Cysteine 148 in the Lactose Permease of Escherichia coli Is a Component of a Substrate Binding Site. 2. Site-Directed Fluorescence Studies

Biochemistry

Volumn 33, Issue 40, 1994, Pages 12166-12171

  Jianhua, Wu ^a   Kaback, H Ronald ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CYSTEINE; LACTOSE PERMEASE;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME BINDING; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; STRUCTURE ACTIVITY RELATION;

ANILINO NAPHTHALENESULFONATES; BASE SEQUENCE; BINDING SITES; CYSTEINE; ESCHERICHIA COLI; GALACTOSE; LACTOSE; LIGANDS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN STRUCTURE, SECONDARY; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP; SULFHYDRYL REAGENTS; TRISACCHARIDES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0028007043     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi00206a020     Document Type: Article

References (38)

1. Beyreuther, K., Bieseler, B., Ehring, R., & Müller-Hill, B. (1981) in Methods in Protein Sequence Analysis (Elzina, M., Ed) pp 139-148, Humana, Clifton, New York.
2. Bigelow, D. J., & Inesi, G. (1991) Biochemistry 30, 2113
3. Bradford, M. M. (1976) Anal. Biochem. 72, 248.
4. Brooker, R. J., & Wilson, T. H. (1986) J. Biol. Chem. 261, 11765
5. Consler, T. G., Persson, B. L., Jung, H., Zen, K. H., Jung, K., Privé, G. G. Verner, G. E., & Kaback, H. R. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 6934
6. Eftink, M. R. (1991) in Topics in Fluorescence Spectroscopy (Lakowicz, J. R., Ed.) Vol. 2, pp 52-126, Plenum Press, New York.
7. Fox, C. F., & Kennedy, E. P. (1965) Proc. Natl. Acad. Sci. U.S.A. 51, 891
8. Gupte, S. S., & Lane, L. K. (1979) J. Biol. Chem. 254, 10362
9. Gupte, S. S., & Lane, L. K. (1983) J. Biol. Chem. 258, 5005
10. Haugland, R. P. (1989) Handbook of Fluorescent Probes and Research Chemicals, p 25, Molecular Probes Inc., Eugene, OR
11. Hiratsuka, T. (1992) J. Biol. Chem. 267, 14941
12. Jung, H., Jung, K., & Kaback, H. R. (1994) Biochemistry (preceding paper in this issue).
13. Jung, K., Jung, H., Wu, J., Privé, G. G., & Kaback, H. R. (1993) Biochemistry 32, 12273.
14. Jung, K., Jung, H., & Kaback, H. R. (1994a) Biochemistry 33, 3980.
15. Jung, H., Jung, K., & Kaback, H. R. (1994b) Protein Sci. 3, 1052
16. Kaback, H. R. (1992) International Review of Cytology 137A (Jeon, K. W., & Friedlander, M., Eds.) pp 97-125, Academic Press, Inc.
17. Kennedy, E. P., Rumley, M. K., & Armstrong, J. B. (1974) J. Biol. Chem. 249, 33
18. Ksenzenko, M. Y., Kessel, D. H., & Rosen, B. P. (1993) Biochemistry 32, 13362
19. Laemmli, U. K. (1970) Nature (London) 227, 680
20. Lakowicz, J. R. (1983) Principles of Fluorescence Spectroscopy, pp 51-91, Plenum Press, New York.
21. Lolkema, J. S., & Walz, D. (1990) Biochemistry 29, 11180.
22. Lolkema, J. S., Carrasco, N., & Kaback, H. R. (1991) Biochemistry 30, 1284.
23. May, J M., & Beechem, J. M. (1993) Biochemistry 32, 2907
24. Menick, D. R., Sarkar, H. K., Poonian, M. S., & Kaback, H. R. (1985) Biochem. Biophys. Res. Commun. 132, 162
25. Menick, D. R., Carrasco, N., Antes, L., Patel, L., & Kaback, H. R. (1987) Biochemistry 26, 6638.
26. Neuhaus, J.-M., Soppa, J., Wright, J. K., Riede, I., Bocklage, H., Frank, R., & Overath, P. (1985) FEBS Lett. 185, 83
27. Phillips, W. J., & Cerione, R. A. (1991) J. Biol. Chem. 266, 11017.
28. Privé, G. G., Verner, G. E., Weitzman, C., Zen, K. H., Eisenberg, D., & Kaback, H. R. (1994) Acta Crystallogr. (in press)
29. Sahin-Tóth, M., & Kaback, H. R. (1993) Protein Sci. 2, 1024.
30. Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Plainview, NY.
31. Sanger, F., Nicklen, S., & Coulsen, A. R. (1977) Proc. Natl. Acad. Sci. U.S.A. 74, 5463
32. Sarkar, H. K., Viitanen, P. V., Poonian, M. S., & Kaback, H. R. (1986) Biochemistry 25, 2778
33. Teather, R. M., Bramhall, J., Riede, I., Wright, J. K., Fürst, M., Aichele, G., Wilhelm, V., & Overath, P. (1980) Eur. J. Biochem. 108, 223
34. Trumble, W. R., Viitanen, P. V., Sarkar, H. K., Poonian, M. S., & Kaback, H. R. (1984) Biochem. Biophys. Res. Commun. 119, 860.
35. van Iwaarden, P. R., Pastore, J. C., Konings, W. N., & Kaback, H. R. (1991) Biochemistry 30, 9595.
36. van Iwaarden, P. R., Driessen, A. J. M., Lolkema, J. S., Kaback, H. R., & Konings, W. N. (1993) Biochemistry 32, 5419
37. Viitanen, P. V., Menick, D. R., Sarkar, H. K., Trumble, W. R., & Kaback, H. R. (1985) Biochemistry 24, 7628.
38. Viitanen, P. V., Newman, M. J., Foster, D. L., Wilson, T. H., & Kaback H. R. (1986) Methods Enzymol. 125, 429