Copurification of E. coli RNAase E and PNPase: Evidence for a specific association between two enzymes important in RNA processing and degradation

Cell

Volumn 76, Issue 5, 1994, Pages 889-900

  Carpousis, Agamemnon J ^a,b   Van Houwe, Griet ^a   Ehretsmann, Claude ^a   Krisch, Henry M ^b  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE; RIBONUCLEASE; RNA;

ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; RNA DEGRADATION; RNA PROCESSING;

EID: 0028269435     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/0092-8674(94)90363-8     Document Type: Article

References (32)

1. ATG vectors for regulated high-level expression of cloned genes in E coli
2. The ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene
3. Transcription and mRNA processing upstream of bacteriophage T4 gene 32
4. Cloning and analysis of the entire E. coli ams gene
5. Sequencing and expression of the rne gene of E coli
6. Analysis of the altered mRNA stability (ams) gene from E coli
7. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis
8. Structural requirements for the processing of E coli 5S ribosomal RNA by RNAase E in vitro
9. Enzymatic basis for hydrolytic versus phosphorolytic mRNA degradation in E coli and B subtilis
10. Polynucleotide phosphorylase and RNAse II are required for cell viability and mRNA turnover in E coli K-12
11. Specificity of E coli endoribonuclease RNAase E in vivo and in vitro analysis of mutants in a bacteriophage T4 mRNA processing site
12. mRNA degradation in prokaryotes
13. The nature of information required for export and sorting present within the outer membrane protein ompA of E coli K-12
14. Decay of ompA mRNA and processing of 9S RNA are immediately affected by shifts in growth rate but in opposite manners
15. Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E coli
16. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels removal of sodium dodecyl sulfate and renaturation of enzyme activity results with sigma subunit of E coli RNA polymerase wheat germ DNA topoisomerase and other enzymes
17. Gene 32 transcription and mRNA processing in T4-related bacteriophages
18. Cleavages in the 5′ region of the ompA and bla mRNA control stability studies with an E coli mutant altering mRNA stability and a novel endonuclease
19. mRNA degradation by processive 3′–5′ exoribonucleases in vitro and the implications for prokaryotic mRNA decay in vivo
20. Genetic studies of cleavage-initiated mRNA decay and processing of 9S RNA show that the E coli ams and me loci are the same
21. Location of the RNA-processing enzymes RNAase III RNAase E and RNAase P in the E coli cell
22. RNAase E an RNA processing enzyme from E coli
23. Gene rne affects the structure of the ribonucleic acid processing enzyme ribonuclease E of E coli
24. E coli endoribonuclease RNAase E autoregulation of expression and site-specific cleavage of mRNA
25. E coli RNAase E has a role in the decay of bacteriophage T4 mRNA
26. RNAase E an endoribonuclease has a general role in the chemical decay of E coli mRNA evidence that rne and ams are the same genetic locus
27. A conditional lethal mutation in an E coli strain with a longer chemical lifetime of mRNA
28. Control of functional mRNA stability in bacteria multiple mechanisms of nucleolytic and non-nucleolytic inactivation
29. Quaternary structure of polynucleotide phosphorylase from E coli evidence for a complex between two types of polypeptide chains
30. Nucleotide sequence of the pnp gene of E coli encoding the catalytic subunit of polynucleotide phosphorylase
31. Maturation of 5S ribosomal RNA ribonuclease E cleavages and their dependence on precursor sequences
32. Functional interaction of heat shock protein GroEL with an RNAase E-like activity in E coli