Detection of Borrelia burgdorferi DNA by Polymerase Chain Reaction in Synovial Fluid from Patients with Lyme Arthritis

New England Journal of Medicine

Volumn 330, Issue 4, 1994, Pages 229-234

  Nocton, James J ^a   Dressler, Frank ^a   Rutledge, Barbara J ^b   Rys, Paul N ^b   Persing, David H ^b   Steere, Allen C ^a  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b MAYO CLINIC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ADULT; AGED; ANTIBIOTIC THERAPY; ARTICLE; BACTERIUM DETECTION; BORRELIA BURGDORFERI; CONTROLLED STUDY; DISEASE COURSE; DNA DETERMINATION; FEMALE; HUMAN; LYME DISEASE; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SYNOVIAL FLUID;

ADOLESCENT; ADULT; AGED; ARTHRITIS, INFECTIOUS; BASE SEQUENCE; BORRELIA BURGDORFERI GROUP; CHILD; CHILD, PRESCHOOL; DNA PRIMERS; DNA PROBES; DNA, BACTERIAL; FEMALE; HUMANS; LYME DISEASE; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SYNOVIAL FLUID;

EID: 0028057617     PISSN: 00284793     EISSN: 15334406     Source Type: Journal    
DOI: 10.1056/NEJM199401273300401     Document Type: Article

References (44)

1. Steere AC Lyme disease. N Engl J Med 1989; 321: 586-596
2. Steere AC, Schoen RT, Taylor E The clinical evolution of Lyme arthritis. Ann Intern Med 1987; 107: 725-731
3. Steere AC, Gibofsky A, Patarroyo ME, Winchester RJ, Hardin JA, Malawista SE Chronic Lyme arthritis: clinical and immunogenetic differentiation from rheumatoid arthritis. Ann Intern Med 1979; 90: 896-901
4. Szer IS, Taylor E, Steere AC The long-term course of Lyme arthritis in children. N Engl J Med 1991; 325: 159-163
5. Steere AC, Green J, Schoen RT, Successful parenteral penicillin therapy of established Lyme arthritis. N Engl J Med 1985; 312: 869-874
6. Dattwyler RJ, Halperin JJ, Volkman DJ, Luft BJ Treatment of late Lyme borreliosis -- randomised comparison of ceftriaxone and penicillin. Lancet 1988; 1: 1191-1194
7. Steere AC, Levin RE, Molloy PJ, Antibiotic treatment of Lyme arthritis. Arthritis Rheum 1993; 36: Suppl: S40-S40 abstract.
8. Steere AC, Dwyer E, Winchester R Association of chronic Lyme arthritis with HLA-DR4 and HLA-DR2 alleles. N Engl J Med 1990; 323: 219-223
9. Kalish RA, Leong JM, Steere AC Association of treatment-resistant chronic Lyme arthritis with HLA-DR4 and antibody reactivity to OspA and OspB of Borrelia burgdorferi. Infect Immun 1993; 61: 2774-2779
10. Berger BW, Kaplan MH, Rothenberg IR, Barbour AG Isolation and characterization of the Lyme disease spirochete from the skin of patients with erythema chronicum migans. J Am Acad Dermatol 1985; 13: 444-449
11. Snydman DR, Schenkein DP, Berardi VP, Lastavica CC, Pariser KM Borrelia burgdorferi in joint fluid in chronic Lyme arthritis. Ann Intern Med 1986; 104: 798-800
12. Schmidli J, Hunziker T, Moesli P, Schaad UB Cultivation of Borrelia burgdorferi from joint fluid three months after treatment of facial palsy due to Lyme borreliosis. J Infect Dis 1988; 158: 905-906
13. Steere AC, Duray PH, Butcher EC Spirochetal antigens and lymphoid cell surface markers in Lyme synovitis: comparison with rheumatoid synovium and tonsillar lymphoid tissue. Arthritis Rheum 1988; 31: 487-495
14. Rosa PA, Schwan TG A specific and sensitive assay for the Lyme disease spirochete Borrelia burgdorferi using the polymerase chain reaction. J Infect Dis 1989; 160: 1018-1029
15. Nielsen SL, Young KKY, Barbour AG Detection of Borrelia burgdorferi DNA by the polymerase chain reaction. Mol Cell Probes 1990; 4: 73-79
16. Persing DH, Telford SR III, Spielman A, Barthold SW Detection of Borrelia burgdorferi infection in Ixodes dammini ticks with the polymerase chain reaction. J Clin Microbiol 1990; 28: 566-572
17. Persing DH, Telford SR III, Rys PN, Detection of Borrelia burgdorferi DNA in museum specimens of Ixodes dammini ticks. Science 1990; 249: 1420-1423
18. Malloy DC, Nauman RK, Paxton H Detection of Borrelia burgdorferi using the polymerase chain reaction. J Clin Microbiol 1990; 28: 1089-1093
19. Lebech AM, Hindersson P, Vuust J, Hansen K Comparison of in vitro culture and polymerase chain reaction for detection of Borrelia burgdorferi in tissue from experimentally infected animals. J Clin Microbiol 1991; 29: 731-737
20. Guy EC, Stanek G Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction. J Clin Pathol 1991; 44: 610-611
21. Williams WV, Callegari P, Freundlich B, Molecular diagnosis of Borrelia burgdorferi infection (Lyme disease). DNA Cell Biol 1992; 11: 207-213
22. Kruger WH, Pulz M Detection of Borrelia burgdorferi in cerebrospinal fluid by the polymerase chain reaction. J Med Microbiol 1991; 35: 98-102
23. Jaulhac B, Nicolini P, Piemont Y, Monteil H Detection of Borrelia burgdorferi in cerebrospinal fluid of patients with Lyme borreliosis. N Engl J Med 1991; 324: 1440-1440
24. Keller TL, Halperin JJ, Whitman M PCR detection of Borrelia burgdorferi DNA in cerebrospinal fluid of Lyme neuroborreliosis patients. Neurology 1992; 42: 32-42
25. Lebech A, Hansen K Detection of Borrelia burgdorferi DNA in urine samples and cerebrospinal fluid samples from patients with early and late Lyme neuroborreliosis by polymerase chain reaction. J Clin Microbiol 1992; 30: 1646-1653
26. Nielsen SL, Peter JB B. burgdorferi persistently detected by polymerase chain reaction in synovial fluid of a patient with Lyme arthritis resistant to therapy. Clin Res 1990; 38: 402A-402A abstract.
27. Malawista SE, Schoen RT, Moore TL, Dodge DE, White TJ, Persing DH Failure of multitarget detection of Borrelia burgdorferi-associated DNA sequences in synovial fluids of patients with juvenile rheumatoid arthritis: a cautionary note. Arthritis Rheum 1992; 35: 246-247
28. Goodman JL, Jurkovich P, Kramber JM, Johnson RC Molecular detection of persistent Borrelia burgdorferi in the urine of patients with active Lyme disease. Infect Immun 1991; 59: 269-278
29. Melchers W, Meis J, Rosa P, Amplification of Borrelia burgdorferi DNA in skin biopsies from patients with Lyme disease. J Clin Microbiol 1991; 29: 2401-2406
30. Rabb DC, Lesher JL Jr, Chandler FW Polymerase chain reaction confirmation of Borrelia burgdorferi in benign lymphocytic infiltrate of dermis. J Am Acad Dermatol 1992; 26: 267-268
31. Schwartz I, Wormser GP, Schwartz JJ, Diagnosis of early Lyme disease by polymerase chain reaction amplification and culture of skin biopsies from erythema migrans lesions. J Clin Microbiol 1992; 30: 3082-3088
32. Liebling MR, Nishio MJ, Rodriguez A, Sigal LH, Jin T, Louie JS The polymerase chain reaction for the detection of Borrelia burgdorferi in human body fluids. Arthritis Rheum 1993; 36: 665-675
33. Luft BJ, Steinman CR, Neimark HC, Invasion of the central nervous system by Borrelia burgdorferi in acute disseminated infection. JAMA 1992; 267: 1364-1367 [Erratum, JAMA 1992;268:872.]
34. Holodniy M, Kim S, Katzenstein D, Konrad M, Groves E, Merigan TC Inhibition of human immunodeficiency virus gene amplification by heparin. J Clin Microbiol 1991; 29: 676-679
35. Persing DH, Rutledge BJ, Rys PN, Target imbalance: disparity of Borrelia burgdorferi genetic material in synovial fluid from Lyme arthritis patients. J Infect Dis 1994; 169: 664-668
36. Cimino GD, Metchette KC, Tessman JW, Hearst JE, Isaacs ST Post-PCR sterilization: a method to control carryover contamination for the polymerase chain reaction. Nucleic Acids Res 1991; 19: 99-107
37. Persing DH, Mathiesen D, Marshall WF, Detection of Babesia microti by polymerase chain reaction. J Clin Microbiol 1992; 30: 2097-2103
38. Rahn DW, Malawista SE Lyme disease: recommendations for diagnosis and treatment. Ann Intern Med 1991; 114: 472-481
39. American Academy of Pediatrics Committee on Infectious Diseases: treatment of Lyme borreliosis. Pediatrics 1991; 88: 176-179 [Erratum, Pediatrics 1991;88:840.]
40. Garon CF, Dorward DW, Corwin MD Structural features of Borrelia burgdorferi -- the Lyme disease spirochete: silver staining for nucleic acids. Scanning Microsc Suppl 1989; 3: 109-115
41. Whitmire WM, Garon CF Specific and nonspecific responses of murine B cells to membrane blebs of Borrelia burgdorferi. Infect Immun 1993; 61: 1460-1467
42. Bergstrom S, Garon CF, Barbour AG, MacDougall J Extrachromosomal elements of spirochetes. Res Microbiol 1992; 143: 623-628
43. Aurelius E, Johansson B, Skoldenberg B, Staland A, Forsgren M Rapid diagnosis of herpes simplex encephalitis by nested polymerase chain reaction assay of cerebrospinal fluid. Lancet 1991; 337: 189-192
44. Rotbart HA, Levin MJ, Murphy NL, Abzug MJ RNA target loss during solid phase hybridization of body fluids -- a quantitative study. Mol Cell Probes 1987; 1: 347-358