Analysis of the p53 Gene and Its Expression in Human Glioblastoma Cells

Cancer Research

Volumn 54, Issue 3, 1994, Pages 649-652

  Van Meir, Erwin G ^a,b   Kikuchi, Tetsuro ^a   Tada, Mitsuhiro ^a   Li, Hong ^a   Diserens, Annie Claire ^a   Wojcik, Brian E ^b   Su Huang, H J ^b   Friedmann, Theodore ^b   de Tribolet, Nicolas ^a   Cavenee, Webster K ^b  

^a LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P53;

ARTICLE; CANCER CELL CULTURE; CANCER GROWTH; CHROMOSOME 17P; GENE EXPRESSION; GENE SEQUENCE; GLIOBLASTOMA; HETEROZYGOSITY; POINT MUTATION; PRIORITY JOURNAL; PROTEIN SYNTHESIS; REPORTER GENE;

ANIMALS; BASE SEQUENCE; BLOTTING, NORTHERN; BRAIN NEOPLASMS; FEMALE; GENE EXPRESSION; GENES, P53; GLIOBLASTOMA; HUMANS; MICE; MICE, NUDE; NEOPLASM TRANSPLANTATION; PROMOTER REGIONS (GENETICS); RNA, NEOPLASM; TRANS-ACTIVATION (GENETICS); TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0028117269     PISSN: 00085472     EISSN: 15387445     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Russell, D. S., and Rubinstein, L. J. Pathology of tumours of the nervous system. London: Edward Arnold, 1989.
2. Fearon, E. R., and Vogelstein, B. A genetic model for colorectal tumorigenesis. Cell, 61: 759-767, 1990.
3. Cavenee, W. K., Scrable, H. J., and James, C. D. Molecular genetics of human cancer predisposition and progression. Mutat. Res., 247: 199-202, 1991.
4. Louis, D. N., von Deimling, A., Chung, R. Y., Rubio, M. P., Whaley, J. M., Eibl, R. H., Ohgaki, H., Wiestler, O. D., Thor, A. D., and Seizinger, B. R. Comparative study of p53 gene and protein alterations in human astrocytic tumors. J. Neuropathol. Exp. Neurol., 52: 31-38, 1993.
5. Reifenberger, G., Liu, L., Ichimura, K., Schmidt, E. E., and Collins, V. P. Amplification and overexpression of the MDM2 gene in a subset of human malignant gliomas without p53 mutations. Cancer Res., 53: 2736-2739, 1993.
6. Hayashi, Y., Yamashita, J., and Yamaguchi, K. Timing and role of p53 gene mutation in the recurrence of glioma. Biochem. Biophys. Res. Commun., 180: 1145-1150, 1991.
7. Sidransky, D., Mikkelsen, T., Schwechheimer, K., Rosenblum, M. L., Cavenee, W. K., and Vogelstein, B. Clonal expansion of p53 mutant cells is associated with brain tumour progression. Nature (Lond.), 355: 846-847, 1992.
8. Van Meir, E., Ceska, M., Effenberger, F., Walz, A., Grouzmann, E., Desbaillets, I., Frei, K., Fontana, A., and de Tribolet, N. Interleukin-8 is produced in neoplastic and infectious diseases of the human central nervous system. Cancer Res., 52: 4297-4305, 1992.
9. Van Meir, E., Sawamura, Y., Diserens, A. C., Hamou, M. F., and de Tribolet, N. Human glioblastoma cells release interleukin 6 in vivo and in vitro. Cancer Res., 50: 6683-6688, 1990.
10. Kikuchi, T., Rempel, S. A., Rutz, H. P., de Tribolet, N., Mulligan, L., Cavenee, W. K., Jothy, S., Leduy, L., and Van Meir, E. Turcot's syndrome of glioma and polyposis occurs in the absence of germ line mutations of exons 5 to 9 of the p53 gene. Cancer Res., 53: 957-961, 1993.
11. Kern, S. E., Pietenpol, J. A., Thiagalingam, S., Seymour, A., Kinzler, K. W., and Vogelstein, B. Oncogenic forms of p53 inhibit p53-regulated gene expression. Science (Washington DC), 256: 827-830, 1992.
12. Graham, F. L., and Van der Eb, A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology, 52: 456-467, 1973.
13. Seed, B., and Sheen, J. Y. A simple phase-extraction assay for chloramphenicol acetyltransferase activity. Gene, 67: 271-277, 1988.
14. Ullrich, S. J., Mercer, W. E., and Appella, E. Human wild-type p53 adopts a unique conformational and phosphorylation state in vivo during growth arrest of glioblastoma cells. Oncogene, 7: 1635-1643, 1992.
15. Godbout, R., Miyakoshi, J., Dobler, K. D., Andison, R., Matsuo, K., Allalunis, T. M., Takebe, H., and Day, R. H. I. Lack of expression of tumor-suppressor genes in human malignant glioma cell lines. Oncogene, 7: 1879-1884, 1992.
16. Nigro, J. M., Baker, S. J., Preisinger, A. C., Jessup, J. M., Hostetter, R., Cleary, K., Bigner, S. H., Davidson, N., Baylin, S., Devilee, P., et al. Mutations in the p53 gene occur in diverse human tumour types. Nature (Lond.), 342: 705-708, 1989.
17. Rubio, M. P., von Deimling, A., Yandell, D. W., Wiestler, O. D., Gusella, J. E., and Louis, D. N. Accumulation of wild type p53 protein in human astrocytomas. Cancer Res., 53: 3465-3467, 1993.
18. Vogelstein, B., and Kinzler, K. W. p53 function and dysfunction. Cell, 70: 523-526, 1992.
19. Bigner, D. D., Bigner, S. H., Ponton, J., Westermark, B., Mahaley, M. S., Ruoslahti, E., Herschman, H., Eng, L. F., and Wikstrand, C. J. Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas. J. Neuropathol. Exp. Neurol., 40: 201-229, 1981.
20. Casey, G., Lo, H. M., Lopez, M. E., Vogelstein, B., and Stanbridge, E. J. Growth suppression of human breast cancer cells by the introduction of a wild-type p53 gene. Oncogene, 6: 1791-1797, 1991.
21. Cajot, J. F., Anderson, M. J., Lehman, T. A., Shapiro, H., Briggs, A. A., and Stan-bridge, E. J. Growth suppression mediated by transfection of p53 in Hut292DM human lung cancer cells expressing endogenous wild-type p53 protein. Cancer Res., 52: 6956-6960, 1992.
22. Lehman, T. A., Bennett, W. P., Metcalf, R. A., Welsh, J. A., Ecker, J., Modali, R. V., Ullrich, S., Romano, J. W., Appella, E., Testa, J. R., et al. p53 mutations, ras mutations, and p53-heat shock 70 protein complexes in human lung carcinoma cell lines. Cancer Res., 51: 4090-4096, 1991.
23. DavidofI, A. M., Pence, J. C., Shorter, N. A., Iglehart, J. D., and Marks, J. R. Expression of p53 in human neuroblastoma- and neuroepithelioma-derived cell lines. Oncogene, 7: 127-133, 1992.
24. Saylors, R. B., Sidransky, D., Friedman, H. S., Bigner, S. H., Bigner, D. D., Vogelstein, B., and Brodeur, G. M. Infrequent p53 gene mutations in medulloblastomas. Cancer Res., 51: 4721-4723, 1991.
25. Lu, X., Park, S. H., Thompson, T. C., and Lane, D. P. /tar-induced hyperplasia occurs with mutation of p53y but activated ras and myc together can induce carcinoma without p53 mutation. Cell, 70: 153-161, 1992.
26. Ionov, Y., Peinado, M. A., Malkhosyan, S., Shibata, D., and Perucho, M. Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis. Nature (Lond.), 363: 558-561, 1993.
27. Mercer, W. E., Shields, M. T., Amin, M., Sauve, G. J., Appella, E., Romano, J. W., and Ullrich, S. J. Negative growth regulation in a glioblastoma tumor cell line that conditionally expresses human wild-type p53. Proc. Natl. Acad. Sci. USA, 87: 6166-6170, 1990.
28. Tabuchi, K., Fukuyama, K., and Mineta, T. Cytogenetic diagnosis of brain tumors. Brain & Nerve, 44: 871-879, 1992.