A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay

Journal of Immunological Methods

Volumn 170, Issue 2, 1994, Pages 211-224

  Ansar Ahmed, S ^a   Gogal Jr , Robert M ^a   Walsh, Jane E ^a  

^a CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

Author keywords

Alamar Blue; Lymphocyte proliferation; Non radioactive; Thymidine incorporation

Indexed keywords

CD4 ANTIGEN; CD45 ANTIGEN; DYE; HERMES ANTIGEN; THYMIDINE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LINE; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HYBRIDOMA; LYMPHOCYTE PROLIFERATION; MACROPHAGE; MOUSE; MYELOMA CELL; NONHUMAN; PRIORITY JOURNAL;

ANIMAL; ANTIBODY FORMATION; ANTIGENS, CD3; CELL LINE; CELL SURVIVAL; CELLS, CULTURED; CONCANAVALIN A; DYES; FLOW CYTOMETRY; HYBRIDOMAS; KINETICS; LYMPHOCYTE ACTIVATION; MACROPHAGES; MICE; MICE, INBRED C57BL; MONOCYTES; MULTIPLE MYELOMA; SPLEEN; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.; T-LYMPHOCYTES; THYMIDINE; THYMUS GLAND; TUMOR CELLS, CULTURED;

ANIMALIA; MURINAE;

EID: 0028344958     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(94)90396-4     Document Type: Article

References (20)

1. Effects of short term administration of sex hormones on normal and autoimmune mice
2. Genetic regulation of testosterone-induced immune suppression
3. Estrogen induces normal murine CD5-positive B cells to produce autoantibodies
4. Immunologic studies of a horse with lymphosarcoma
5. Measurement of human and murine IL-2 and IL-4
6. Changes in macrophage Mac antigen expression during tumor growth
7. Measurement of antigen specific lymphocyte proliferation using 5-bromo-deoxyuridine incorporation
8. Oxidoreductase
9. Identification of macrophage-like characteristics in a cultured murine tumor line
10. Measurement of cell numbers by means of endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cellular surface antigen
11. Detection of human white cell proliferative responses by immunoenzymatic measurement of bromodeoxyuridine uptake
12. 3H) thymidine techniques to measure cell proliferation
13. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays
14. A new flurometric assay for cytotoxicity measurements in vitro
15. Quantitation of 5-bromo-2-deoxyuridine incorporation into DNA: an enzyme immunoassay for the assessment of lymphoid proliferative response
16. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines
17. Phagocytosis and cytolysis by a macrophage tumor and its cloned cell line
18. An improved colorimetric assay for cell proliferation and viability utilizing tetrazolium salt XTT
19. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines
20. Measurement of lymphocyte proliferation: Critical analysis of radioactive and photometric methods