-
10
-
-
0027177465
-
Line Scanning System for Direct Digital Chemiluminescence Imaging of DNA Sequencing Blots
-
of special interest, A system for high throughput scanning of chemlluminescent labeled DNA separations with using a time-integrated cooled CCD is demonstrated.
-
(1993)
Anal Chem
, vol.65
, pp. 1785-1793
-
-
Karger1
Weiss2
Gesteland3
-
11
-
-
0026823566
-
Development of Resonance Ion Spectroscopy for DNA Sequencing and Genome Mapping
-
of special interest, Isotopes of tin are used to label DNA fragments generated using Sanger sequencing reactions. Resonance ion spectroscopy is used to detect and discriminate DNA bands after electrophoretic separate in polyacrylamide gels.
-
(1992)
Analytical Chemistry
, vol.64
, pp. 315-328
-
-
Jacobson1
Arlinghaus2
-
13
-
-
14744282674
-
High Speed Automated DNA Sequencing in Ultrathin Slab Gels
-
of outstanding interest, A horizontal electrophoretic ultra-thin gels system is used to separate the DNA fragments generated by Sanger sequencing reactions. A four-color fluorescent detection system is used to read the gels.
-
(1992)
Biotechnology
, vol.10
, pp. 78-81
-
-
Kostichka1
Marchbanks2
Brumley3
Drossman4
Smith5
-
14
-
-
0027918108
-
Optimization of Electric Field Strength for DNA Sequencing in Capillary Gel Elcctrophorcsis
-
•]. These models are used to determine the optimal field strength for capillary separations.
-
(1993)
Anal Chem
, vol.65
, pp. 2841-2850
-
-
Luckey1
Smith2
-
15
-
-
0027120996
-
DNA Sequencing Using Capillary Array Electrophoresis
-
of outstanding interest, Capillary arrays are used to separate the DNA fragments generated by Sanger sequencing reactions. Separations can be performed 25 times faster than with traditional slab gels. A binary fluorescent dye system is used to detect the fragments.
-
(1992)
Anal Cbem
, vol.64
, pp. 2149-2154
-
-
Huang1
Quesada2
Mathies3
-
16
-
-
0000288222
-
Capillary Array Electrophoresis Using Laser-Excited Confocal Fluorescence Detection
-
−12 mol) of capillary array electrophoretic separations is demonstrated.
-
(1992)
Anal Chem
, vol.64
, pp. 967-972
-
-
Huang1
Quesada2
Mathies3
-
17
-
-
0027294978
-
A Model for the Modility of Single Stranded DNA in Capillary Gel Electrophoresis
-
•].
-
(1993)
Electrophoresis
, vol.14
, pp. 492-501
-
-
Luckey1
Smith2
-
22
-
-
84914808073
-
Matrix-Assisted Laser Desorption Mass Spectrometry of Homopolymcr Oligodeoxyribonucleotides. Influence of Base Composition on the Mass Spectrometric Response
-
in press
-
(1994)
Org Mass Spec
, pp. 28
-
-
Schneider1
Chair2
-
23
-
-
0027690253
-
Basic Matrices for the Matrix-Assisted Laser Desorption/lonization Mass Spectrometry of Protein and Oligonucleotides
-
(1993)
Anal Chem
, vol.65
, pp. 3204-3210
-
-
Fitzgerald1
Zhu2
Smith3
-
24
-
-
0027551428
-
Matrix-Assisted Laser Desorption Time-of-flight Mass Spectrometry of Oligonucleotides Use 3-Hydropicolinic Acid as an Ultraviolet Sensitive Matrix
-
of outstanding interest, Effective matrix-assisted laser desorption time-of-flight mass spectroscopy separation of homopolymers and heteropolymers of all four bases is demonstrated using 3-hydropicolinic acid as the matrix.
-
(1993)
Rapid Commun Mass Spectrom
, vol.7
, pp. 142
-
-
Wu1
Steding2
Becker3
-
25
-
-
84990647399
-
The Analysis of Mock DNA Sequencing Reactions Using Matrix-Assisted Laser Dcsorption/lonization Mass Spectrometry
-
of outstanding interest, Four mixtures simulating the nested fragments resulting from Sanger sequencing reactions are succesfully separated and detected using matrix-assisted laser desorption time-of-flight mass spectrometry and 3-hydropicolinic acid as the matrix.
-
(1993)
Rapid Communications in Mass Spectrometry
, vol.7
, pp. 895-897
-
-
Fitzgerald1
Zhu2
Smith3
-
26
-
-
0024263226
-
A Novel Method for Nucleic Acid Se-quence Determination
-
(1988)
J Theor Biol
, vol.135
, pp. 303-307
-
-
Bains1
Smith2
-
30
-
-
0026742791
-
Hydridization with Olignucleotide Arrays
-
of outstanding interest, DNA probe matrices of 256 G-T eight-mers are synthesized using a physical masking strategy. Reconstruction of 20-mer targets and comparitive sequencing based upon data from hybridization of targets matrices is described.
-
(1992)
Genomics
, vol.13
, pp. 1008-1017
-
-
Southern1
Maskos2
Elder3
-
32
-
-
0027909781
-
Multiplexed Biochemical Assays with Biological Chips
-
of outstanding interest, Methods combining high-resolution photolithography and solid-state chemical synthesis to construct high density arrays of biopolymers are described. Confocal laser microscopy is used to detect the binding of target molecules to the surface.
-
(1993)
Nature
, vol.364
, pp. 555-556
-
-
Fodor1
Rava2
Huang3
Pease4
Holmes5
Adams6
-
33
-
-
0028246289
-
Light-Generated Oligonucleotidc Arrays for Rapid DNA Sequence Analysis
-
of outstanding interest, Photolithography and solid state synthesis are used to contruct arrays of DNA probes. The availability of the probes for hybridization is demonstrated. Arrays of 256 eight-mer probes are used to demonstrate hybridization specificity.
-
(1994)
Proceedings of the National Academy of Sciences
, vol.91
, pp. 5022-5026
-
-
Pease1
Solas2
Sullivan3
Cronin4
Holmes5
Fodor6
-
34
-
-
21144469589
-
DNA Sequencing by Hybridization with Oligonucleotide Matrix (SHOM). Theory of Washing out the DNA After Hybridization
-
of special interest, DNA probes are immobilized in an acrylamide matrix, and target DNA fragments are inncubated with the matrix. From this a model that describes the dependence of hybridization rate and melting temperature on the immobilized probe concentration is obtained.
-
(1992)
Mol Biol
, vol.6
, pp. 856-865
-
-
Livshitz1
Ivanov2
Mirzabekov3
Florent4
-
35
-
-
36448999220
-
Detection and Lifetime Measurement of Single Molecules in Flowing Sample Streams by Laser-induced Fluorecence
-
of special interest, Detection and measurement of fiuoresence lifetimes of single rhodamine-110 molecules in flowing in an aqueous sample stream are demonstrated.
-
(1993)
Appl Phys Lett
, vol.62
, pp. 2030-2032
-
-
Wilkerson1
Goodwin2
Ambrose3
Martin4
Keller5
-
36
-
-
84989749019
-
The Photophysical Constants of Several Fluorescent Dyes Pertaining to Ultrasensitive Fluorescent Spectroscopy
-
of special interest, The fluorescence quantum efficiency, photodestruction quantum efficiency, absorption cross section and fluoresence lifetime of rhodamine 6G, tetramethylrhodamine-5-isothiocynate and Texas Red in aqueous solvents, ethanol, and cationic surfactants are reported.
-
(1993)
Photochemistry and Photobiology
, vol.57
, pp. 972-977
-
-
Soper1
Nutter2
Keller3
Davis4
Shera5
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