Synthesis of aspartame precursor with an immobilized thermolysin in tert‐amyl alcohol

Biotechnology and Bioengineering

Volumn 43, Issue 11, 1994, Pages 1118-1123

  Nagayasu, Takeshi ^a   Miyanaga, Masamitsu ^a   Tanaka, Takaaki ^a   Sakiyama, Takaharu ^a   Nakanishi, Kazuhiro ^a  

^a OKAYAMA UNIVERSITY   (Japan)

Author keywords

aspartame precursor; enzymatic synthesis; immobilized enzyme; peptide synthesis; thermolysin

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; ENZYMES;

ENZYMATIC SYNTHESIS; OLIGOPEPTIDES; THERMOLYSIN;

ENZYME KINETICS;

2 METHYL 2 BUTANOL; ASPARTAME; THERMOLYSIN; UNCLASSIFIED DRUG;

ARTICLE; ENZYME CHEMISTRY; ENZYME IMMOBILIZATION; PEPTIDE SYNTHESIS;

EID: 0028434202     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.260431116     Document Type: Article

References (13)

1. Role of calcium in the thermal stability of thermolysin
2. Enzymatic Synthesis of the precursor of Leu‐enkephalin in water‐immiscible organic solvent systems
3. Continuous synthesis of a tripeptide by successive condensation and transesterification catalyzed by two immobilized proteinases in organic solvent
4. A new approach to preparative enzymatic synthesis
5. Synthesis of dipeptide precursors with an immobilized enzyme in ethyl acetate
6. Continuous synthesis of N‐(benzyloxycarbonyl)‐L‐aspartyl‐L‐phenylalanine methyl ester with immobilized thermolysin in an organic solvent
7. Design of proteinase catalyzed synthesis of oligopeptides in an aqueous–organic biphasic system
8. Recent developments in enzymatic synthesis of peptide
9. Repeated batch and continuous syntheses of N‐(benzyloxycarbonyl)‐L‐phenylalanyl‐L‐phenylalanine methyl ester with immobilized thermolysin
10. Long‐term continuous synthesis of aspartame precursor in a column reactor with an immobilized thermolysin
11. Thermostable protease from thermophilic bacteria. II. Studies on the stability of the protease
12. Enzymatic synthesis of L‐glutamic acid dimer precursor with immmobilized papain in an orgainc solvent