Bacterial aspects associated with the expression of a single-chain antibody fragment in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 42, Issue 4, 1994, Pages 595-603

  Somerville Jr , J E ^a   Goshorn, S C ^b   Fell, H P ^a   Darveau, R P ^a  

^a BRISTOL MYERS SQUIBB   (United States)

^b Poniard Pharmaceuticals Inc   (United States)

Author keywords

cell lysis; Protein folding; sFv; Single chain antibody

Indexed keywords

ANTIBODY FRAGMENT; UNCLASSIFIED DRUG;

ARTICLE; CYTOLYSIS; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PROTEIN FOLDING;

AMINO ACID SEQUENCE; ANIMAL; BIOTECHNOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; IMMUNOGLOBULIN FRAGMENTS; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; PROTEIN FOLDING; SUBCELLULAR FRACTIONS;

EID: 0027973672     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/BF00173926     Document Type: Article

References (37)

1. Bacterial expression and secretion of various single-chain Fv genes encoding proteins specific for a Salmonella serotypeB O-antigen
2. Definition of a common leukocyte cell-surface antigen (Lp95–150) associated with diverse cell-mediated immune functions
3. Escherichia coli secretion of an active chimeric antibody fragment
4. PrlA is important for the translocation of exported proteins across the cytoplasmic membrane of Escherichia coli
5. Single-chain antigen-binding proteins
6. Modulation of chemical composition and other parameters of the cell by growth rate
7. Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and Θ-80 transducing phages
8. XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection
9. High level Escherichia coli expression and production of a bivalent humanized antibody fragment
10. Outer membrane protein composition of Yersinia pestis at different growth stages and incubation temperatures
11. Expression of antibody fragments in Escherichia coli
12. Chimeric L6 anti-tumor antibody: genomic construction, expression and characterization of the antigen binding site
13. BR96 sFv-PE40, a potent single-chain immunotoxin that selectively kills carcinoma cells
14. Release of periplasmic enzymes and other physiological effects of β-lactamase overproduction in Escherichia coli
15. A comparison of strategies to stabilize immunoglobulin Fv-fragments
16. Genetic construction, expression and characterization of a single chain anti-carcinoma antibody fused to β-lactamase
17. Outer membranes of gram-negative bacteria XIX. Isolation from Pseudomonas aeruginosa PA01 and use in reconstitution and definition of the permeability barrier
18. Epitope mapping and use of anti-idiotypic antibodies to the L6 monoclonal anticarcinoma antibody
19. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli
20. The effect of folding catalysts on the in vivo folding process of different antibody fragments expressed in Escherichia coli
21. Growth measurement
22. Determinants of extracellular protein secretion in gram-negative bacteria
23. β-Lactamase I from Bacillus cereus
24. Secretion of β-lactamase into the periplasm of Escherichia coli: evidence for a distinct release step associated with a conformational change
25. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts
26. pH-dependence of the triose phosphate isomerase reaction
27. Mono- and bivalent antibody fragments produced in Escherichia coli: Engineering, folding and antigen binding
28. Synthesis of an Escherichia coli protein carrying a signal peptide mutation causes depolarization of the cytoplasmic membrane potential
29. A mutation in the amino terminus of a hybrid TrpC-TonB protein relieves overproduction lethality and results in cytoplasmic accumulation
30. Assembly of a functional immunoglobulin Fv fragment in Escherichia coli
31. folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulphides and cisSecretion and in vivo-prolines
32. The functional expression of antibody Fv fragments in Escherichia coli: improved vectors and a generally applicable purification technique
33. Extracellular production of cloned alpha-amylase by Escherichia coli
34. The ompA signal peptide directed secretion of staphylococcal nuclease A by Escherichia coli
35. An active single-chain antibody containing a cellulase linker domain is secreted by Escherichia coli
36. Effects of plasmid copy number and runaway plasmid replication on overproduction and excretion of β-lactamase from Escherichia coli