Nonradioactive In Situ Hybridization Methods for Drosophila Embryos Detecting Signals by Immunogold‐Silver or Immunoperoxidase Method for Electron Microscopy: (electron microscopy/pre‐embedding/transcripts/Drosophila embryo)

Development, Growth & Differentiation

Volumn 35, Issue 6, 1993, Pages 617-623

  Amikura, Reiko ^a   Kobayashi, Satoru ^a   Endo, Keita ^a   Okada, Masukichi ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL EXPERIMENT; ARTICLE; DROSOPHILA; ELECTRON MICROSCOPY; EMBRYO; EMBRYO DEVELOPMENT; EMBRYO SEGMENTATION; GENETIC TRANSCRIPTION; IMMUNOGOLD STAINING; IMMUNOPEROXIDASE STAINING; IN SITU HYBRIDIZATION; NONHUMAN; TECHNIQUE;

EID: 0027770686     PISSN: 00121592     EISSN: 1440169X     Source Type: Journal    
DOI: 10.1111/j.1440-169X.1993.00617.x     Document Type: Article

References (24)

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2. Localization of gold in biological tissue. A photochemical method of light and electron microscopy
3. Apical localization of pair‐rule transcripts requires 3′ sequences and limits protein diffusion in the Drosophila blastoderm embryo
4. oskar organizes the germ plasm and directs localization of the posterior determinant nanos
5. tudor, a posterior‐group gene of Drosophila melanogaster, encodes a novel protein and an mRNA localized during mid‐oogenesis
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7. Spatial distribution of transcripts from the segmentation gene fushi tarazu during Drosophila embryonic development
8. A protein component of Drosophila polar granule is encoded by vasa and has extensive sequence similarity to ATP‐dependent helicases
9. Immunogold‐silver staining: New method of immunostaing with enhanced sensitivity
10. The correct activation of Antenapedia and bithorax complex genes requires fushi tarazugene
11. oskar mRNA is localized to the posterior pole of the Drosophila oocyte
12. Presence of mitochondrial IrRNA outside mitochondria in germ plasm of Drosophila melanogaster
13. Cloning and transcriptional analysis of the segmentation gene fushi tarazu of Drosophila
14. The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor‐4A
15. Borders of parasegments in Drosophila embryos are delimited by the fushi tarazuand even‐skipped genes
16. Distribution of myosin heavy chain mRNA in embryonic muscle tissue visualized by ultrastructural in situhybridization
17. Nucleolar organization of HeLa cells as studied by in situ hybridization
18. Ultrastructural visualization of cytoskeletal mRNA and their associated proteins using double‐label in situ hybridization
19. A low‐viscosity epoxy resin embedding medium for electron microscopy
20. Presence of rRNA in the heavy bodies of sea urchin eggs
21. A non‐radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback
22. Characterization of AL Amyloid protein identified by immunoelectron microscopy: A simple method using the protein A‐gold technique
23. Nanos is the localized posterior determinant in Drosophila
24. o mRNA in myelin‐forming schwann cells