Cloning and characterization of a gene for the stage-specific 82-kDa surface antigen of metacyclic trypomastogotes of Trypanosoma cruzi

Molecular and Biochemical Parasitology

Volumn 65, Issue 1, 1994, Pages 161-169

  Araya, Jorge E ^a   Cano, Maria I ^a   Yoshida, Nobuko ^a   da Silveira, JoséFranco ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

82 kDa Glycoprotein; Gene cloning; gp85 sialidase superfamily; Metacyclic trypomastigote surface antigen; Multigene family; Trypanosoma cruzi

Indexed keywords

MEMBRANE ANTIGEN; PARASITE ANTIGEN;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENETIC ANALYSIS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; TRYPANOSOMA CRUZI; TRYPOMASTIGOTE;

AMINO ACID SEQUENCE; ANIMAL; ANTIGENS, PROTOZOAN; ANTIGENS, SURFACE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; DNA, PROTOZOAN; GENES, PROTOZOAN; GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MULTIGENE FAMILY; NEURAMINIDASE; RNA, MESSENGER; RNA, PROTOZOAN; SEQUENCE HOMOLOGY, AMINO ACID; SUPPORT, NON-U.S. GOV'T; TRYPANOSOMA CRUZI;

EID: 0027964678     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-6851(94)90124-4     Document Type: Article

References (35)

1. Partial inhibition of trypomastigotes entry into cultured mammalian cells by monoclonal antibodies against a surface glycoprotein of Trypanosoma cruzi
2. The interaction of a Trypanosoma cruzi surface protein with Vero cells and its relationship with parasite adhesion
3. A novel T. cruzi beparin binding protein promotes fibroblast adhesion and penetration of engineered bacteria and trypanosomes into mammalian cells
4. A novel cell surface trans-sialidase of Trypanosoma cruzi generates a stage-specific epitope required for invasion of mammalian cell
5. Evidence for the participation of the Ssp-3 antigen in the invasion of the nonphagocytic mammalian cells by Trypanosoma cruzi
6. T. cruzi metacyclic trypomastigotes: neutralization by the stage-specific monoclonal antibody 1G7 and immunogenicity of 90-kDa surface antigen
7. Resistance to acute Trypanosoma cruzi infection resulting from immunization of mice with 90-kDa antigen from metacyclic trypomastigotes
8. The stage-specific 90-kilodalton surface antigen of metacyclic trypomastigotes of T. cruzi
9. Involvement of the stage-specific 82-kilodalton adhesion molecule of Trypanosoma cruzi metacyclic trypomastigotes in host cell invasion.
10. The 35/50 surface antigen of Trypanosoma cruzi metacyclic trypomastigotes, an adhesion molecule involved in the host cell invasion
11. A superfamily of Trypanosoma cruzi surface antigens
12. The surface transialidase family of Trypanosoma cruzi
13. Trans-sialidase: a unique enzyme activity discovered in the protozoon Trypanosoma cruzi
14. Stage-specific surface antigens of metacyclic trypomastigotes of Trypanosoma cruzi identified by monoclonal antibody
15. Characterization of a DNA clone encoding the carboxy-terminal domain of a 90-kilodalton surface antigen of Trypanosoma cruzi metacyclic trypomastigotes
16. Single step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S-transferase
17. DNA sequencing with chain-termination inhibitors
18. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing
19. A comphrensive set sequence analysis programs for the VAX
20. Repetitive sequences in the ribosomal intergenic spacer of Trypanosoma cruzi
21. Plaque antibody selection: rapid immunological analysis of a large number of recombinant phages clones positive to sera raised against P. falciparum antigens
22. Rapid isolation of antigens from cells with staphylococcal protein A antibody absorbent: parameters of the interaction of antibody-antigen complex with protein
23. Cleavage of structural protein during the assembly of the head of bacteriophage T4
24. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications
25. Glycolipid anchoring of plasma membrane proteins
26. Proteins anchored via glycosylphosphatidylinositol and solubilizing phospholipases in Trypanosoma cruzi
27. Nucleotide sequence and transcription of a trypomastigote surface antigen of T. cruzi
28. An 85-kilodalton surface antigen gene family of Trypanosoma cruzi encodes polypeptides homologous to bacterial neuraminidases
29. The major 85-kDa surface antigen of the mammalian form of Trypanosoma cruzi is encoded by a large heterogeneous family of simultaneously expressed genes
30. Cytoplasmic regulation of mRNA function: the importance of the 3′ untranslated region
31. Post-transcriptional control of tubulin biosynthesis during leishmanial differentiation
32. Control of tubulin gene expression in the parasitic protozoan Leishmania enrietii
33. Trypanosoma brucei glycosomal glyceraldehyde-3-phosphate dehydrogenase genes are stage-regulated at transcriptional level
34. Transient activity of the Trypanosoma brucei variant surface glycoprotein gene promoter: Control of gene expression at the post-transcriptional level