation in rats. J Bone Miner Res. [Suppl 1]8:272(Abstract).
40. Johansson AG, Lindh E, Ljunghall S. 1992 Insulin-like growth factor I stimulates bone turnover in osteoporosis. Lancet. 339:1619.
41. 3 and activity on human osteoblast-like cells in short term cultures. Bone. 11:81-86.
42. StrömP, Ljunghall S, Melhus H. 1994 Adult human osteoblast-like cells do not express insulin-like growth factor-I. Biochem Biophys Res Comm. 199:78-82.
43. Mohan S, Baylink DJ. 1991 Bone growth factors. Clin Orthop. 263: 30-48.
44. Raile K, HöflichA, Kessler U, et al. 1994 Human osteosarcoma (U-2 OS) cells express both insuline-like growth factor I (IGF-I) receptors and insulin-like growth factor-II/mannose-6-phosphate (IGF-II/ M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J Cell Physiol. 159:531-541.
45. Gallagher JA, Beresford JN, McGuire MKB, et al. 1984 Effects of Glucocorticoids and anabolic steroids on cells derived from human skeletal and articular tissue in vitro. In: Avioli LV, Gennari C, Imbimbo B, (eds) Advances in Experimental Medicine and Biology. New York, Plenum Press, vol 171:279-291.
46. Wong MM, Rao LG, Ly H, et al. 1990 Long-term effects of physiological concentrations of dexamethasone on human bone-derived cells. J Bone Miner Res. 5:803-813.
47. Subramaniam M, Colvard D, Keeting PE, Rasmussen K, Riggs BL, Spelsberg TC. 1992 Glucocorticoid regulation of alkaline phosphatase, osteocalcin, and proto-oncogenes, in normal human osteoblast-like cells. J Cell Biochem. 50:411-424.