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Volumn 259, Issue 5095, 1993, Pages 686-688

Selection of bacterial virulence genes that are specifically induced in host tissues

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0027497926     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.8430319     Document Type: Article
Times cited : (564)

References (28)
  • 4
    • 85034937165 scopus 로고    scopus 로고
    • note
    • - strain of S. typhimurium, MT168 [DEL2901 (purA874::IS10)].
  • 6
    • 85034938722 scopus 로고    scopus 로고
    • note
    • A phage P22 lysate grown on the integrated fusion strain was used to transduce a recipient that contains the Pi replication protein, which is required for replication of the plasmid. The transducing phage delivers a linear chromosomal fragment that contains the integrated fusion plasmid. Presumably, once the chromosomal fragment enters the recipient cell, the plasmid circularizes by homologous recombination between the duplicated region defined by the cloned S. typhimurium DNA. The circularized fragment can then replicate as a plasmid in the presence of the Pi replication protein, resulting in the cloned fusion of interest.
  • 26
    • 85034938618 scopus 로고    scopus 로고
    • note
    • 5 cells of an individual fusion strain. Six days after infection, the mice were killed and their spleens were removed. The β-gal activity in each sample was determined by a kinetic assay with the use of the fluorescent substrate fluorescein di-β-o-galactopyranoside (FDG; Molecular Probes, Inc.) and a model SPF-500c spectrofluorometer (SLM Aminco). The units of β-gal were obtained by comparison of the activity to a standard curve.
  • 28
    • 85034941228 scopus 로고    scopus 로고
    • note
    • We thank P. Hanna and J. Tobias for critically reading the manuscript. Supported by National Institutes of Health grant AI26289 (to J.J.M.), National Research Service Award AI08245 (to M.J.M.), and Damon Runyon-Walter Winchell Cancer Research Fund DRG-1016 (to J.M.S.).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.