Cloning and characterization of the gene encoding ATP-dependent phospho-enol-pyruvate carboxykinase in Trypanosoma cruzi: comparison of primary and predicted secondary structure with host GTP-dependent enzyme

Gene

Volumn 136, Issue 1-2, 1993, Pages 69-77

  Linss, Jutta ^a   Goldenberg, Samuel ^a   Urbina, Julio A ^b   Amzel, L Mario ^c  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b lnstituto Venezolano de Investigations Cientificas Centra de Bioquímica y Biofisica Laboratorio de Química Biologica ^*  (Venezuela)

^c JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

consensus sequences; Nucleotide sequence; protein functional domains; sequence homology

Indexed keywords

CARBOXYKINASE; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ENZYME STRUCTURE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; TRYPANOSOMA CRUZI;

ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; ANIMAL; BASE SEQUENCE; CLONING, MOLECULAR; COMPARATIVE STUDY; DNA, PROTOZOAN; GENES, PROTOZOAN; GUANOSINE TRIPHOSPHATE; HUMAN; MOLECULAR SEQUENCE DATA; PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP); PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); PROTEIN STRUCTURE, SECONDARY; SEQUENCE HOMOLOGY, AMINO ACID; SUPPORT, NON-U.S. GOV'T; TRYPANOSOMA CRUZI;

EID: 0027725284     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(93)90449-D     Document Type: Article

References (51)

1. Rat hepatic cytosolic phosphoenolpyruvate carboxykinase (GTP): Structures of the protein, messenger RNA and gene
2. Discontinuous transcription and antigenic variation in trypanosomes
3. Aerobic fermentation of glucose by trypanosomatids
4. Protein and aminoacid catabolism in Trypanosoma cruzi
5. End products and enzyme levels of aerobic glucose fermentation in Trypanosomatids
6. Nova tripanosomiase humana Estudos sobre a mtorfolojia e o cyclo de cultivo do Schyzotrypanum cruzi n gen n sp agente etiolojico de nova morbidade do homen
7. Empirical prediction of protein conformation
8. Phospho-enol-pyruvate carboxykinase (guanosine 5'-triphosphate) from rat liver cytosol. Dual cation requirement for the carboxylation reaction
9. Phospho-enol- pyruvate carboxykinase (guanosine 5'-triphosphate) from rat liver cytosol. Divalent cation requirement in the decarboxylation reaction
10. Nucleotide sequence of the mRNA encoding the cytosolic form of phosphoeno/pyruvate carboxykinase (GTP) from the chicken
11. GTP-binding domain: three consensus sequence elemts with distinct spacing
12. A comprehensive set of sequence analysis programs for the VAX
13. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins
14. A family of closely related ATP-binding sub-units from prokariotic and eukaryotic cells
15. Cloning of a cDNA encoding phosphoenolpyruvate carboxykinase from Haemonchus contortus
16. A glycosomal protein (p60) which is predominantly expressed in procyclic Trypanosoma brucei
17. A simple method for displaying the hydropathic character of a protein
18. Structure and expression of two Trypanosoma cruzi genes encoding antigenic proteins bearing repetitive epitopes
19. RNA molecular weight determination by gel electrophoresis under denaturating conditions: a critical reexamination
20. Cysteine 288: an essential hyperreactive thiol of cytosolic phosphoenol-pyruvate carboxykinase (GTP)
21. A reactive cysteine in avian liver phos-phoenolpyruvate carboxykinase
22. Characterization of the spliced-leader genes and transcripts in Trypanosoma cruzi
23. Metabolic control of gene expression: In vivo studies with transgenic mice
24. NMR studies of the interactions of substrates with enzymes and their peptide fragments
25. The structure of cat muscle pyruvate kinase
26. Crystallographic studies on the structure and catalytic activity of pyruvate kinase from skeletal muscle
27. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis
28. Site-directed mutagenesis and DNA sequence of pckA of Rhizobium NGR234, encoding phospho-enolpyruvate carboxykinase: gluconeogenesis and host-dependent symbiotic phenotype
29. Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 Å resolution: implications for the mechanism of GTP hydrolysis
30. Trypanosome glycosomal protein p60 is homologous to phosphoenolpyruvate carboxykinase
31. Structurally and catalytically important residues in the phosphate binding loop of adenylate kinase of Escherichia coli
32. Sequence of the phosphoenolpyruvate carboxykinase-encoding cDNA from the rumen anaerobic fungus Neocallimastix frontalis: comparison of the amino acid sequence with animals and yeast
33. DNA sequencing with chain terminating inhibitors
34. Structural and functional relationships in the adenylate kinase family
35. The nucleotide sequence of the aroF gene of Escherichia coli and the amino acid sequence of the encoded protein, the tyrosine-sensitive 3-deoxy-d-arabino-heptulosonate synthase
36. Detection of specific sequences among DNA fragments separated by gel electrophoresis
37. A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate
38. Three-dimensional structure of the complex of guanylate kinase from yeast and its substrate GMP
39. Nucleotide sequence of the phosphoenolpyruvate carboxykinase gene from Saccharomyces cerevisiae
40. The phospho-enol-pyruvate carboxykinase of Trypanosoma (Schizotrypanum) cruzi epimastigotes: molecular, kinetic and regulatory properties
41. + dependent glutamate dehydrogenase
42. Inhibition of phospho-eno/-pyruvate carboxykinase from Trypanosoma (Schizotrypanum) cruzi epimastigotes by 3-mercaptopicolinic acid: in vitro and in vivo studies
43. 2 fixation on phospho-enol-pyruvate
44. 1H NMR studies of the nucleotide sites in solution
45. Distantly related sequences in the a and α-subunits of ATP-synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide-binding fold
46. The unique role of kidney in gluconeogenesis in the chicken. The significance of a cytosolic form of phosphoenolpyruavte carboxykinase
47. Mitochondrial phosphoe-nolpyruavte carboxykinase from the chicken. Comparison of the cDNA and protein sequences with the cytosolic enzyme
48. Efficient isolation of genes by using antibody probes