Display of biologically active proteins on the surface of filamentous phages: a cDNA cloning system for selection of functional gene products linked to the genetic information responsible for their production

Gene

Volumn 137, Issue 1, 1993, Pages 69-75

  Crameri, Reto ^a   Suter, Mark ^a  

^a SWISS INSTITUTE OF ALLERGY AND ASTHMA RESEARCH SIAF   (Switzerland)

Author keywords

clonal selection; dimeric proteins; Fos; Gene enrichment; Jun; prokaryotic expression

Indexed keywords

COMPLEMENTARY DNA; GENE PRODUCT; PROTEIN;

BACTERIOPHAGE; CELL SURFACE; CLONING; CONFERENCE PAPER; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE LIBRARY; GENES, FOS; GENES, JUN; INOVIRUS; LEUCINE ZIPPERS; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SUPPORT, NON-U.S. GOV'T;

PROKARYOTA;

EID: 0027723182     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(93)90253-Y     Document Type: Article

References (52)

1. Expression and purification of the leucine zipper and DNA-binding domains of Fos and Jun: both Fos and Jun contact DNA directly
2. Recombinant DNA
3. Aspergillus fumigatus allergen I, a major IgE-binding protein, is a member of the mitogillin family of cytotoxins
4. Aspergillus fumigatus: identification of 16, 18 and 45 kDa antigens recognized by human IgG and IgE antibodies and murine monoclonal antibodies
5. Combinatorial immunoglobulin libraries on the surface of phage (Phabs): rapid selection of antigenspecific Fabs
6. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site
7. Hormone phage: an enrichment method for variant proteins with altered binding properties
8. Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli
9. “Western blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
10. A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals
11. The behavior of antigens and antibodies immobilized on a solid phase
12. Nucleotide sequence of the alkaline phosphatase gene of Escherichia coli
13. Protein folding
14. Peptides on phage: a vast library of peptides for identifying ligands
15. Random peptide libraries: a source of specific protein binding molecules
16. High efficiency transformation of E. coli by high voltage electroporation
17. 2
18. Parallel association of Fos and Jun leucine zippers juxtaposes DNA binding domains
19. The use of biotinylated monoclonal antibodies and streptavidin affinity chromatography to isolate herpesvirus hydrophobic proteins or glycoproteins
20. 2in vitro
21. Identification of clones that encode chicken tropomyosin by direct immunological screening of a cDNA expression library
22. Pharmaceutical screening: from off-the-wall to off-theshelf
23. Use of monoclonal antibodies to detect conformational alterations in lactate dehydrogenase isoenzyme 5 on heat denaturation and on adsorption to polystyrene plates
24. A rapid boiling method for the preparation of bacterial plasmids
25. T-cell antigen receptors and the immunoglobulin supergene family
26. Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains
27. Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda
28. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli
29. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces
30. Antibody redesign by chain shuffling from random combinatorial immunoglobulin libraries
31. Transcription factor interactions: basics on zippers
32. The role of the leucine zipper in the fosjun interaction
33. By-passing immunization: building high affinity human antibodies by chain shuffling
34. Gene isolation by screening Agtil libraries with antibodies
35. Phage antibodies: filamentous phage displaying antibody variable domains
36. Cloning and expression of recombinant Aspergillus fumigatus allergen I/a (rAsp fI/a) with IgE binding and type I skin test activity
37. Design of DNA-binding peptides based on the leucine zipper motif
38. Preferential heterodimer formation by isolated leucine zippers from Fos and Jun
39. Antibody-selectable filamentous fd phage vectors: affinity purification of target genes
40. Molecular dissection of protein antigens
41. Tricine-sodium dodecyl sulfatepolyacrilamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
42. Searching for peptide ligands with an epitope library
43. λZap: an expression vector with in vivo excision properties
44. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface
45. Surface display and peptide libraries
46. Agtil: gene isolation with antibody probes and other applications
47. The immunochemistry of sandwich ELISAs, II. A novel system prevents denaturation of capture antibodies
48. Immunoblotting with monoclonal antibodies: loss of immunoreactivity with human immunoglobulins arises from polypeptide chain separation
49. Leucine repeats and an adjacent DNA binding domains mediate the formation of functional cFos-cJun heterodimeres
50. β-galactosidase from termination and deletion mutant strains