Colorimetric evaluation of cultured osteoblast-like cells (ROS 17/2.8)

Biotechnic and Histochemistry

Volumn 69, Issue 3, 1994, Pages 160-164

  Genty, Christian ^a   Palle, Sabine ^a   Vanelle, Laurence ^a   Bourrin, Sandrine ^a   Alexandre, Christian ^a  

^a University of Medicine   (France)

Author keywords

Cell quantification; Osteoblast like cells. methylene blue; Scanning microscopy; Spectrophotometry

Indexed keywords

METHYLENE BLUE;

ARTICLE; CELL COUNT; CELL CULTURE; CELL STRUCTURE; COLORIMETRY; OSTEOBLAST; PROTEIN CONTENT; SPECTROPHOTOMETRY; STAINING;

ANIMAL; CELL COUNT; COLORIMETRY; COMPARATIVE STUDY; DNA, NEOPLASM; EVALUATION STUDIES; METHYLENE BLUE; MICROSCOPY, ELECTRON, SCANNING; MODELS, BIOLOGICAL; NEOPLASM PROTEINS; OPTICS; OSTEOBLASTS; OSTEOSARCOMA; SPECTROPHOTOMETRY; THYMIDINE; TRITIUM; TUMOR CELLS, CULTURED;

EID: 0028272438     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.3109/10520299409106279     Document Type: Article

References (14)

1. Establishment of an osseous line from fetal rat calvaria using an immunocytolytic method of cell selection: characterization of the cell line and of derived clones
2. Comparison of fixation and drying procedures for preparation of some cultured cell lines for examination in the SEM
3. 3 on cultured osteoblasts from mature rats
4. The effects of recombinant human interleukin-18 on cellular proliferation and the production of prostaglandin E2, plasminogen activator, osteocalcin and alkaline phosphatase by osteoblast-like cells derived from human bone
5. Spectrophotometric quantification of anchorage-dependent cell numbers using extraction of naphthol blue-black-stained cellular protein
6. A semiautomated microculture method for investigating growth inhibitory effects of cytotoxic compounds and exponentially growing carcinoma cells
7. Relationship between collagen synthesis and expression of the osteoblast phenotype in MC3T3–E1 cells
8. Subclone heterogeneity in a clonally-derived osteoblast-like cell line
9. Parathyroid hormone responsive clonal cell lines from rat osteosarcoma
10. Evaluation of growth rate in adhering cell cultures using a simple colorimetric method
11. Expression of the osteoblastic phenotype
12. Evaluation of different staining procedures for the quantification of fibroblasts cultured in 96-well plates
13. Refinement of the Coomassie blue method of protein quantification