Human cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase: Expression, purification, and characterization of recombinant wild-type and Cys129 mutant enzymes

Archives of Biochemistry and Biophysics

Volumn 312, Issue 1, 1994, Pages 1-13

  Rokosz, Laura L ^a,b   Boulton, David A ^a   Butkiewicz, Elise A ^a   Sanyal, Gautam ^a   Cueto, Maria A ^a   Lachance, Paul A ^b   Hermes, Jeffrey D ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0027932251     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1006/abbi.1994.1273     Document Type: Article

References (47)

1. Goldstein, J. L., and Brown, M. S. (1984) J. Lipid Res. 25, 1450-1461.
2. Goldstein, J. L., and Brown, M. S. (1977) Annu. Rev. Biochem. 46, 897-930.
3. Brown, M. S., and Goldstein, J. L. (1980) J. Lipid Res. 21, 505-517.
4. Chang, T., and Limanek, J. S. (1980) J. Biol. Chem. 255, 7787-7795.
5. Gil, G., Goldstein, J. L., Slaughter, C. A., and Brown, M. S. (1986) J. Biol. Chem. 261, 3710-3716.
6. Mehrabian, M., Callaway, K. A., Clarke, C. F., Tanaka, R. D., Greenspan, M., Lusis, A. J., Sparkes, R. S., Mohandas, T., Edmond, J., Fogelman, A. M., and Edwards, P. A. (1986) J. Biol. Chem. 261, 16249-16255.
7. Clarke, F. C., Tanaka, R. D., Svenson, K., Wamsley, M., Fogelman, A. M., and Edwards, P. A. (1987) Mol. Cell. Biol. 7, 3138-3146.
8. Clinkenbeard, K. D., Reed, W. D., Mooney, R. A., and Lane, M. D. (1975) J. Biol. Chem. 250, 3108-3116.
9. Ayte, J., Gil-Gomez, G., and Hegardt, F. G. (1990) Nucleic Acids Res. 18, 3642.
10. Kittar-Cooley, P. A., Wang, H. L., Mende-Mueller, L. M., and Mi-ziorko, H. M. (1990) Arch. Biochem. Biophys. 283, 523-529.
11. Chao, Y., Chen, J. S., Hunt, V. M., Kuron, G. W., Karkas, J. D., Liou, R., and Alberts, A. W. (1991) Eur. J. Clin. Pharmacol. 40(Suppl. 1), 811-814.
12. Ditschuneit, H. H., Kuhn, K., and Ditschuneit, H. (1991) Eur. J. Clin. Pharmacol. 40(Suppl. 1), 827-832.
13. Reihner, E., Rudling, M., Stahlberg, D., Berglund, L., Ewerth, S., Bjorkhem, I., Einersson, K., and Angelin, B. (1990) N. Engl. J. Med. 323, 224-228.
14. Gil, G., Smith, J. R., Goldstein, J. L., and Brown, M. S. (1987) Proc. Natl. Acad. Sci. USA 84, 1863-1866.
15. Clinkenbeard, K. D., Sugiyama, T., Reed, W. D., and Lane, M. D. (1975) J. Biol. Chem. 250, 3124-3135.
16. Miziorko, H. M., and Behnke, C. E. (1985) J. Biol. Chem. 260, 13513-13516.
17. Miziorko, H. M., Clinkenbeard, K. D., Reed, W. D., and Lane, M. D. (1975) J. Biol. Chem. 250, 5768-5773.
18. Vollmer, S. H., Mende-Mueller, L. M., and Miziorko, H. M. (1988) Biochemistry 27, 4288-4292.
19. Johnson, B. A., McClain, S. G., Doran, E. R., Tice, G., and Kirsch, M. A. (1990) BioTechniques 8, 424-429.
20. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. USA 74, 5463-5467.
21. Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubbendorf, J. W. (1990) Methods Enzymol. 185, 60-89.
22. Manvalan, P., and Johnson, W. C., Jr. (1987) Anal. Biochem. 167, 76-85.
23. Lowe, D. M., and Tubbs, P. K. (1985) Biochem. J. 227, 591-599.
24. Middleton, B. (1972) Biochem. J. 126, 35-47.
25. Daniels, S. B., Cooney, E., Sofia, M. J., Chakravarty, P. K., and Katzenellenbogen, J. A. (1983) J. Biol. Chem. 258, 15046-15053.
26. Bradford, M. M. (1976) Anal. Biochem. 72, 143-147.
27. Laemmli, U. K. (1970) Nature 227, 680-685.
28. Towbin, H., Staehelin, T., and Gordon, J. (1979) Proc. Natl. Acad. Sei. USA 76, 4350-4354.
29. Russ, A. P., Ruzicka, V., Maerz, W., Appelhans, H., and Grob, W. (1992) Biochim. Biophys. Acta 1132, 329-331.
30. Bairoch, A., and Boeckmann, B. (1993) Nucleic Acids Res. 21, 3093-3096.
31. George, G. D., Barker, W. C., and Hunt, L. T. (1986) Nucleic Acids Res. 14, 11-16.
32. Burks, C., Cassidy, M., Cinkosky, M. J., Cumella, K. E., Gilna, P., Hayden, J. E-D., Keen, G. M., Kelley, T. A., Kelly, M., Kristofferson, D., and Ryals, J. (1991) Nucleic Acids Res. 19(Suppl. 1), 2221-2225.
33. Misra, I., Chakravarthy, N., and Miziorko, H. M. (1993) J. Biol. Chem. 268, 12129-12135.
34. Bordo, D., and Argos, P. (1991) J. Mol. Biol. 217, 721-729.
35. Lowe, D. M., and Tubbs, P. K. (1985) Biochem. J. 227, 601-607.
36. Mayer, R. J., Louis-Flamberg, P., Elliott, J. D., Fisher, M., and Leber, J. (1990) Biochem. Biophys. Res. Commun. 169, 610-616.
37. Main, A. R. (1973) in Essays in Toxicology (Hays, W. J., Ed.), Vol. 4, pp. 59-105, Academie Press, New York.
38. Greenspan, M. D., Yudkovitz, J. B., Lo, C. L., Chen, J. S., Alberts, A. W., Hunt, V. M., Chang, M. N., Yang, S. S., Thompson, K. L., Chiang, Y. P., Chabala, J. C., Monaghan, R. L., and Schwartz, R. L. (1987) Proc. Natl, Acad. Sci. USA 84, 7488-7492.
39. Stewart, P. R., and Rudney, H. (1966) J. Biol. Chem. 241, 1222-1228.
40. Ayte, J., Gil-Gomez, G., Haro, D., Marrero, P. F., and Hegardt, F. G. (1990) Proc. Natl. Acad. Sci. USA 87, 3874-3878.
41. Bruice, T., and Benkovic, S. (1966) Bioorganic Mechanisms, W. A. Benjamin, New York.
42. Jencks, W. (1969) Catalysis in Chemistry and Enzymology, McGraw-Hill, New York.
43. Thompson, S., Mayerl, F., Peoples, O. P., Masamune, S., Sinskey, A. J-, and Walsh, C. T. (1989) Biochemistry 28, 5735-5742.
44. Aldridge, D. C., Giles, D., and Turner, W. B. (1971) J. Chem. Soc. (C), 3888-3891.
45. Omura, S., Tomoda, H., Kumagai, H., Greenspan, M. D., Yudkovitz, J. B., Chen, J. S., Alberts, A. W., Martin, I., Mochales, S., Monaghan, R. L., Chabala, J. C., Schwartz, R. E., and Patchett, A. A. (1987) J. Antibiot. 40, 1356-1357.
46. Tomoda, H., Kumagai, H., Tanaka, H., and Omura, S. (1987) Biochim. Biophys. Acta 922, 351-356.
47. Greenspan, M. D., Bull, H. G., Yudkovitz, J. B., Hanf, D. P., and Alberts, A. W. (1993) Biochem. J. 289, 889-895.