Kinetic Studies on a Genetically Engineered Fused Enzyme between Rat Cytochrome P4501A1 and Yeast NADPH-P450 Reductase

Biochemistry

Volumn 33, Issue 16, 1994, Pages 4933-4939

  Sakaki, Toshiyuki ^a   Akiyoshi Shibata, Megumi ^a   Yabusaki, Yoshiyasu ^a   Kominami, Shiro ^b   Takemori, Shigeki ^b   Ohkawa, Hideo ^c  

^a SUMITOMO CHEMICAL CO LTD   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

^c KOBE UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE FERRIHEMOPROTEIN REDUCTASE;

ANIMAL CELL; ARTICLE; BINDING SITE; ELECTRON TRANSPORT; ENDOPLASMIC RETICULUM; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; HYDROPHOBICITY; HYDROXYLATION; MICROSOME; NONHUMAN; PRIORITY JOURNAL; RAT; SPECTROMETER;

ANIMAL; BASE SEQUENCE; CATALYSIS; CYTOCHROME B5; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; HEME; HYDROXYLATION; KINETICS; MICROSOMES; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; NADPH-FERRIHEMOPROTEIN REDUCTASE; OXIDATION-REDUCTION; RATS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; ZOXAZOLAMINE;

ANIMALIA; SACCHAROMYCES CEREVISIAE;

EID: 0028308783     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi00182a023     Document Type: Article

References (42)

1. Aoyama, Y., Yoshida, Y., Kubota, S., Kumaoka, H., & Furumichi, A. (1978) Arch. Biochem. Biophys. 185, 362–369.
2. Boddupalli, S. S., Oster, T., Estabrook, R. W., & Peterson, J. A. (1992) J. Biol. Chem. 267, 10375–10380.
3. Causey, K. C., Eyer, C. S., & Blackes, W. L. (1990) Mol. Pharmacol. 38, 134–142.
4. Eyer, C. S., & Backes, W. (1992) Arch. Biochem. Biophys. 293, 231–240.
5. Fisher, C. W., Shet, M. S., Caudle, D. L., Martin-Wixtrom, C. A., & Estabrook, R. W. (1992) Proc. Natl. Acad. Sei. U.S.A. 89, 10817–10821.
6. Fulco, A. J., & Ruttinger, R. T. (1987) Life Sei. 40, 1769–1775.
7. Guengerich, F. P., Ballou, D. P., & Coon, M. J. (1976) Biochem. Biophys. Res. Commun. 70, 951–956.
8. Halpert, J. R., & He, Y. (1993) J. Biol. Chem. 268, 4453–4457.
9. Imai, Y., & Nakamura, M. (1989) Biochem. Biophys. Res. Commun. 158, 717–722.
10. Imai, Y., Sato, R., & Iyanagi, T. (1977) J. Biochem. 82, 1237–1246.
11. Iwase, T., Sakaki, T., Yabusaki, Y., Ohkawa, H., Ohta, Y., & Kawato, S. (1991) Biochemistry 30, 8347–8351.
12. Iwasaki, M., Darden, T. A., Pederson, L. G., Davis, D. G., Juvonen, R. O., Sueyoshi, T., & Negishi, M. (1993) J. Biol. Chem. 268, 759–762.
13. Iyanagi, T., Makino, R., & Anan, K. (1981) Biochemistry 20, 1722–1730.
14. Kominami, S., Hara, H., Ogishima, T., & Takemori, S. (1984) J. Biol. Chem. 259, 2991–2999.
15. Kominami, S., Inoue, S., Higuchi, A., & Takemori, S. (1989) Biochim. Biophys. Acta 985, 293–299.
16. Kominami, S., Ogawa, N., Morimune, R., De-Ying, H., & Takemori, S. (1992) J. Steroid Biochem. Mol. Biol. 42, 57–64.
17. Larson, J. R., Coon, M. J., & Porter, T. D. (1991) J. Biol. Chem. 266, 7321–7324.
18. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951) J. Biol. Chem. 193, 265–275.
19. Murakami, H., Yabusaki, Y., & Ohkawa, H. (1986) DNA 5, 1–10.
20. Murakami, H., Yabusaki, Y., Sakaki, T.,Shibata, M., & Ohkawa, H. (1987) DNA 6, 189–197.
21. Murakami, H., Yabusaki, Y., Sakaki, T., Shibata, M., & Ohkawa, H. (1990) J. Biochem. 108, 859–865.
22. Narhi, L. O., & Fulco, A. J. (1986) J. Biol. Chem. 261, 7160–7169.
23. Nelson, D. R., Kamataki, T., Waxman, D. J., Guengerich, F. P., Estabrook, R. W., Feyereisen, R., Gonzalez, F. J., Coon, M. J., Gunsalus, I. C., Gotoh, O., Okuda, K., & Nebert, D. W. (1993) DNA Cell Biol. 12, 1–51.
24. Oeda, K., Sakaki, T., & Ohkawa, H. (1985) DNA 4, 203–210.
25. Ohta, Y., Sakaki, T., Yabusaki, Y., Ohkawa, H., & Kawato, S. (1994) J. Biol. Chem. (in press).
26. Omura, T., & Sato, R. (1964) J. Biol. Chem. 239, 2370–2378.
27. Peterson, J. A., & Bodddupalli (1992) Arch. Biochem. Biophys. 294, 654–661.
28. Peterson, J. A., Ishimura, Y., & Griffin, B. W. (1972) Arch. Biochem. Biophys. 149, 197–208.
29. Peterson, J. A., Ebel, R. E., O'Keeffe, D. H., Matsubara, T., & Estabrook, R. W. (1976) J. Biol. Chem. 251, 4010–4016.
30. Raag, R., Martinis, S. A., Sliger, S. G., & Poulos, T. L. (1991) Biochemistry 30, 11420–11429.
31. Ruettinger, R. T., Wen, L. P., & Fulco, A. J. (1989) J. Biol. Chem. 264, 10987–10995.
32. Sakaki, T., Shibata, M., Yabusaki, Y., Murakami, H., & Ohkawa, H. (1987) DNA 6, 31–39.
33. Sakaki, T., Shihata, M., Yabusaki, Y., Murakami, H., & Ohkawa, H. (1990) DNA Cell Biol. 9, 603–614.
34. Sakaki, T., Akiyoshi-Shibata, M., Yabusaki, Y., Manabe, K., Murakami, H., & Ohkawa, H. (1991) Pharmacogenetics 1, 86–93.
35. Shibata, M., Sakaki, T., Yabusaki, Y., Murakami, H., & Ohkawa, H. (1990) DNA Cell Biol. 9, 27–36.
36. Strittmater, P., & Velick, S. F. (1956) J. Biol. Chem. 221, 253–264.
37. Taniguchi, H., Imai, Y., & Sato, P. (1984) Arch. Biochem. Biophys. 232, 585–596.
38. Tomaszewski, J. E., Jerina, D. M., Levin, W., & Conney, A. H. (1976) Arch. Biochem. Biophys. 176, 788–798.
39. Yabusaki, Y., & Ohkawa, H. (1991) in Frontiers in Biotransformation (Ruckpaul, K., & Rein, H., Eds.) Vol. 4, pp 16–190, Akademie Verlag, Berlin.
40. Yabusaki, Y., Murakami, H., Sakaki, T., Shibata, M., & Ohkawa, H. (1988a) DNA 7, 701–711.
41. Yabusaki, Y., Murakami, H., & Ohkawa, H. (1988b) J. Biochem. 103, 1004–1010.
42. Zuber, M. X., John, M. E., Okamura, T., Simpson, E. R., & Waterman, M. R. (1986) J. Biol. Chem. 261, 2475–2482.