Cloning, expression and characterisation of the cDNA encoding human hepatic squalene synthase, and its relationship to phytoene synthase

Gene

Volumn 136, Issue 1-2, 1993, Pages 185-192

  Summers, Claire ^a   Karst, Francis ^b   Charles, Andrew D ^a  

^a ASTRAZENECA   (United Kingdom)

^b UNIVERSITÉ DE POITIERS   (France)

Author keywords

biosynthesis; cholesterol; lovastatin; PCR; recombinant DNA; transcriptional regulation; Yeast

Indexed keywords

COMPLEMENTARY DNA; PHYTOENE SYNTHASE; SQUALENE SYNTHASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; GENE EXPRESSION; HUMAN; MOLECULAR CLONING; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; ANIMAL; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; HUMAN; LIVER; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RATS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SQUALENE SYNTHETASE; TRANSCRIPTION, GENETIC; TRANSFERASES;

EID: 0027717852     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(93)90462-C     Document Type: Article

References (41)

1. Farnesyl diphosphate synthetase. Molecular cloning, sequence and expression of an essential gene from Saccharomyces cerevisiae
2. Conserved enzymes mediate the early reactions of carotenoid biosynthesis in nonphotosynthetic and photosynthetic prokaryotes
3. Elucidation of the deficiency in two yeast coenzyme Q mutants. Characterization of the structural gene encoding hexaprenyl pyrophosphate synthetase
4. COQ2 is a candidate for the structural gene encoding para-hydroxybenzoate:polyprenyltransferase
5. A tomato gene expressed during fruit ripening encodes an enzyme of the carotenoid biosynthesis pathway
6. Structural and functional conservation between yeast and human 3-hydroxy-3-methylglutaryl coenzyme A reductases, the rate limiting enzyme of sterol biosynthesis
7. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-protein interactions
8. The Neurospora crassa carotenoid biosynthetic gene (Albino 3) reveals highly conserved regions among prenyltransferases
9. Molecular cloning and expression in Escherichia coli of a cyanobacterial gene coding for phytoene synthase, a carotenoid biosynthesis enzyme
10. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
11. Protein isoprenylation and methylation at carboxy-terminal cysteine residues
12. Regulation of squalene synthetase in human hepatoma cell line Hep G2 by sterols and not by mevalonate-derived non-sterols
13. A comprehensive set of sequence analysis programs for the VAX
14. Carotenoid biosynthesis Isolation and characterization of a bifunctional enzyme catalyzing the synthesis of phytoene
15. Squalene synthetase activity in human fibroblasts: regulation via the low density lipoprotein receptor
16. Isolation and primary structure of the ERG9 gene of Saccharomyces cerevisiae encoding squalene synthetase
17. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family
18. Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli
19. Regulation of the mevalonate pathway
20. Alternatives for the initiation of translation
21. A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors
22. Molecular cloning and characterization of the yeast gene for squalene synthetase
23. Squalene synthetase. Solubilization and partial purification of squalene synthetase, copurification of presqualene pyrophosphate and squalene synthetase activities
24. A simple method for displaying the hydropathic character of a protein
25. Regulation of squalene synthetase and squalene epoxidase activities in Saccharomyces cerevisiae
26. Polyadenylation of mRNA precursors
27. Molecular cloning, expression and characterisation of the cDNA for the rat hepatic squalene synthase
28. Elucidation of the Erwinia-uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia-coli
29. Phosphate binding sequences in nucleotide-binding proteins
30. Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase
31. Squalene synthetase
32. Conversion of farnesyl pyrophosphate to squalene
33. Sequence of pTOM5 a ripening-related cDNA from tomato
34. New functional assignment of the carotenogenic genes crtB and crtE with constructs of these genes from Erwinia species
35. Purification to homogeneity and some properties of squalene synthetase
36. Solubilisation, purification and characterisation of a solubilised form of rat hepatic squalene synthetase
37. The effects of feeding, refeeding, and time of day on the levels of enzymes effecting the conversion of 3-hydroxy-3-methylglutaryl-coenzyme A to squalene
38. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
39. Molecular cloning of mevalonate kinase and regulation of its mRNA levels in rat liver
40. Isolation and sequence of the human farnesyl pyrophosphate synthetase cDNA. Coordinate regulation of the mRNAs for farnesyl pyrophosphate synthetase, 3-hydroxy-3-methylglutaryl coenzyme A reductase and 3-hydroxy-3-methylglutaryl coenzyme A synthase by phorbol ester