NuSerum, a synthetic serum replacement, supports chondrogenesis of embryonic chick limb bud mesenchymal cells in micromass culture

In Vitro Cellular & Developmental Biology - Animal

Volumn 29, Issue 12, 1993, Pages 917-922

  Wong, Mayme ^a   Tuan, Rocky S ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

chondrogenesis; limb bud mesenchyme; micromass culture

Indexed keywords

DNA; GROWTH FACTOR; HORMONE; LEUCINE; PROTEIN; PROTEOGLYCAN; SULFATE; THYMIDINE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DENSITY; CELL DIFFERENTIATION; CELL GROWTH; CELL PROLIFERATION; CHICKEN; CHONDROGENESIS; CONTROLLED STUDY; DNA SYNTHESIS; EMBRYO; LIMB BUD; LIQUID SCINTILLATION COUNTING; MESENCHYME CELL; NONHUMAN; PROTEIN SYNTHESIS; SERUM;

ANIMAL; BLOOD PROTEINS; BLOOD SUBSTITUTES; CARTILAGE; CELL DIFFERENTIATION; CELLS, CULTURED; CHICK EMBRYO; CULTURE MEDIA, SERUM-FREE; DNA; EXTREMITIES; GLYCOSAMINOGLYCANS; MESODERM; PROTEINS; PROTEOGLYCANS; SERUM ALBUMIN, BOVINE;

EID: 0027880210     PISSN: 10712690     EISSN: 1543706X     Source Type: Journal    
DOI: 10.1007/BF02634229     Document Type: Article

References (24)

1. Stage-related capacity for limb chondrogenesis in cell culture
2. Methods for growth of cultured cells in serum-free medium
3. Embryonic chick cartilage produces its own somatomedin-like peptide to stimulate cartilage growth in vitro
4. Clonal stability and phenotypic expression of chick cartilage cells in vitro
5. Characterization of bone-derived chondrogenesis-stimulating activity on embryonic limb mesenchymal cells in vitro
6. Limb bud chondrogenesis in cell culture, with particular reference to serum concentration in the culture medium
7. Morphological and biochemical differentiation of limb bud cells cultured in chemically defined medium
8. A serum-free medium supplemented with multiplication-stimulating activity (MSA) supports both proliferation and differentiation of chondrocytes in primary culture
9. Fibronectin gene expression during limb cartilage differentiation
10. Specific staining of sulfate groups with alcian blue at low pH
11. Regulation by growth hormone of number of chondrocytes containing IGF-I in rat growth plate
12. Osteogenesis in cultures of limb mesenchymal cells
13. Microtiter micromass cultures of limb-bud mesenchymal cells
14. A serum substitute promotes osteoblast-like phenotypic expression in cultured cells from chick calvariae
15. Polyionic regulation of cartilage development: promotion of chondrogenesis in vitro by polylysine is associated with altered glycosaminoglycan biosynthesis and distribution
16. Regulation of chondrogenesis by heparan sulfate and structurally related glycosaminoglycans
17. Chondrogenesis of limb bud mesenchyme in vitro: stimulation by cations
18. Cell and matrix interactions during limb chondrogenesis in vitro
19. Insulin-like growth factor I and II and insulin-like growth factor binding protein-2 RNAs are expressed in adjacent tissues within rat embryonic and fetal limbs
20. In vitro effects of glucose and growth factors in limb bud and mandibular arch chondrocytes maintained at various serum concentrations
21. Inhibition of limb chondrogenesis by fibronectin
22. Differential survival of cartilage and muscle cells in chick limb-bud cell cultures maintained in chemically defined and serum-containing media
23. Ionic regulation of chondrogenesis
24. The effect of cell population density on the developmental fate of reaggregating mouse limb bud mesenchyme