Applications of interaction traps/two-hybrid systems to biotechnology research

Current Opinion in Biotechnology

Volumn 5, Issue 5, 1994, Pages 482-486

  Mendelsohn, Andrew R ^a   Brent, Roger ^b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA BINDING PROTEIN; RECOMBINANT DNA;

BIOTECHNOLOGY; DNA RECOMBINATION; GENE ACTIVATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN ISOLATION; PROTEIN PROTEIN INTERACTION; RECOMBINANT DNA TECHNOLOGY; REPORTER GENE; SHORT SURVEY; TRANSCRIPTION REGULATION; YEAST;

EID: 0028148239     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/0958-1669(94)90061-2     Document Type: Article

References (39)

1. A Eukaryotic Transcriptional Activator Bearing the DNA Specificity of a Prokaryotic Repressor
2. Separation of the DNA Binding from the Transcription-Activating Function of a Eukaryotic Regulatory Protein
3. A New Class of Transcriptional Activators
4. Converting a Eukaryotic Transcriptional Inhibitor into an Activator
5. Functional Dissection of VP16, the Trans-Activator of Herpes Simplex Virus Immediate Early Gene Expression
6. Evidence of DNA: Protein Interactions that Mediate HSV-1 Immediate Early Gene Activation by VP16
7. A Novel Genetic System to Detect Protein-Protein Interactions
8. The Two-Hybrid System: A Method to Identify and Clone Genes for Proteins that Interact with a Protein of Interest
9. The Retinoblastoma Protein Associates with the Protein Phosphatase Type 1 Catalytic Subunit
10. Cdi1, a Human G1 and S Phase Protein Phosphatase that Associates with Cdk2
11. Mammalian Ras Interacts Directly with the Serine/Threonine Kinase Raf
12. The p21 Cdk-Interacting Protein Cip1 is a Potent Inhibitor of G1 Cyclin-Dependent Kinases
13. Mxi1, a Protein that Specifically Interacts with Max to Bind Myc-Max Recognition Sites
14. Fused protein domains inhibit DNA binding by LexA.
15. LexA is a Repressor of the Colicin El Gene
16. Regulation and Autoregulation by LexA Protein
17. The SOS Regulatory System of Escherichia coli
18. Large-Scale Purification, Oligomerization Equilibria, and Specific Interaction of the LexA Repressor of Escherica coli
19. Repression of the E. coli recA Gene Requires at Least Two LexA Protein Monomers
20. A Bacterial Repressor Protein or a Yeast Transcriptional Terminator Can Block Upstream Activation of a Yeast Gene
21. DNA Binding is Not Sufficient for Nuclear Localization of Regulatory Proteins in Saccharomyces cerevisiae
22. Separation of the DNA Binding from the Transcription-Activating Function of a Eukaryotic Regulatory Protein
23. Amino Terminus of the Yeast GAL4 Gene Product is Sufficient for Nuclear Localization
24. An Amino-Terminal Fragment of GAL4 Binds DNA as a Dimer
25. GAL4 Derivatives Function Alone and Synergistically with Mammalian Activators in Vitro
26. Negative Effect of the Transcriptional Activator GAL4
27. Selective Inhibition of Activated but Not Basal Transcription by the Acidic Activation Domain of VP16: Evidence for Transcriptional Adaptors
28. Karyoplasmic Interaction Selection Strategy: A General Strategy to Detect Protein-Protein Interactions in Mammalian Cells
29. A Contingent Replication Assay for the Detection of Protein-Protein Interactions in Animal Cells
30. A RAP1-Interacting Protein Involved in Transcriptional Silencing and Telomere Length Regulation
31. Green Fluorescent Protein as a Marker for Gene Expression
32. Detecting LacZ Gene Expression in Living Cells with New Lipophilic, Fluorogenic β-Galactosidase Substrates
33. Effects of Polymixin B Sulfate and Polymixin B Nonapeptide on Growth and Permeability ot the Yeast Saccharomyces cerevisiae
34. Thymidine Utilization by tut Mutants and Facile Cloning of Mtant Alleles by Plasmid Conversion in S. cerevisiae
35. Transformation of Yeast
36. Interaction mating reveals binary and ternary connections between Drosophila cell cycle regulators.
37. Fishing for Partners
38. The Two-Hybrid System: An Assay for Protein-Protein Interactions