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Volumn 116, Issue 12, 1994, Pages 5467-5468

Value of General Acid-Base Catalysis to Ribonuclease A

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EID: 0027949817     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja00091a060     Document Type: Article
Times cited : (134)

References (36)
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    • For reviews, see: (a)
    • For reviews, see: (a) Richards, F. M.; Wyckoff, H. W. Enzymes 1971, 4, 647-806.
    • (1971) Enzymes , vol.4 , pp. 647-806
    • Richards, F.M.1    Wyckoff, H.W.2
  • 4
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    • Nucleic Acids and Interactive Proteins; Jurnak, F. A., McPherson, A., Eds.; Wiley: New York
    • Wlodawer, A. In Biological Macromolecules and Assemblies, Vol. II, Nucleic Acids and Interactive Proteins; Jurnak, F. A., McPherson, A., Eds.; Wiley: New York, 1985; pp 393-439.
    • (1985) Biological Macromolecules and Assemblies , vol.II , pp. 393-439
    • Wlodawer, A.1
  • 6
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    • Neuberger, A., Brocklehurst, K., Eds.; Elsevier: New York
    • Eftink, M. R.; Biltonen, R. L. In Hydrolytic Enzymes; Neuberger, A., Brocklehurst, K., Eds.; Elsevier: New York, 1987; pp 333-376.
    • (1987) Hydrolytic Enzymes , pp. 333-376
    • Eftink, M.R.1    Biltonen, R.L.2
  • 7
    • 0004155427 scopus 로고    scopus 로고
    • The enzyme also catalyzes (albeit inefficiently, see: in press) the hydrolysis of the 2',3'-cyclic phosphodiester product of RNA cleavage.
    • The enzyme also catalyzes (albeit inefficiently, see: Thompson, J. E.; Venegas, F. D.; Raines, R. T. Biochemistry, in press) the hydrolysis of the 2',3'-cyclic phosphodiester product of RNA cleavage.
    • Biochemistry
    • Thompson, J.E.1    Venegas, F.D.2    Raines, R.T.3
  • 22
    • 0023613953 scopus 로고
    • Mutations in the cDNA that codes for RNase A were made by oligonucleotide-mediated site-directed mutagenesis (). Mutant cDNAs were expressed in Escherichia coli under the control of the T7 RNA polymerase promoter, and the resulting proteins were purified by standard methods (ref 8).
    • Mutations in the cDNA that codes for RNase A were made by oligonucleotide-mediated site-directed mutagenesis (Kunkel, T. A.; Roberts, J. D.; Zakour, R. A. Methods Enzymol. 1987, 154, 367-382). Mutant cDNAs were expressed in Escherichia coli under the control of the T7 RNA polymerase promoter, and the resulting proteins were purified by standard methods (ref 8).
    • (1987) Methods Enzymol. , vol.154 , pp. 367-382
    • Kunkel, T.A.1    Roberts, J.D.2    Zakour, R.A.3
  • 26
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    • 2 cleavage are about 2-fold higher than those reported previously (). This disparity may result from a difference in the pH of the assay solutions (6.0 vs 7.5).
    • 2 cleavage are about 2-fold higher than those reported previously (Davis, A. M.; Regan, A. C.; Williams, A. Biochemistry 1988, 27, 9042-9047). This disparity may result from a difference in the pH of the assay solutions (6.0 vs 7.5).
    • (1988) Biochemistry , vol.27 , pp. 9042-9047
    • Davis, A.M.1    Regan, A.C.2    Williams, A.3
  • 27
    • 0024435026 scopus 로고    scopus 로고
    • 4-fold less activity for RNA cleavage than does wild-type human angiogenin (Shapiro, R.; Vallee, B. L. Biochemistry 1989, 28, 7401-7408).
    • 4-fold less activity for RNA cleavage than does wild-type human angiogenin (Shapiro, R.; Vallee, B. L. Biochemistry 1989, 28, 7401-7408).
    • Kim, J.-S.1    Raines, R.T.2
  • 30
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    • Gerlt and Gassman have proposed the existence of the same phosphorane intermediate as in the Breslow mechanism (). In the Gerlt and Gassman mechanism, however, H119 serves only to protonate the 5" oxygen, a role that is not in conflict with our results.
    • Gerlt and Gassman have proposed the existence of the same phosphorane intermediate as in the Breslow mechanism (Gerlt, J. A.; Gassman, P. G. Biochemistry 1993, 32, 11943-11952). In the Gerlt and Gassman mechanism, however, H119 serves only to protonate the 5" oxygen, a role that is not in conflict with our results.
    • (1993) Biochemistry , vol.32 , pp. 11943-11952
    • Gerlt, J.A.1    Gassman, P.G.2
  • 32
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    • and references therein.
    • Jencks, W. P. Chem. Rev. 1985, 85, 511-527 and references therein.
    • (1985) Chem. Rev. , vol.85 , pp. 511-527
    • Jencks, W.P.1


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