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1
-
-
0026795613
-
-245
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2-generating NADPH oxidase with purified, relipidated cytochrome and the recombinant protein p47-phox, p67-phox and Rac1. These findings indicated that the NADPH oxidase minimally consists of these four proteins. The Rac1 protein expressed by Escherichia coli required pre-loading with GTPγS to exert activity.
-
(1992)
J Biol Chem
, vol.267
, pp. 16767-16770
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-
Abo1
Boyhan2
West3
Thrasher4
Segal5
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2
-
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0026642394
-
Cytochrome b is a Flavocytochrome Containing FAD and the NADPH-Binding Site of the Microbicidal Oxidase of Phagocytes
-
of outstanding interest, This study presents evidence that the cytochrome b is a flavocytochrome, containing an NADPH binding site, and indicates by sequence comparisons that the flavin- and NADPH-binding regions are located on the gp91-phox subunit of the cytochrome b.
-
(1992)
Biochem J
, vol.284
, pp. 781-788
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-
Segal1
West2
Wientjes3
Nugent4
Chavan5
Haley6
Garcia7
Rosen8
Scrace9
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3
-
-
0026613011
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558: The Flavin-Binding Component of the Phagocyte NADPH Oxidase
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558, recombinant p47-phox and p67-phox from Sf9 insect cells, and a Rac/[Rho]GDI complex purified from neutrophil cytosol. The absolute requirement for FAD for in vitro activity was shown to be dependent on the cytochrome b, thereby identifying it to be a flavocytochrome.
-
(1992)
Science
, vol.256
, pp. 1459-1462
-
-
Rotrosen1
Yeung2
Leto3
Malech4
Kwong5
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5
-
-
0025259712
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Two Cytosolic Components of the Human Neutrophil Respiratory Burst Oxidase Translocate to the Plasma Membrane During Cell Activation
-
(1990)
J Clin Invest
, vol.85
, pp. 714-721
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-
Clark1
Volpp2
Leidal3
Nauseef4
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9
-
-
0025872035
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Nucleoside Triphosphate Requirements for Superoxide Generation and Phosphorylation in a Cell-Free System From Human Neutrophils
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(1991)
J Biol Chem
, vol.266
, pp. 20990-20997
-
-
Uhlinger1
Burnham2
Lambeth3
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11
-
-
0026753247
-
Involvement of GTP in Cell-Free Activation of Neutrophil NADPH Oxidase
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(1992)
Biochem J
, vol.285
, pp. 635-639
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-
Klinger1
Aviram2
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12
-
-
0024848202
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Activation of the Superoxide-Forming NADPH Oxidase of Macrophages Requires Two Cytosolic Components — One of Them is Also Present in Certain Nonphagocytic Cells
-
(1989)
J Immunol
, vol.143
, pp. 4180-4183
-
-
Pick1
Kroizmar2
Abo3
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15
-
-
0026325723
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Purification and Characterization of a Third Cytosolic Component of the Superoxide-Generating NADPH Oxidase of Macrophages
-
(1991)
J Biol Chem
, vol.266
, pp. 23577-23585
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-
Abo1
Pick2
-
16
-
-
0027067903
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Purification and Characterization of Rac2. A Cytosolic GTP-Binding Protein That Regulates Human Neutrophil NADPH Oxidase
-
of special interest, Description of the purification of the cytosolic NADPH oxidase regulatory component Rac2 in human neutrophils and biochemical characterization of its GTP-binding and hydrolysis properties.
-
(1992)
J Biol Chem
, vol.267
, pp. 23575-23582
-
-
Knaus1
Heyworth2
Kinsella3
Curnutte4
Bokoch5
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17
-
-
0026775475
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Regulation of the Superoxide-Generating NADPH Oxidase by a Small GTP-Binding Protein and Its Stimulatory and Inhibitory GDP/GTP Exchange Proteins
-
of outstanding interest, Purification of Rac2 from the cytosol of differentiated HL-60 cells. The superoxide generation by this protein in the cell-free assay was stimulated by smgGDS and inhibited by [Rho]GDI, both regulators of the GTP/GDP state of GTP-binding proteins.
-
(1992)
J Biol Chem
, vol.267
, pp. 10215-10218
-
-
Mizuno1
Kaibuchi2
Ando3
Musha4
Hiraoka5
Takaishi6
Asada7
Nunoi8
Matsuda9
Takai10
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18
-
-
0027288743
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Regulation of the Human Neutrophil NADPH Oxidase by rho-Related G-Proteins
-
of outstanding interest, Purification of a Rac2/[Rho]GDI complex from human neutrophils, generating superoxide in a cell-free assay when added to recombinant p47-phox and p67-phox. Studies with recombinant E. coli proteins showed NADPH oxidase stimulatory activity for Rac1 and Rac2, but not for the related CDC42Hs protein.
-
(1993)
Biochemistry
, vol.32
, pp. 5711-5717
-
-
Kwong1
Malech2
Rotrosen3
Leto4
-
19
-
-
0027467339
-
Requirement for Post-Translational Processing of Rac GTP-Binding Proteins for Activation of Human Neutrophil NADPH Oxidase
-
of outstanding interest, This study compares the NADPH oxidase activity of recombinant Rac1 and Rac2 proteins in post-translationally processed and unprocessed forms, and investigates the effect of the GDP/GTP state of the proteins on their oxidase stimulatory activity. The results demonstrate a requirement for processing for guanine nucleotide exchange, but not for interaction with the other oxidase components.
-
(1993)
Mol Biol Cell
, vol.4
, pp. 261-269
-
-
Heyworth1
Knaus2
Xu3
Uhlinger4
Conroy5
Bokoch6
Curnutte7
-
20
-
-
0027052911
-
Post-Translational Processing of Rac p21s is Important Both for Their Interaction with the GDP/GTP Exchange Proteins and for Their Activation of NADPH Oxidase
-
of special interest, The authors show that post-translational processing is a prerequisite for interaction with the regulatory proteins smgGDS and [Rho]GDI. Furthermore, their results suggest that processing is necessary for the stimulation of the NADPH oxidase in vitro.
-
(1992)
J Biol Chem
, vol.267
, pp. 25709-25713
-
-
Ando1
Kaibuchi2
Sasaki3
Hiraoka4
Nishiyama5
Mizuno6
Asada7
Nunoi8
Matsuda9
Matsuura10
-
21
-
-
0027520431
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Translocation of Rac Correlates with NADPH Oxidase Activation: Evidence for Equimolar Translocation of Oxidase Components
-
of outstanding interest, Determination of the subcellular distribution of the cytosolic oxidase components and [Rho]GDI depending on the activation state of the neutrophil. Rac was shown to translocate upon stimulation with PMA or fMLP in equimolar amounts with p47-phox and p67-phox, while [Rho]GDI stayed completely cytosolic.
-
(1993)
J Biol Chem
, vol.268
, pp. 20983-20987
-
-
Quinn1
Evans2
Priscu3
Jesaitis4
Bokoch5
-
22
-
-
0026662445
-
Inhibition of Superoxide Production in B Lymphocytes by Rac Antisense Oligonucleotides
-
of special interest, Superoxide production in human Epstein-Barr virus-transformed B lymphocytes was found to be dependent on the Rac protein content. Treatment with 16-mer antisense oligonucleotide to the shared amino-terminal sequence of Rac proteins decreased Rac content and superoxide production of these cells in dose-dependent manner.
-
(1992)
J Biol Chem
, vol.267
, pp. 20540-20542
-
-
Dorseuil1
Vazquez2
Lang3
Bertoglio4
Gacon5
Leca6
-
23
-
-
0026666665
-
The Translocation of Respiratory Burst Oxidase Components from Cytosol to Plasma Membrane is Regulated by Guanine Nucleotides and Diacylglycerol
-
of special interest, This paper describes the GTP and diacylglycerol dependency of the p47-phox and p67-phox translocation during activation with SDS in a cell-free assay system.
-
(1992)
J Biol Chem
, vol.267
, pp. 19901-19906
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-
Park1
Babior2
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24
-
-
0027418759
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The Respiratory Burst Oxidase of Human Neutrophils. Guanine Nucleotides and Arachidonate Regulate the Assembly of a Multicomponent Complex in a Semirecombinant Cell-Free system
-
558, the role of a GTP-binding protein in translocation and/or complex formation of p47-phox and p67-phox with cytochrome b.
-
(1993)
J Biol Chem
, vol.268
, pp. 8624-8631
-
-
Uhlinger1
Tyagi2
Inge3
Lambeth4
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25
-
-
0026520888
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Isoprenoid Metabolism is Required For Stimulation of the Respiratory Burst Oxidase of HL-60 cells
-
(1992)
J Clin Invest
, vol.89
, pp. 402-408
-
-
Bokoch1
Prossnitz2
-
26
-
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0027537491
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Carboxyl Methylation of Ras-Related Proteins During Signal Transduction in Neutrophils
-
of outstanding interest, This paper presents evidence for an important role of carboxyl methylation in neutrophil responses to the chemoattractant fMLP and in Rac2 translocation to the plasma membrane.
-
(1993)
Science
, vol.259
, pp. 977-980
-
-
Philips1
Pillinger2
Staud3
Volker4
Rosenfeld5
Weissman6
Stock7
-
27
-
-
0027442667
-
Biologically Active Lipids are Regulators of Rac: GDI Complexation
-
of outstanding interest, This paper investigates cytosolic Rac/[Rho]GDI complexes in neutrophils and the ability of physiological lipid second messengers and activators of the NADPH oxidase to disrupt this interaction.
-
(1993)
J Biol Chem
, vol.268
, pp. 26206-26211
-
-
Chuang1
Bohl2
Bokoch3
-
28
-
-
0027393663
-
GDP Dissociation Inhibitor Prevents Intrinsic and GTPase Activating Protein-Stimulated GTP Hydrolysis by the Rac GTP-Binding Protein
-
of outstanding interest, The authors describe the inhibition of intrinsic as well as GAP-mediated GTP hydrolysis on Rac by [Rho]GDI. [Rho]GDI was also found to affect GTPγS dissociation, thereby indicating an interaction with the GTP-bound form of Rac proteins.
-
(1993)
J Biol Chem
, vol.268
, pp. 775-778
-
-
Chuang1
Xu2
Knaus3
Hart4
Bokoch5
-
29
-
-
0027232261
-
A Novel Role for RhoGDI as an Inhibitor of GAP Proteins
-
of outstanding interest, An examination of the role of post-translational modifications of Rac1 and RhoA in binding to [Rho]GDI. [Rho]GDI was shown to form stable complexes with Rac and Rho in both the GDP- and GTP-bound states, and the latter was resistant to the action of GAPs.
-
(1993)
EMBO J
, vol.12
, pp. 1915-1921
-
-
Hancock1
Hall2
-
30
-
-
0027102910
-
Copurification of Rho Protein and the Rho-GDP Dissociation Inhibitor from Bovine Neutrophil Cytosol. Effect of Phosphoinositides on Rho ADP-Ribosylation by the C3 Exoenzyme of Clostridium botulinium
-
of outstanding interest, Purification and identification of a Rho/[Rho]GDI complex from bovine neutrophil cytosol using ADP-ribosylation assays for detection.
-
(1992)
Biochemistry
, vol.31
, pp. 12863-12869
-
-
Bourmeyster1
Stasia2
Garin3
Gagon4
Boquet5
Vignais6
-
33
-
-
0027163225
-
Activation of Neutrophil Leukocytes: Chemoattractant Receptors and Respiratory Burst
-
of special interest, Recent review about leukocyte stimulation, presenting insights in the structure of chemoattractant receptors and discussing the role of nucleotides, lipids and phosphatases on NADPH oxidase regulation.
-
(1993)
FASEB J
, vol.7
, pp. 1004-1010
-
-
Baggiolini1
Boulay2
Badwey3
Curnutte4
-
34
-
-
0027526303
-
cDNA Cloning of a Human mRNA Preferentially Expressed in Hematopoietic Cells and With Homology to a GDP-Dissociation Inhibitor for the Rho GTP-Binding Proteins
-
of special interest, Identification of the mRNA for the human D4 gene, which encodes a 23 kDa protein sharing 67% amino acid identity with [Rho]GDI. Expression levels of D4 appear to be developmentally regulated in hematopoietic cell lines.
-
(1993)
Proc Natl Acad Sci USA
, vol.90
, pp. 1479-1483
-
-
Lelias1
Adra2
Wulf3
Guillemot4
Khagad5
Caput6
Lim7
-
35
-
-
0028009756
-
The non-catalytic domain of ras-GAP inhibits transformation induced by G protein coupled receptors.
-
of special interest, in press, This paper characterizes the increased affinity of a Rac2 mutant for both GAP binding and NADPH oxidase activation. Comparison of stable GTP versus GDP bound forms of Rac2 showed that only the GTPbound proteins were active.
-
(1994)
Oncogene
-
-
Xu1
Barry2
Chuang3
Settleman4
Bokoch5
-
36
-
-
0027132009
-
Regulation of NADPH Oxidase Activity by Rac GTPase Activating Protein(s)
-
of special interest, This study illustrates the ability of Rac GAPs to inhibit activation of the NADPH oxidase in a cell-free system when added before initiating stimulus.
-
(1993)
Mol Biol Cell
, vol.4
, pp. 1217-1223
-
-
Heyworth1
Knaus2
Settleman3
Curnutte4
Bokoch5
-
38
-
-
0026778133
-
The Small GTP-Binding Protein Rho Regulates the Assembly of Focal Adhesion and Actin Stress Fibers in Response to Growth Factors
-
(1992)
Cell
, vol.70
, pp. 389-399
-
-
Ridley1
Hall2
-
39
-
-
0027232605
-
Emerging Concepts in the Ras Superfamily of GTP-Binding Proteins
-
of special interest, Overview of recent developments in the Ras superfamily of GTP-binding proteins, discussing structure-function relationships, regulatory proteins for the GDP/GTP cycle, and their role in cellular processes.
-
(1993)
FASEB J
, vol.7
, pp. 750-759
-
-
Bokoch1
Der2
-
40
-
-
0027391509
-
Involvement of rho p21 and Its Inhibitory GDP/GTP Exchange Protein (rho GDI) in Cell Motility
-
of outstanding interest, The effects of Rho, [Rho]GDI and smgGDS on the actomyosin system involved in fibroblast motility are examined. The inhibition of [Rho]GDI was overcome by co-injection of GTP-bound Rho, suggesting that [Rho]GDI could mask the effector-binding region.
-
(1993)
Mol Cell Biol
, vol.13
, pp. 72-79
-
-
Takaishi1
Kikuchi2
Kuroda3
Kotani4
Sasaki5
Takai6
-
43
-
-
0027238929
-
Stimulation of Human Neutrophils with fMLP Induces Tyrosine Phosphorylation and Activation of Two Distinct Mitogen-Activated Protein Kinases
-
of special interest, This study demonstrates the phosphorylation and activation of two forms of MAP kinase in chemoattractant-stimulated human neutrophils. These findings raise the possibility that activation of the MAP kinase cascade might play a role in the acute regulation of phagocyte activation.
-
(1993)
J Immunol
, vol.150
, pp. 1563-1578
-
-
Torres1
Hall2
O'Neill3
|