A novel radioligand binding assay to determine diagnostic accuracy of isoform-specific glutamic acid decarboxylase antibodies in childhood IDDM

Diabetologia

Volumn 37, Issue 4, 1994, Pages 344-350

  Grubin, C E ^a   Daniels, T ^a   Toivola, B ^a   Landin Olsson, M ^b   Hagopian, W A ^a   Li, L ^a   Karlsen, A E ^c   Boel, E ^d   Michelsen, B ^c   Lernmark, A ^a,e  

^a UNIVERSITY OF WASHINGTON   (United States)

^b LUND UNIVERSITY   (Sweden)

^c HAGEDORN RESEARCH INSTITUTE   (Denmark)

^d NOVO NORDISK A S   (Denmark)

^e KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

autoimmunity; diabetes mellitus; diagnostic accuracy; Glutamic acid decarboxylase; islet cell antibodies; receiver operating characteristic plot

Indexed keywords

GLUTAMATE DECARBOXYLASE; PANCREAS ISLET CELL ANTIBODY;

ADOLESCENT; ARTICLE; AUTOIMMUNITY; CHILD; CONTROLLED STUDY; DIAGNOSIS; FEMALE; HUMAN; INSULIN DEPENDENT DIABETES MELLITUS; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL;

ADOLESCENT; ADULT; AUTOANTIBODIES; AUTOIMMUNITY; CHILD; DIABETES MELLITUS, TYPE 1; FALSE POSITIVE REACTIONS; FEMALE; GLUTAMATE DECARBOXYLASE; HUMANS; ISLETS OF LANGERHANS; ISOENZYMES; MALE; MIDDLE AGED; PREDICTIVE VALUE OF TESTS; RADIOLIGAND ASSAY; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY;

EID: 0028258006     PISSN: 0012186X     EISSN: 14320428     Source Type: Journal    
DOI: 10.1007/BF00408469     Document Type: Article

References (40)

1. Islet cell antibodies-theoretical and practical implications
2. Autoantibodies in newly diagnosed diabetic children immunoprecipitate human pancreatic islet cell proteins
3. Antibodies to a Mr 64,000 islet cell protein in Swedish children with newly diagnosed type 1 (insulin-dependent) diabetes
4. Antibodies to a Mr 64,000 human islet cell antigen precede the clinical onset of insulin-dependent diabetes
5. 64,000 Mr autoantibodies as predictors of insulin-dependent diabetes
6. Cloning and primary structure of a human islet isoform of glutamic acid decarboxylase from chromosome 10
7. Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are each encoded by a single gene
8. Brain glutamate decarboxylase (GAD) gene assigned to human chromosome 2 by somatic cell hybrid analysis
9. Cloning, characterization, and autoimmune recognition of rat islet glutamic acid decarboxylase in insulin-dependent mellitus
10. Glutamic acid decarboxylase: an autoantigen in insulin-dependent diabetes mellitus
11. Auto-antibodies in IDDM primarily recognize the 65,000-Mr rather than the 67,000-Mr isoform of glutamic acid decarboxylase
12. Autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus
13. Differential expression of glutamic acid decarboxylase in rat and human islets
14. Antibodies to glutamic acid decarboxylase discriminate major types of diabetes mellitus
15. An ELISA for antibodies to recombinant glutamic acid decarboxylase in IDDM
16. Islet cell cytoplasmic autoantibody reactivity to glutamate decarboxylase in insulin-dependent diabetes
17. Demonstration of GAD-65 as the main immunogenic isoform of glutamate decarboxylase in type 1 diabetes and determination of autoantibodies using a radioligand produced by eukaryotic expression
18. Autoantibodies to glutamic acid decarboxylase (GAD) detected by an immunotrapping enzyme activity assay: relation to insulin dependent diabetes mellitus and islet cell antibodies
19. Prevalence of autoantibodies to the 65- and 67-kD isoforms of glutamate decarboxylase in insulin-dependent diabetes mellitus
20. Recombinant glutamic acid decarboxylase representing a single isoform expressed in human islets detects IDDM association 65K autoantibodies
21. A prospective study of indexes redicting the outcome of trials of weaning from mechanical ventilation
22. Basic principle of ROC analysis
23. Estimating mortality risk in preoperative patients using immunologic, nutritional, and acute-phase response variables
24. Receiver-operating characteristics (ROC) plots: a fundamental tool in clinical medicine
25. Plasmapheresis in the initial treatment of insulin-dependent diabetes mellitus in children
26. Islet cell antibodies in insulin-dependent (type 1) diabetic children treated with plasmapheresis
27. Immunology and diabetes workshops: report of the second international workshop on the standardization of cytoplasmic islet cell antibodies
28. Immunology and diabetes workshops: report of the first international workshop on the standardisation of cytoplasmic islet cell antibodies
29. Prolonged incubation in the two-colour immunofluorescence test increases the prevalence and titres of islet cell antibodies in type 1 (insulin-dependent) diabetes mellitus
30. Islet cell and other organ-specific autoantibodies in all children developing type 1 (insulindependent) diabetes mellitus in Sweden during one year and in matched controls
31. The Swedish childhood diabetes study — results from a nine year case register and one year case-referent study indicating that type 1 (insulin-dependent) diabetes mellitus is associated with both type 2 (non-insulin-dependent) diabetes mellitus and autoimmune disorders
32. Monoclonal antibodies to glutamic acid decarboxylase
33. Serum exchange and use of dilutions have improved precision of measurement of islet cell antibodies
34. The Fourth International Serum Exchange Workshop to standardize cytoplasmic islet cell antibodies
35. Two genes encode distinct glutamate decarboxylase
36. Quantitative assay using recombinant human islet glutamic acid decarboxylase (GAD65) shows 64K autoantibody positivity at onset predicts diabetes type
37. Pancreatic β cells express two autoantigenic forms of glutamic acid decarboxylase, a 65-kDa hydrophilic form and a 64-kDa amphiphilic form which can be both membrane-bound and soluble
38. Predictive value of islet cell and insulin autoantibodies for type 1 (insulin-dependent) diabetes mellitus in a population-based study of newly diagnosed diabetic and matched control children
39. Risk for developing type 1 (insulin-dependent) diabetes mellitus and the presence of islet 64K antibodies