New in vitro fluorimetric microtitration assays for toxicological screening of drugs

Cell Biology and Toxicology

Volumn 10, Issue 5-6, 1994, Pages 329-337

  Rat, P ^a,b   Korwin Zmijowska, C ^a   Warnet, J M ^b   Adolphe, M ^a  

^a PSL RESEARCH UNIVERSITY   (France)

^b UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

cold light fluorimetry; cytotoxicity assays classification; fluorescent Neutral Red; microtitration assays; Rhodamine 123; UV

Indexed keywords

4 [5 (4 METHYL 1 PIPERAZINYL)[2,5' BI 1H BENZIMIDAZOL] 2' YL]PHENOL; CALCEIN; CARBON TETRACHLORIDE; CYTOTOXIC AGENT; NEUTRAL RED; RHODAMINE 123;

ARTICLE; CELL ADHESION; CELL DAMAGE; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CYTOFLUOROMETRY; CYTOTOXICITY; DRUG SCREENING; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MICROFLUOROMETRY; MITOCHONDRIAL RESPIRATION; PRIORITY JOURNAL; TITRIMETRY; TOXICITY TESTING; ULTRAVIOLET A RADIATION;

CARBON TETRACHLORIDE; CELL ADHESION; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; DRUG EVALUATION, PRECLINICAL; FLUORESCENT DYES; FLUOROMETRY; HUMAN; MITOCHONDRIA; TITRIMETRY; TOXICITY TESTS; ULTRAVIOLET RAYS;

EID: 0028073912     PISSN: 07422091     EISSN: 15736822     Source Type: Journal    
DOI: 10.1007/BF00755779     Document Type: Article

References (31)

1. Evaluation of Rhodamine 123 as a fluorescent probe to assess mitochondrial damage due to cytotoxic drugs
2. Comments on the scientific validation and regulatory acceptance of in vitro toxicity tests
3. Basal cytotoxicity and target organ toxicity in risk assessment
4. Correlation and validation of alternative methods to the Draize eye irritation test (OPAL project)
5. A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90)
6. Rapid chemosensitivity assay with human normal and tumor cells in vitro
7. New fluorimetric microtitration assay for the quantification of cell-cell adherence
8. Probing mitochondria in living cells with Rhodamine 123
9. Comparative assessment of in vitro toxicity of xenobiotics using flow cytometry and spectrophotometry
10. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability
11. A double in situ cytotoxicity assay using the fluorescent probes Neutral Red and BCECF-AM
12. A multicentre study of acute in vitro cytotoxicity in rat liver cells
13. On the permeability to lissamine green and other dyes in the course of cell injury and cell death
14. Localization of mitochondria in living cells with Rhodamine 123
15. Estimation of cell number by Neutral Red content: Applications for proliferative and survival assays
16. Is the estimation of the activity of MTT-reduction suitable for determination of the basal cytotoxicity
17. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays
18. Hepatocytes in primary culture: an alternative to LD 50 testing? Validation of a predictive model by multivariate analysis
19. Hoechst 33342 for microfluorimetric measurement of adrenocortical tumor cell proliferation
20. DNA fluorimetric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water
21. Fluorimetric DNA assay for cell growth estimation
22. Assessment of mitochondrial membrane potential as an indicator of cytotoxicity
23. Cell injury and antidote evaluations with fluorimetric microtitration assays
24. New microtitration fluorometric technology — applications to dermotoxicity
25. Measurements of cell proliferation in microculture using Hoechst 33342 for rapid semiautomated microfluorimetric determination of chromatin DNA
26. Mitochondrial analysis in living cells: the use of rhodamine 123 and flow cytometry
27. Tetrazolium-based assays for cellular viability — a critical examination of selected parameters affecting formazan production
28. New fluorescent dyes for lymphocytes migration studies. Analysis by flow cytometry and fluorescence microscopy
29. Relationships between the mitochondrial transmembrane potential, ATP concentration and cytotoxicity in isolated rat hepatocytes