Regulation of pyrBI operon expression in Escherichia coli by UTP-sensitive reiterative RNA synthesis during transcriptional initiation

Genes and Development

Volumn 8, Issue 23, 1994, Pages 2904-2912

  Liu, Chongguang ^a   Heath, Lucie S ^a   Turnbough Jr , Charles L ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

promoter clearance; pseudotemplated transcription; Pyrimidine gene expression; RNA polymerase stuttering; transcriptional slippage

Indexed keywords

ARTICLE; BACTERIAL GENETICS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE MUTATION; NONHUMAN; OPERON; PRIORITY JOURNAL; RNA SYNTHESIS; TRANSCRIPTION INITIATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI; ESCHERICHIA COLI K12;

EID: 0028027955     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.8.23.2904     Document Type: Article

References (23)

1. Alper, M.D. and B.N. Ames. 1978. Transport of antibiotics and metabolite analogs by systems under cyclic AMP control: Positive selection of Salmonella typhimurium cya and crp mutants. J. Bacteriol. 133: 149-157.
2. Andersen, J.T., K.F. Jensen, and P. Poulsen. 1991. Role of transcription pausing in the control of the pyrE attenuator in Escherichia coli. Mol. Microbiol. 5: 327-333.
3. Chamberlin, M. and P. Berg. 1964. Mechanism of RNA polymerase action: Characterization of the DNA-dependent synthesis of polyadenylic acid. J. Mol. Biol. 8: 708-726.
4. Chan, C.L. and R. Landick. 1994. New perspectives on RNA chain elongation and termination by E. coli RNA polymerase. In Transcription: Mechanisms and regulation (ed. R. Conaway and J. Conaway), pp. 297-320. Raven Press, New York.
5. 2 of the pyrBI operon of Escherichia coli K12. J. Biol. Chem. 265: 19091-19099.
6. _. 1994. Nucleotide-specific transcriptional pausing in the pyrBI leader region of Escherichia coli K-12. J. Biol. Chem. 269: 18185-18191.
7. Dorit, R.L. and O. Ohara. 1991. Direct DNA sequencing of PCR products. In Current protocols in molecular biology (ed. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl), pp. 15.2.3. John Wiley & Sons, New York.
8. Jacques, J.-P. and D. Kolakofsky. 1991. Pseudo-templated transcription in prokaryotic and eukaryotic organisms. Genes & Dev. 5: 707-713.
9. Jacques, J.-P. and M.M. Susskind. 1990. Pseudo-templated transcription by Escherichia coli RNA polymerase at a mutant promoter. Genes &. Dev. 4: 1801-1810.
10. Jin, D.J. and C.L. Turnbough Jr. 1994. An Escherichia coli RNA polymerase defective in transcription due to its overproduction of abortive initiation products. J. Mol. Biol. 236: 72-80.
11. Kunkel, T.A., J.D. Roberts, and R.A. Zakour. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154: 367-382.
12. Landick, R. and C.L. Turnbough Jr. 1992. Transcriptional attenuation. In Transcriptional regulation (ed. S.L. McKnight and K.R. Yamamoto), pp. 407-446. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
13. Liu, C. and C.L. Turnbough Jr. 1989. Multiple control mechanisms for pyrimidine-mediated regulation of pyrBI operon expression in Escherichia coli K-12. J. Bacteriol. 171: 3337-3342.
14. Liu, J. and C.L. Turnbough Jr. 1994. Effects of transcriptional start site sequence and position on nucleotide-sensitive selection of alternative start sites at the pyrC promoter in Escherichia coli. J. Bacteriol. 176: 2938-2945.
15. 2 is the physiologically significant promoter for the pyrBI operon of Escherichia coli K-12. J. Bacteriol. 175: 2363-2369.
16. Martin, F.H. and I. Tinoco Jr. 1980. DNA-RNA hybrid duplexes containing oligo(dA:rU) sequences are exceptionally unstable and may facilitate termination of transcription. Nucleic Acids Res. 8: 2295-2299.
17. Neuhard, J. and P. Nygaard. 1987. Purines and pyrimidines. In Escherichia coli and Salmonella typhimurium: Cellular and molecular biology (ed. F.C. Neidhardt, J.L. Ingraham, K.B. Low, B. Magasanik, M. Schaechter, and H.E. Umbarger), pp. 445-473. American Society for Microbiology, Washington, D.C.
18. Roland, K.L., F.E. Powell, and C.L. Turnbough Jr. 1985. Role of translation and attenuation in the control of pyrBI operon expression in Escherichia coli K-12. J. Bacteriol. 163:991-999.
19. Roland, K.L., C. Liu, and C.L. Turnbough Jr. 1988. Role of the ribosome in suppressing transcriptional termination at the pyrBI attenuator of Escherichia coli K-12. Proc. Natl. Acad. Sci. 85: 7149-7153.
20. Turnbough, C.L. Jr. 1983. Regulation of Escherichia coli aspartate transcarbamylase synthesis by guanosine tetraphosphate and pyrimidine ribonucleoside triphosphates. J. Bacteriol. 153: 998-1007.
21. Turnbough, C.L. Jr., K.L. Hicks, and J.P. Donahue. 1983. Attenuation control of pyrBI operon expression in Escherichia coli K-12. Proc. Natl. Acad. Sci. 80: 368-372.
22. Xiong, X.F. and W.S. Reznikoff. 1993. Transcriptional slippage during the transcription initiation process at a mutant lac promoter in vivo. J. Mol. Biol. 231: 569-580.
23. Yanofsky, C., R.L. Kelley, and V. Horn. 1984. Repression is relieved before attenuation in the trp operon of Escherichia coli as tryptophan starvation becomes increasingly severe. J. Bacteriol. 158: 1018-1024.