An SAR sequence containing 395 bp DNA fragment mediates enhanced, gene-dosage-correlated expression of a chimaeric heat shock gene in transgenic tobacco plants

Transgenic Research

Volumn 2, Issue 2, 1993, Pages 93-100

  Schöffl, Fritz ^a   Schröder, Gerald ^a   Kliem, Manfred ^a   Rieping, Mechthild ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

gene dosage; GUS reporter; heat shock; nuclear scaffold; SAR sequences; tobacco

Indexed keywords

BETA GLUCURONIDASE; DNA;

ARTICLE; BINDING SITE; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC TRANSFORMATION; GENETICS; HEAT; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLANT; SOYBEAN; TOBACCO; TRANSGENIC PLANT;

BASE SEQUENCE; BINDING SITES; DNA; GENE AMPLIFICATION; GENE EXPRESSION; GLUCURONIDASE; HEAT; MOLECULAR SEQUENCE DATA; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; SOYBEANS; SUPPORT, NON-U.S. GOV'T; TOBACCO; TRANSFORMATION, GENETIC;

EID: 0027564737     PISSN: 09628819     EISSN: 15739368     Source Type: Journal    
DOI: 10.1007/BF01969382     Document Type: Article

References (45)

1. Functional analysis of sequences required for transcriptional activation of a soybean heat shock gene in transgenic tobacco
2. Expression of a Vicia faba legumin B gene in transgenic tobacco plants: gene dosage-dependent protein accumulation
3. Upstream sequences regulating legumin gene expression in heterologous transgenic plants
4. Chromatin domain surrounding the human interferon-β gene as defined by scaffold-attachment regions
5. Expression of the β-subunit of β-conglycinin in seeds of transgenic plants
6. Characterization of a plant scaffold attachment region in a DNA fragment that normalizes transgene expression in tobacco
7. Regulated expression of genes encoding soybean β-conglycinin in transgenic plants
8. Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites
9. Regulatory domains of Gmhsp 17.5-L heat shock promoter of soybean
10. A glimpse at chromosomal order
11. Cohabitation of scaffold binding regions with upstream/enhancer elements of three developmentally regulated genes of D. melanogaster
12. Nuclear scaffolds and scaffold-attachment regions in higher plants
13. Inheritance of functional foreign genes in plants
14. A simple and general method for transferring genes into plants
15. Organization beyonnd the gene
16. A gentle method for preparing cyto- and nucleo-skeletons and associated chromatin
17. Assaying chimeric genes in plants: the GUS gene fusion system
18. Altered gene expression in plants due to trans interactions between homologous genes
19. Scaffold-attached regions from the human interferon β domain can be used to enhance the stable expression of genes under the control of various promoters
20. Flavonoid genes in Petunia: addition of a limited number of gene copies may lead to a suppression of gene expression
21. Scaffold-associated regions: cis-acting determinants of chromatin structural loops and functional domains
22. Epigenetic changes in the expression of the maize A1 gene in Petunia hybrida: role of numbers of integrated gene copies and state of methylation
23. On a test of whether one of two random variables is stochastically larger than the other
24. Gene interactions and epigenetic variation in transgenic plants
25. Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants
26. Hierarchical binding of DNA fragments derived from scaffold-attachment regions: correlation of properties in vitro and function in vivo
27. Organization of the higher order chromatin loop: specific DNA attachment sites on nuclear scaffold
28. Introduction of a chimaeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans
29. Heat shock and other stress response systems of plants
30. Pea convicilin: structure and primary sequence of the protein and expression of a gene in the seeds of transgenic tobacco
31. The chicken lysozyme 5′ matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes
32. Nucleotide sequence analysis of soyabean small heat shock protein genes belonging to two different multigene families
33. Synergistic effect of upstream sequences, CCAAT box elements, and HSE sequences for enhanced expression of chimaeric heat shock genes in transgenic tobacco
34. Silencing: the establishment and inheritance of stable, repressed transcription states
35. Genetic engineering strategies for manipulation of the heat shock response
36. Thermoinduced transcripts of a soyabean heat shock gene after transfer into sunflower using a Ti plasmid vector
37. Regulation of the transcription of heat shock genes in nuclei from soybean (Glycine max) seedlings
38. The function of plant heat shock promoter elements in the regulated expression of chimaeric genes in transgenic tobacco
39. Functional analysis of sequences regulating the expression of heat shock genes in transgenic plants
40. The induction of the heat shock response: activation and expression of chimaeric heat shock genes in transgenic plants
41. The heat shock response in transgenic plants: the use of chimaeric heat shock genes
42. A scaffold-associated DNA region is located downstream of the pea plastocyanin gené
43. A nuclear DNA attachment element mediates elevated and position-independent gene activity