A multifunctional vector family for gene expression in mammalian cells

Gene

Volumn 149, Issue 2, 1994, Pages 387-388

  Dirks, Wilhelm ^a   Schaper, Fred ^b   Kirchhoff, Sabine ^b   Morelle, Christine ^c   Hauser, Hansjörg ^b  

^a Department of Human and Animal Cell Culture DSM German Collection of Microorganisms and Cell Cultures D 38124 Braunschweig Germany Tel ^*

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c SOLVAY S A   (Belgium)

Author keywords

Dicistronic expression; in vitro transcription; mammalian expression vector; MPSV LTR; poliovirus; recombinant DNA; T3 promoter; T7 promoter

Indexed keywords

ANIMAL CELL; ARTICLE; CLONING VECTOR; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC TRANSCRIPTION; MAMMAL CELL; NONHUMAN; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; PROMOTER REGION;

ANIMAL; CELL LINE; CLONING, MOLECULAR; GENE EXPRESSION; GENES, STRUCTURAL; GENETIC VECTORS; MAMMALS; OPEN READING FRAMES; PROMOTER REGIONS (GENETICS); TRANSCRIPTION, GENETIC; TRANSFECTION; TRANSLATION, GENETIC; XENOPUS LAEVIS;

ANIMALIA; MAMMALIA; POLIOVIRUS;

EID: 0028043504     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(94)90186-4     Document Type: Article

References (7)

1. Expression of human bone morphogenetic proteins-2 or -4 in murine mesenchymal progenitor C3H10T1/2 cells induces differentiation into distinct mesenchymal cell lineages
2. Vectors for efficient expression in mammalian fibroblastoid, myeloid and lymphoid cells via transfection or infection
3. Dicistronic transcription units for gene expression in mammalian cells
4. A new hybrid promoter directs transcription at identical start points in mammalian cells and in vitro
5. Both the 5′ untranslated region and the sequences surrounding the start site contribute to efficient translation in vitro
6. Tight control of gene expression in mammalian cells by tetraeycline-responsive promoters
7. Role of 3′ end sequences in infectivity of poliovirus transcripts made in vitro