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2) by a carbonate buffer adsorption method as described (7). Primary rat hepatocytes were isolated and cultured in defined medium on the laminin-coated substrata as described (7).
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84901965477
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note
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We thank M. Fontaine for performing the rat liver perfusions and hepatocyte isolation and O. Hurtado for technical assistance in preparing the stamp template. Supported by NSF cooperative agreement (EEC-8803014) to the Biotechnology Process Engineering Center at MIT; the Office of Naval Research and the Advanced Projects Research Agency (Harvard University); and an American Cancer Society research grant (CD-493) (D.E.I.). R.S. is a recipient of a fellowship from Merck & Co., and D.E.I, is a recipient of a Faculty Research Award from the American Cancer Society.
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