Luminescence Resonance Energy Transfer

Journal of the American Chemical Society

Volumn 116, Issue 13, 1994, Pages 6029-6030

  Selvin, Paul R ^a,b   Rana, Tariq M ^b   Hearst, John E ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHELATION; COMPLEXATION; DYES; EUROPIUM COMPOUNDS; LUMINESCENCE; RESONANCE; SPECTROSCOPIC ANALYSIS;

EUROPIUM CHELATES; FORSTER THEORY; LUMINESCENCE RESONANCE ENERGY TRANSFER; ORGANIC DYE CY-5;

ORGANOMETALLICS;

EID: 0028459554     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja00092a088     Document Type: Article

References (13)

1. Cantor, C. R.; Schimmel, P. R. Biophysical Chemistry; W. H. Freeman and Co.: San Francisco, 1980; Vol. 2.
2. Lanthanides in energy transfer have primarily been used in diffusion-enhanced FRET (Stryer, L.; Thomas, D. D.; Meares, C. F. In Annual Review of Biophysics and Bioengineering; L. J. Mullins, Ed.; Annual Reviews, Inc.: Palo Alto, CA, 1982; Vol. 11; pp 203-222). They have also used been used with multichromophoric allophycocyanin (Mathis, G. Clin. Chem. 1993, 39, 1953) and as isomorphous replacements in calcium-binding proteins (Horrocks, W. D., Jr.; Holmquist, B.; Vallee, B. L. Proc. Natl. Acad. Sci. U.S.A. 1975, 72,4764. Cronce, D. T.; Horrocks, W. D., Jr. Biochemistry 1992, 31, 7963).
3. Stryer, L. Annu. Rev. Biochem. 1978, 47, 819-846.
4. The ability to measure energy transfer well beyong Ro using sensitized emission has been shown by Bruno and Horrocks. They used terbium as the acceptor and tyrosine as the donor. With an. Ro of 3 A they measured out to 12 A. (Bruno, J.; Horrocks, W. D., Jr.; Zauhar, R. J. Biochemistry 1992, 31. 7016.)
5. Selvin, P. R.; Hearst, J. E. Proc. Natl. Acad. Sci. U.S.A., in press.
6. Cardullo, R.; Agrawal, S.; Flores, C.; Zamecnik, P. C.; Wolf, D. E. Proc. Natl. Acad. Sci. U.S.A. 1988, 85, 8780.
7. Clegg, R. M.; Murchie, A. I.; Zechel, A.; Lilley, D. M. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 2994.
8. 2O, the quantum yield is decreased because there are 1.3 water molcules in the primary coordination sphere of the lanthanide in our chelate (data not shown) (Horrocks, W. D. Jr.: Sudnick. D. R. J. Am. Chem. Soc. 1979. 101. 334
9. Bunzli, J.-C.G. In Lanthanide Probes in Life, Chemical and Earth Sciences, Theory and Practice; Bunzli, J.-C.G., Choppin, G. R., Eds.; Elsevier: New York, 1989; pp 219-293.
10. Dexter, D. L. J. Chem. Phys. 1953, 21, 836.
11. Morrison has used gated integration to increase the signal to background of the sensitized emission with organic dyes. Morrison, L. E. Anal. Biochem. 1988, 174, 101.
12. Bailey, M. P.; Rocks, B. F.; Riley, C. Analyst 1984, 109, 1449.
13. Mujumdar, R. B.; Ernst, L. A.; Mujumdar, S. R.; Lewis, C. J.; Waggoner, A. S. Bioconjugate Chem. 1993, 4, 105.