Analysis of the B-cell progenitor compartment at the level of single cells

Current Biology

Volumn 4, Issue 7, 1994, Pages 573-583

  Ehlich, Andreas ^a   Martin, Verena ^a   Müller, Werner ^a   Rajewsky, Klaus ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN J CHAIN; IMMUNOGLOBULINS, DELTA CHAIN; IMMUNOGLOBULINS, HEAVY CHAIN; IMMUNOGLOBULINS, J CHAIN;

ANIMAL; ARTICLE; B LYMPHOCYTE; BIOLOGICAL MODEL; CYTOLOGY; GENE REARRANGEMENT; GENETICS; HEMATOPOIETIC STEM CELL; IMMUNOGLOBULIN GENE; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR GENETICS; MOUSE; MOUSE STRAIN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

ANIMAL; B-LYMPHOCYTES; BASE SEQUENCE; GENE REARRANGEMENT, B-LYMPHOCYTE, HEAVY CHAIN; GENES, IMMUNOGLOBULIN; HEMATOPOIETIC STEM CELLS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOGLOBULINS, DELTA-CHAIN; IMMUNOGLOBULINS, HEAVY-CHAIN; IMMUNOGLOBULINS, J-CHAIN; MICE; MICE, INBRED BALB C; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SUPPORT, NON-U.S. GOV'T;

ANIMALIA;

EID: 0028466384     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(00)00129-9     Document Type: Article

References (59)

1. Somatic generation of antibody diversity
2. Development of the primary antibody repertoire
3. Only DFL16, DSP2, DQ52 gene families exist in mouse immunoglobulin heavy chain diversity gene loci, of which DFL16 and DSP2 originate from the same primordial DH gene
4. Novel immunoglobulin heavy chains are produced from DJH gene segment rearrangements in lymphoid cells
5. H element reading frames
6. Organization and reorganization of immunoglobulin genes in A-MuLV-transformed cells: rearrangement of heavy but not light chain genes
7. Allelic exclusion in transgenic mice that express the membrane form of immunoglobulin μ
8. Feedback inhibition of immunoglobulin gene rearrangement by membrane mu, but not by secreted mu heavy chains [published erratum appears in J Exp Med 1989 Jun 1;169(6):2269]
9. Targeted disruption of μ chain membrane exon causes loss of heavy-chain allelic exclusion
10. Isolation of scid pre-B cells that rearrange kappa light chain genes: formation of normal signal and abnormal coding joins
11. Activation of immunoglobulin kappa gene rearrangement correlates with induction of germline kappa gene transcription
12. Light-chain gene expression before heavy-chain gene rearrangement in pre-B cells transformed by Epstein–Barr virus
13. Immunoglobulin heavy and light chain genes rearrange independently at early stages of B cell development
14. H locus
15. Rearrangement and expression of κ light chain genes can occur without μ heavy chain expression during differentiation of pre-B cells
16. k gene rearrangement in pre-B cells follows the expression of membranebound immunoglobulin heavy chains
17. Molecular requirements for the μ-induced light chain gene rearrangement in pre-B cells
18. Crosslinking of the cell surface immunoglobulin (μ-surrogate light chains complex) on pre-B cells induces activation of V gene rearrangements at the immunoglobulin κ locus
19. Normal pre-B cells express a receptor complex of μ heavy chains and surrogate light-chain proteins
20. The expression of the IgH chain repertoire in developing bone marrow B lineage cells
21. H81X gene segments evidences a dependence of B cell clonal maturation on the structure of nascent H chains
22. The products of the pre-B cell-specific genes (λ5 and VpreB) and the immunoglobulin μ chain form a complex that is transported onto the cell surface
23. preB and λ5 pre-B cell-specific genes can associate with each other and with μ heavy chain
24. A critical role of λ5 protein in B cell development
25. Resolution and characterization of pre-pro-B cell stages in normal mouse bone marrow
26. Enumeration and characterization of DJH structures in mouse fetal liver
27. RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement
28. B-lineage cells in μ-transgenic scid mouse proliferate in response to IL-7 but fail to show evidence of immunoglobulin light chain gene rearrangement
29. Two murine natural polyreactive autoantibodies are encoded by non-mutated germ-line genes
30. A B cell-deficient mouse by targeted disruption of the membrane exon of the immunoglobulin μ chain gene
31. Regulated progression of a cultured pre-B-cell line to the B-cell stage
32. Preferential utilization of the most JH-proximal VH gene segments in pre-B cell lines
33. H replacement during pre-B differentiation: non-random usage of gene segments
34. H genes occurs in the newly generated repertoire of neonatal and adult mice
35. Studies on the generation of B lymphocytes in fetal liver and bone marrow
36. Ordered rearrangement of immunoglobulin heavy chain variable region segments
37. Most peripheral B cells in mice are ligand selected
38. V region dependent selection of persistent resting peripheral B cells in normal mice
39. Allelic inclusion in a pre-B cell line that generates immunoglobulin heavy chain genes in vitro
40. H joining mechanism in heavy-chain-negative (null) pre-B cells results in heavy-chain production
41. + B-cell lymphoma
42. Pre-B cells in mouse bone marrow: Immunofluorescence stathmokinetic studies of the proliferation of cytoplasmic μ-chain-bearing cells in normal mice
43. Proliferation kinetics and the lifespan of B cells in central and peripheral lymphoid organs
44. The regulated expression of B lineage associated genes during B cell differentiation in bone marrow and fetal liver
45. Developmental regulation of the Bcl-2 protein and susceptibility to cell death in B lymphocytes
46. Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
47. Reading of D genes in variable frames as a source of antibody diversity
48. H-joining: implications for the joining mechanism and the ontogenic timing of Ly1 B cell and B-CLL progenitor generation
49. Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences
50. H-reading frame 2 suppression in mouse precursor B cells
51. Cell surface expression of the short immunoglobulin μ chain (Dμ protein) in murine pre-B cells is differently regulated from that of the intact μ chain
52. Conjugation of fluorochromes, haptens, and phycobiliproteins to antibodies
53. High gradient magnetic cell separation with MACS
54. HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells
55. H fusions
56. Insertion of N regions into heavy-chain genes is correlated with expression of terminal deoxytransferase in B cells
57. Junctional sequences of T cell receptor γδ genes: Implications for γδ T cell lineages and for a novel intermediate of V-(D)-J joining