Relationship between water activity and catalytic activity of lipases in organic media: Effects of supports, loading and enzyme preparation

European Journal of Biochemistry

Volumn 222, Issue 2, 1994, Pages 461-466

  VALIVETY, Rao H ^a   HALLING, Peter J ^a   PEILOW, Alan D ^b   MACRAE, Alasdair R ^b  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^b UNILEVER RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ORGANIC COMPOUND; TRIACYLGLYCEROL LIPASE;

ARTICLE; CANDIDA RUGOSA; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; FUNGUS; PRIORITY JOURNAL;

CANDIDA; CATALYSIS; COMPARATIVE STUDY; ENZYMES, IMMOBILIZED; HEXANES; KINETICS; LAURIC ACIDS; LIPASE; RHIZOBIACEAE; SUPPORT, NON-U.S. GOV'T; WATER;

EID: 0028282678     PISSN: 00142956     EISSN: 14321033     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1994.tb18886.x     Document Type: Article

References (16)

1. On the importance of the support material for enzymatic synthesis in organic media. Support effects at controlled water activity
2. Activation of enzymes in organic media at low water activity by polyols and saccharides
3. Blueprint for a lipase support: use of hydrophobic controlled‐pore glasses as model systems
4. Effect of water on equilibria catalysed by hydrolytic enzymes in biphasic reaction systems
5. Organic liquids and biocatalysts: theory and practice
6. Physical‐chemical nature of low water systems for biocatalysis: especially phase behaviour, water activity and pH
7. Enzymatic peptide synthesis via segment condensation in the presence of water mimics
8. Cloning and nucleotide sequences of two lipase genes from Candida cylindracea
9. Zur Wasser‐bindung adsorptiv immobilisierter Lipasen
10. Reaction rates in organic media show similar dependence on water activity with lipase catalyst immobilized on different supports
11. On the importance of the support material for bioorganic synthesis. Influence of water partition between solvent, enzyme and solid support in water‐poor reaction media
12. Purification and characterization of 2 distinct lipases from Candida cylindracea
13. Enzymatic acyl exchange of triglycerides in hexane
14. Reaction rate with lipase catalyst shows similar dependence on water activity in different organic solvents
15. Lipases from different sources vary widely in dependence of catalytic activity on water activity
16. Rhizomucor miehei lipase remains highly active at water activity below 0. 0001