Factors affecting sensitivity to EO9 in rodent and human tumour cells in vitro: DT-diaphorase activity and hypoxia

European Journal of Cancer

Volumn 30, Issue 7, 1994, Pages 1013-1019

  Robertson, N ^a   Haigh, A ^a   Adams, G E ^a   Stratford, I J ^a  

^a MEDICAL RESEARCH COUNCIL   (United Kingdom)

Author keywords

bioreductive drugs; DT diaphorase; EO9; hypoxia

Indexed keywords

5 (1 AZIRIDINYL) 3 HYDROXYMETHYL 2 (3 HYDROXY 1 PROPENYL) 1 METHYL 4,7 INDOLEDIONE; ANTINEOPLASTIC AGENT; DICOUMAROL; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) DEHYDROGENASE (QUINONE);

ANIMAL CELL; ARTICLE; CANCER; CANCER CELL CULTURE; CONTROLLED STUDY; DRUG ACTIVITY; ELECTROCARDIOGRAM; HUMAN; HUMAN CELL; HYPOXIA; MOUSE; NONHUMAN; PHARMACOLOGY; PRIORITY JOURNAL;

ANIMAL; ANOXIA; ANTINEOPLASTIC AGENTS; AZIRIDINES; BREAST NEOPLASMS; COLONIC NEOPLASMS; HAMSTERS; HUMAN; INDOLES; LUNG NEOPLASMS; MAMMARY NEOPLASMS; NAD(P)H DEHYDROGENASE (QUINONE); OXYGEN; SUPPORT, U.S. GOV'T, P.H.S.; TUMOR CELLS, CULTURED;

EID: 0028101349     PISSN: 09598049     EISSN: None     Source Type: Journal    
DOI: 10.1016/0959-8049(94)90134-1     Document Type: Article

References (35)

1. Mitomycin analogs I. Indoloquinones as (potential) bisalkylating agents
2. EO9: a novel bioreductive alkylating indoloquinone with preferential solid tumour activity and lack of bone marrow toxicity in preclinical models
3. In vitro activity of the novel indoloquinone EO9 and the influence of pH on cytotoxicity
4. Metabolism of mitomycin C by DT-diaphorase: role in mitomycin C-induced DNA damage and cytotoxicity in human colon carcinoma cell
5. DT-diaphorase and cancer chemotherapy
6. The role of NAD(P)H: quinone reductase (EC1.6.99.2,DT-diaphorase) in the reductive bioactivation of the novel indoloquinone antitumour agent EO9
7. The sensitivity of human tumour cells to quinone bioreductive drugs: what role for DT-diaphorase?
8. Enzyme-directed bioreductive drug development
9. DT-diaphorase activity correlates with sensitivity to the indoloquinone EO9 in mouse and human colon carcinomas
10. Bioreductive drugs as post-irradiation sensitizers: comparison of dual function agents with SR 4233 and the mitomycin C analogue EO9
11. Induction of tumour hypoxia post-irradiation: a methd for increasing the sensitizing efficiency of misonidazole and RSU 1069 in vivo
12. Dual-function 2-nitroimidazoles as hypoxic cell radiosensitizers and bioreductive cytotoxins: in vivo evaluation in KHT murine sarcomas
13. SR4233: a new bioreductive agent with high selective toxicity for hypoxic mammalian cells
14. Bioreductive drugs in cancer therapy
15. Bioreductive drugs—dual function agents and related compounds
16. Isolation and cross-sensitivity of X-ray-sensitive mutants of V79-4 hamster cells
17. The effects of three bioreductive drugs (mitomycin C, RSU-1069 and SR4233) on cell lines selected for their sensitivity to mitomycin C or ionising radiation
18. Decreased NADPH: cytochrome P-450 reductase activity and impaired drug activation in a mammalian cell line resistant to mitomycin C under aerobic but not hypoxic conditions
19. Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions
20. Isolation of Chinese hamster ovary cell mutants deficient in excision repair and mitomycin C bioactivation
21. Chinese hamster ovary cell lines resistant to mitomycin C under aerobic but not hypoxic conditions are deficient in DT diaphorase
22. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
23. Evaluation of a tetrazolium-based semi-automated colorimetric assay: assessment of chemosensitivity testing
24. The assessment of bioreductive drug toxicity in vitro and in experimental tumours in vivo
25. Mutant rodent cell lines sensitive to ultraviolet light, ionizing radiation and cross-linking agents: a comprehensive survey of genetic and biochemical characteristics
26. Elevated DT-diaphorase activity and messenger RNA content in human non-small cell lung carcinoma: relationship to the response of lung tumour xenografts to mitomycin C
27. DT-diaphorase questionable role in mitomycin C resistance, but a target for novel bioreductive drugs?
28. A note on the inhibition of DT-diaphorase by dicoumarol
29. Bioreductive activation of quinones: redox properties and thiol reactivity
30. Reductive activation of mitomycin C
31. Unusually marked hypoxic sensitisation to indoloquinone EO9 and mitomycin C in a human colon tumour cell lines that lacks DT-diaphorase activity
32. Quinone bioreductive drugs: interplay between hypoxia and reductive enzymology for activation and toxicity
33. Free radical generation following reduction of EO9: involvement in cytotoxicity
34. Structure-activity relationships for DT-diaphorase reduction of hypoxic cell directed agents: indoloquinones and diaziridinyl benzoquinones
35. Effect of deficiencies in DNA repair on the toxicity of mitomycin C and porfiromycin to CHO cells under aerobic and hypoxic conditions