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Volumn 35, Issue 2, 1994, Pages 215-218

Remarkable enhancement of binding affinity of Heterocycle-modified DNA to DNA and RNA. Synthesis, characterization and biophysical evaluation of N2-imidazolylpropylguanine and N2-imidazolylpropyl-2-aminoadenine modified oligonucleotides

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE;

EID: 0028014492     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(00)76514-2     Document Type: Article
Times cited : (40)

References (26)
  • 6
    • 84912970836 scopus 로고    scopus 로고
    • 6
  • 10
    • 84912982270 scopus 로고    scopus 로고
    • Modifications in the heterocycle portion of oligonucleotides may not affect the heteroduplex helical geometry of sugar that is necessary for RNase H cleavage.
  • 19
    • 84912974888 scopus 로고    scopus 로고
    • Standard protocol using an ABI 380B DNA synthesizer was modified by increasing the wait step after the pulse delivery of tetrazole to 900 sec. For deprotection conditions of the NPE-group see reference 10.
  • 20
    • 84913000343 scopus 로고    scopus 로고
    • Oligonucleotides were digested with a mixture of spleen phosphodiesterase, snake venom phosphodiesterase, and bacterial alkaline phosphatase to provide individual nucleosides which were analyzed by HPLC.
  • 24
    • 84913011873 scopus 로고    scopus 로고
    • Molecular minimization and dynamics studies were conducted using the Amber forcefield with the Insight and Discover programs (Biosym Inc., San Diego, CA). Four thousand steps of conjugate gradient minimization were used, followed by 1000 cycles of equilibration at 300°C, and 4000 steps of dynamics at 10 psec intervals.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.