Affinity–based reversed micellar protein extraction: I. Principles and protein–ligand systems

Biotechnology and Bioengineering

Volumn 42, Issue 10, 1993, Pages 1199-1208

  Kelley, Brian D ^a   Wang, Daniel I C ^a   Hatton, T Alan ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

affinity partitioning; chymotrypsin; concanavalin A; hydrophilic ligands; myelin basic protein; protein extraction; reversed micelles

Indexed keywords

BIOLOGICAL MEMBRANES; PH EFFECTS; SALTS;

PROTEIN-LIGAND SYSTEMS; REVERSED MICELLES;

PROTEINS;

CHYMOTRYPSIN; CONCANAVALIN A; MYELIN BASIC PROTEIN;

ARTICLE; BINDING AFFINITY; EXTRACTION; MICELLE;

EID: 0027703828     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.260421010     Document Type: Article

References (45)

1. Liquid–liquid extraction for protein separations
2. Selective separation and purification of two lipases from Chromobacterium viscosum using AOT reversed micelles
3. N‐Alkylboronic acids as bifunctional reversible inhibitors of α‐chymotrypsin
4. Interfacial tension minima in oil–water surfactant systems
5. The inhibition of class C beta‐lactamases by boronic acids
6. Hydroboration. XXXIX. 1,3,2‐Benzodioxaborole (catecholborane) as a new hydroboration reagent for alkenes and alkynes. A general synthesis of alkane‐ and alkeneboronic acids and esters via hydroboration. Directive effects in the hydroboration of alkenes and alkynes with catecholborane
7. Alkyl isocyanates as active‐site‐specific reagents for serine proteases. Identification of the active‐site serine as the site of reaction
8. N‐Laurylbiotinamide as affinity cosurfactant
9. Effect of temperature on the reversed micellar extraction of enzymes
10. Enzyme recovery by liquid–liquid extraction using reversed micelles
11. Engineering an enzyme by site‐directed mutagenesis to be resistant to chemical oxidation
12. Sulfonyl fluorides as inhibitors of esterases. I. Rates of reaction with acetylcholinesterase, chymotrypsin and trypsin
13. Boronic acid inhibitors of porcine pancreatic lipase
14. Purification of intracellular enzymes from whole bacterial cells using reversed micelles
15. Liquid–liquid extraction of low molecular weight proteins by selective solubilization in reversed micelles
16. The lectins: Carbohydrate‐binding proteins of plants and animal
17. Interactions between bovine myelin basic protein and zwitterionic lysophospholipids
18. Extraction of proteins and amino acids using reversed micelles
19. Extraction of alphachymotrypsin using reversed micelles
20. Hydrophobized proteins penetrating lipid membranes
21. Affinity‐based reversed micellar protein extraction: II. The effect of the cosurfactant tail length
22. Solubilization and catalysis in reversed micelles
23. Denaturation and deactivation of porcine pancreatic lipase in reversed micellar extraction
24. Catecholborane, a new hydroboration reagent
25. Specific ion effects in electrical double layers: selective solubilization of cations in AOT reversed micelles
26. Application of reverse micelles for the extraction of amino acids and proteins
27. Inhibition of subtilisin by boronic acids, potential analogs of tetrahedral reaction intermediates
28. Solubilization of enzymes and nucleic acids in hydrocarbon micellar solutions
29. Protein extraction by reverse micelles: a study of the factors affecting the forward and backward transfer of chymotrypsin and its activity
30. X‐ray crystallographic study of boronic acid adducts with subtilisin BPN (Novo)
31. An analysis of the regions of the myelin basic protein that bind to phosphatidylcholine
32. Purification of glycoproteins by selective transport using concanavalin‐mediated reverse micellar extraction
33. Recovery of extracellular alkaline protease from whole fermentation broth using reversed micelles
34. Structural characterization of chymotrypsin‐containing AOT reversed micelles
35. Selectivity of interaction of phospholipids with bovine spinal cord myelin basic protein studied by spin‐label electron spin resonance
36. A polypeptide fusion designed for the purification of recombinant proteins
37. Small‐angle neutron scattering studies of protein‐reversed micelle complexes
38. Protein–carbohydrate interaction. IX. Application of the quantitative hapten inhibition technique to polysaccharide–concanavalin A interaction. Some comments on the forces involved in concanavalin A–polysaccharide interaction
39. Protein–carbohydrate interaction. XX. On the number of binding sites on concanavalin‐A, the phytohemagglutinin of the jack bean
40. Dimensional mapping of the active site of cholesterol esterase with alkylboronic acid inhibitors
41. Protein transfer from an aqueous phase into reversed micelles: the effect of protein size and charge distribution
42. Protein separations using reversed micelles
43. Bioaffinity separations using reversed micellar extraction