Purification of the Escherichia coli integration host factor (IHF) in one chromatographie step

Gene

Volumn 147, Issue 1, 1994, Pages 149-150

  Filutowicz, Marcin ^a   Grimek, Herbert ^a   Appelt, Krzysztof ^b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b AGOURON PHARMACEUTICALS INC   (United States)

Author keywords

DNA binding bending protein; IHF; protein isolation

Indexed keywords

INTEGRATION HOST FACTOR;

ARTICLE; CHROMATOGRAPHY; DNA REPLICATION; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PURIFICATION; TECHNIQUE;

BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; DNA-BINDING PROTEINS; ESCHERICHIA COLI; INTEGRATION HOST FACTORS; SUPPORT, U.S. GOV'T, P.H.S.;

ESCHERICHIA COLI;

EID: 0028030486     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(94)90056-6     Document Type: Article

References (7)

1. Two alternative structures can be formed by IHF protein binding to the plasmid R6K γ origin
2. The integration host factor of Escherchia coli binds to multiple sites at plasmid R6K 7 origin and is essential for replication
3. A compact nucleoprotein structure is produced by binding of Escherichia coli integration host factor (IHF) to the replication origin of plasmid R6K
4. Integration host factor: a protein for all reasons
5. R promoter and its mutants
6. Overproduction of Escherichia coli integration host factor. a protein with nonidentical subunits
7. Bending and supercoiling of DNA at the attachment site of bacteriophage λ Trends