Peptide mapping analysis of group I allergens of grass pollens

International Archives of Allergy and Immunology

Volumn 102, Issue 2, 1993, Pages 144-151

  Suphioglu, Cenk ^a   Singh, Mohan B ^a   Bruce Knox, R ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Cyanogen bromide digestion; Grass pollen allergens; Group I allergens; IgE binding; Peptide mapping; Protein purification; Proteolysis

Indexed keywords

CYANOGEN BROMIDE; IMMUNOGLOBULIN E; MONOCLONAL ANTIBODY; POLLEN ANTIGEN;

AMINO ACID SEQUENCE; ANTIGENICITY; ARTICLE; BINDING AFFINITY; COMPARATIVE STUDY; GRASS; GRASS POLLEN; IMMUNOBLOTTING; NONHUMAN; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION; SILVER STAINING;

ALLERGENS; COMPARATIVE STUDY; CYANOGEN BROMIDE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITOPES; HUMAN; HYPERSENSITIVITY; IMMUNOGLOBULIN E; PEPTIDE HYDROLASES; PEPTIDE MAPPING; POACEAE; POLLEN; SUPPORT, NON-U.S. GOV'T;

EID: 0027490024     PISSN: 10182438     EISSN: 14230097     Source Type: Journal    
DOI: 10.1159/000236565     Document Type: Article

References (28)

1. Haas H, Becker WM, Maasch HJ, Schklaak M: Analysis of allergen components in grass pollen extracts using immunoblotting. Int Arch Allergy Appl Immunol 1986;79:434-440.
2. Wuthrich B: Epidemiology of the allergic diseases: Are they really on the increase? Int Arch Allergy Appl Immunol 1989;90:3-10.
3. Marsh DG, Goodfriend L, King TP, Lowenstein H, Platts-Mills TAE: Allergen nomenclature. J Allergy Clin Immunol 1987;80:639-645.
4. Sutton R, Wrigley CW, Baldo BA: Detection of IgE- and IgG-binding proteins alter electrophoretic transfer from polyacrylamide gels. J Immunol Methods 1982;52:183-194.
5. Aas K: Clinical and experimental aspects of standardisation and purification of allergen. Int Arch Allergy Appl Immmunol 1975;49:44.
6. Thompson RA, Bousquet J, Cohen S, Frei PC, Jager I, Lambert PH, Lessof MH, Loblay RH, Mailing HJ, Norman S, de Weck Al. Wecke B: The current status of allergen immunotherapy (hyposensitization). Allergy 1989;44:369-379.
7. Esch RE, Klapper DG: Identification and localization of allergenic determinants on grass group I antigens using monoclonal antibodies. J Immunol 1989;142:179-184.
8. Martin BG, Mansfield LE, Nelson HS: Crossallergenicity among the grasses. Ann Allergy 1985;54:54-99.
9. Singh MB, Knox RB: Grass pollen allergens: Antigenic relationships detected using monoclonal antibodies and dot blotting immunoassay. Int Arch Allergy Appl Immunol 1985;78: 300-304.
10. Cleveland DW, Fischer SG, Kirschner MW, Laemmli UK: Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem 1977; 252:1102-1106.
11. Fischer SG: Peptide mapping in gels: analytical methods for gene products. Methods in Enzymology 1983;100:424-430.
12. Crimmins DL, Thoma RS, McCourt DW, Schwartz BD: Strong-cation-exchange sulfocthyl aspartamide chromatography for peptide mapping of Staphylococcus aureus V8 protein digests. Anal Biochem 1989;176:255-260.
13. Houen G, Sando T: Peptide mapping in sodium dodecyl sulfate-containing buffers: Control of proteolytic cleavage by organic solvent. Anal Biochem 1991;193:186-190.
14. Olsen E, Zhang L, Hill RD, Kisil FT, Sehon AH, Mohapatra SS: Identification and characterization of the Poa p IX group of basic allergens of Kentucky bluegrass pollen. J Immuno 1991;147:205-211.
15. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochcm 1976;72:248-254.
16. Laemmli UK: Cleavage of structural proteins during assembly of the head of the bacteriophage T4. Nature 1970;227:680-685.
17. Ansorge W: Fast visualisation of protein bands by impregnation in potassium permanganate and silver nitrate; in Stathakos (ed): Electrophoresis ’82. New York, Walter de Gruyter. 1983. pp 235-242.
18. Towhin H, Gordon J: Immunoblotting and dot immunobinding: Current status and outlook. J Immunol Methods 1984;72:313-340.
19. Simpson RJ, Reid GE, Moritz RL, Morton C, Norton RS: Complete amino acid sequence of tenebrosin-C, a cardiac stimulatory and haemolytic protein from the sea anemone Actinia tenebrosa. F, ur J Biochcm 1990;190:319-328.
20. Smart IJ, Heddle RJ, Zola H, Bradley J: Development of monoclonal mouse antibodies specific for allergenic components in rye grass (Lolium perenne) pollen. Int Arch Allergy Appl Immunol 1983;72:243-248.
21. Singh MB, Hough T, Theerakulpisut P, Avjioglu A, Davies S, Smith PM, Taylor P, Simpson RJ, Ward LD, McCluskey J, Puy R, Knox RB: Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: Intracellular targeting to the amyloplast. Proc Natl Acad Sci USA 1991;88:1384-1388.
22. Ong EK, Suphioglu C, Singh MB, Knox RB: Immunodetection methods for grass pollen allergens on Western blots. Int Arch Allergy Appl Immunol 1990;93:338-343.
23. Ladigss PY, Prober S, Nelson G: Cladistic and biogeographic analysis of the ‘blue ash’ eucalypts. Cladistics. in press.
24. Gonzales RM, Cortes C, Conde J, Negro JM, Rodriguez J, Tursi A, Wutrich B, Carreira J: Cross-reactivity among five major pollen allergens. Ann Allergy 1987;59:149-154.
25. Ford SA, Tovey ER, Baldo BA: Identification of orchard grass (Oactylisglomcrata) pollen allergens following electrophoretic transfer to nitrocellulose. Int Arch Allergy Appl Immunol 1985;78:15-21.
26. Ford SA, Baldo BA: Identification of Bermuda grass (Cynodon dactyhn) pollen allergens by electrablotting. J Allergy Clin Immunol 1987; 79:711-720.
27. Shen H-D, Wang SR, Tang RB, Chang SN, Su SN, Han SH: Identification of allergens and antigens of Bermuda grass (Cynodon dactyhn) pollen by immunoblot analysis. Clin Allergy 1988;18:401-409.
28. Singh MB, Smith PM, Knox RB: Molecular biology of rye-grass pollen allergens; in Baldo BA (ed): Molecular Approaches to the Study of Allergens. Monogr Allergy. Karger. Basel. 1990. vol 28. pp 101-120.