inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis

Science

Volumn 263, Issue 5144, 1994, Pages 227-230

  Banerjee, Asesh ^a   Dubnau, Eugenie ^a   Quemard, Annaik ^a   Balasubramanian, V ^a   Um, Kyung Sun ^a   Wilson, Theresa ^b   Collins, Des ^b   De Lisle, Geoffrey ^b   Jacobs Jr , William R ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b AGRESEARCH   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

ETHIONAMIDE; ISONIAZID;

ANTIBIOTIC RESISTANCE; ARTICLE; MISSENSE MUTATION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL;

EID: 0028156861     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.8284673     Document Type: Article

References (57)

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40. The inhA DNA sequences have been submitted to GenBank. The accession numbers are U02530 (for M. smegmatis) and U02492 (for M. tuberculosis). The M. bovis sequence is identical to that of M. tuberculosis.
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42. 2651 (MIC = 50 μg/ml).
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52. Abbreviations for the amino acid residues are: A Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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56. 2155 (28) bearing the indicated plasmids were grown in 7H9 broth containing kanamycin (15 μg/ml), and dilutions were plated on 7H10 agar plates containing kanamycin alone or kanamycin with various concentrations of INH or ETH. The strains without any plasmid were grown in 7H9 broth, and dilutions were plated on 7H10 agar plates and on 7H10 agar plates with various concentrations of INH or ETH.
57. We thank B. Bloom, J. Blanchard, J. Sacchettini, and D. Dubnau for helpful discussions; L. Pascopella for providing mycobacterial libraries; and M. Avelle and R. Williams for technical assistance. Supported by National Institutes of Health grant AI27160 (W.R.J.), National Cooperative Drug Discovery group grant UO1AI30189 (W.R.J.), and New Zealand FRST grant C10306 (D.C.).