Antigen–specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs

Nature Genetics

Volumn 7, Issue 1, 1994, Pages 13-21

  Green, L L ^a   Hardy, M C ^a   Maynard Currie, C E ^a   Tsuda, H ^a   Louie, D M ^a   Mendez, M J ^a   Abderrahim, H ^a   Noguchi, M ^a   Smith, D H ^a   Zeng, Y ^a   David, N E ^a   Sasai, H ^a   Garza, D ^a   Brenner, D G ^a   Hales, J F ^a   McGuinness, R P ^a   Capon, D J ^a   Klapholz, S ^a   Jakobovits, A ^a  

^a CELL GENESYS INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL FUSION; CHROMOSOME 14; CHROMOSOME MARKER; FIBROBLAST; FLUORESCENCE IN SITU HYBRIDIZATION; GENE LOCATION; GENE LOCUS; GENE MAPPING; GENOME; HUMAN; HUMAN CELL; HYBRID CELL; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RADIATION EXPOSURE; STATISTICAL ANALYSIS;

ADULT; AGE FACTORS; AMINO ACID SEQUENCE; ANIMAL; ANTIBODIES, MONOCLONAL; ANTIBODY FORMATION; BASE SEQUENCE; CHROMOSOMES, ARTIFICIAL, YEAST; GENES, IMMUNOGLOBULIN; HUMAN; HYBRIDOMAS; IMMUNOGLOBULINS, KAPPA-CHAIN; IMMUNOGLOBULINS, MU-CHAIN; MICE; MICE, TRANSGENIC; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SPECIES SPECIFICITY; SUPPORT, NON-U.S. GOV'T; TETANUS TOXIN; TETANUS TOXOID;

EID: 0027963484     PISSN: 10614036     EISSN: 15461718     Source Type: Journal    
DOI: 10.1038/ng0594-13     Document Type: Article

References (31)

1. Brüggemann, M. et al. A repertoire of monoclonal antibodies with human heavy chains from transgenic mice. Proc. natn. Acad. Sci. U.S.A. 86, 6709-6713 (1989).
2. Brüggemann, M. et al. Human antibody production in transgenic mice: expression from 100 kb of the human IgH locus. Eur. J. Immunol. 27, 1323-1326 (1991).
3. Taylor, L.D. et al. A transgenic mouse that expresses a diversity of human sequence heavy and light chain immunoglobulins. Nucl. Acids Res.20, 6287-6295 (1992).
4. Tuaillon, N., Taylor, L.D., Lonberg, N., Tucker, P.W. & Capra, J.D. Human immunoglobulin heavy-chain minilocus recombination in transgenic mice: Gene-segment use in and transcripts. Proc. natn. Acad. Sci. U.S.A. 90, 3720-3724 (1993).
5. Choi, T.K. et al. Transgenic mice containing a human heavy chain immunoglobulin gene fragment cloned in a yeast artificial chromosome.Nature Genet. 4, 117-123 (1993).
6. Davies, N.P. et al. Creation of mice expressing human antibody light chains by introduction of a yeast artificial chromosome containing the core region of the human immunoglobulin locus. Bio/Technology 11, 911-914 (1993).
7. Jakobovits, A. et al. Germ-line transmission and expression of a human-derived yeast artificial chromosome. Nature 362, 255-258 (1993).
8. Shin, E.K. et al. Physical map of the 3' region of the human immunoglobulin heavy chain locus: clustering of autoantibody-related variable segments in one haplotype. EMBO J. 10, 3641-3645 (1991).
9. Matsuda, F. et al. Structure and physical map of 64 variable segments in the 3' 0.8-megabase region of the human immunoglobulin heavy-chain locus. Nature Genetics 3, 88-94 (1993).
10. Weichhold, G.M., Ohnheiser, R. & Zachau, H.G. The human immunoglobulin locus consists of two copies that are organized in opposite polarity. Genomes 16, 503-511 (1993).
11. Reid, L., Gregg, R.G., Smithies, O. & Koller, B.H. Regulatory elements in the introns of the human HPRT gene are necessary for its expression in embryonic stem cells. Proc. natn. Acad. Sci. U.S.A. 87, 4299-4303 (1990).
12. Yamada, M. et al. Preferential utilization of specific immunoglobulin heavy chain diversity and joining segments in adult human peripheral blood B lymphocytes. J. exp. Med. 173, 395-407 (1991).
13. Marks, J.D., Tristem, M., Karpas, A. & Winter, G. Oligonucleotide primers for polymerase chain reaction amplification of human immunoglobulin variable genes and design of family-specific oligonucleotide probes. Eur. J. Immunol. 21, 985-991 (1991).
14. Kabat, E.A., Wu, T.T., Perry, H.M., Gotteman, K.S. & Foeller, C. Sequences of Proteins of Immunological Interest, Fifth Edition, U. S. Department of Health and Human Services, NIH publication No. 91, 3242 (1991).
15. Schroeder, H.W., Jr. & Wang, J.Y. Preferential utilization of conserved Immunoglobulin heavy chain variable gene segments during human fetal life. Proc. natn. Acad. Sci. U.S.A. 87, 6146-6150 (1990).
16. H6 immunoglobulin genes in early human B-cell ontogeny. Scand. J. Immunol. 30, 493-497 (1989).
17. Feeney, A.J. Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences. J. exp. Med. 172, 1377-1390 (1990).
18. Jakobovits, A. et al. Analysis of homozygous mutant chimeric mice: Deletion of the immunoglobulin heavy-chain joining region blocks B-cell development and antibody production. Proc. natn. Acad. Sci. U.S.A. 90, 2551-2555 (1993).
19. Abney, E.R., Cooper, M.D., Kearney, J.F., Lawton, A.R. & Parkhouse, R.M.E. Sequential expression of immunoglobulin on developing mouse B lymphocytes: a systematic survey that suggests a model for the generation of immunoglobin isotype diversity. J. Immunol. 120, 2041-2049 (1987).
20. Hardy, R.R., Condie, C.E., Shinton, S.A., Kemp, J.D. & Hayakawa, K. Resolution and characterization of pro-B and pre-pro-B cell stages in normal bone marrow. J. exp. Med. 173, 1213-1225.
21. H locus. EMBO J. 7, 727-738 (1988).
22. H) pseudogene that deletes the second complementarity-determining region. Proc. natn. Acad. Sci. U.S.A. 81, 5194-5198 (1984).
23. Lorenz, W., Straubinger, B. & Zachau, G.G. Physical map of the human immunoglobulin locus and its implications for the mechanisms of V.-J. rearrangement. Nucl. Acids Res. 15, 9667-9676 (1987).
24. Hieter, P.A., Maizel, V.J., Jr. & Leder, P. Evolution of human immunoglobulin J region genes. J. biol. Chem. 257, 1516-1522 (1982).
25. Lorenz, W., Schäble, K.F., Thiebe, R., Stavneser, J. & Zachau, H.G. The J. proximal region of the human locus contains three uncommon V. genes which are arranged in opposite transcriptional polarities. Molec. Immunol. 25, 479-484 (1988).
26. Klobeck, H.-G., Combriato, G. & Zachau, H.G. The V-J-intergenic region of the human locus. Biol. Chem. 370, 1007-1012 (1989).
27. Neumaier, P.S. & Zachau, H.G. Nucleotide sequence of a region downstream of the mouse C. immunoglobulin gene. Nucl. Acids Res. 11, 3631-3636 (1983).
28. Sambrook, J., Fritsch, E.F. & Maniatis, J. (eds) Molecular Cloning, A Laboratory Manual (Cold Spring Harbor Laboratory Press, New York, 1989).
29. Ichihara, Y., Matsuoka, H. & Kurosawa, Y. Organization of human immunoglobulin heavy chain diversity gene loci. EMBO J. 7, 4141-4150 (1988).
30. Flanagan, J.G. & Rabbitts, T.H. The sequence of a human immunoglobulin epsilon heavy chain constant gene, and evidence for three non-allelic genes. EMBO J. 1, 655-660 (1982).
31. Ravetch, J.V., Siebenlist, U., Korsmeyer, S., Waldmann, T. & Leder, P. Structure of the human immunogloblin locus: Characterization of embryonic and rearranged J and D genes. Cell 27, 583-591 (1981).