Differential localization of the 5a-reductase and the 3a-hydroxysteroid dehydrogenase in neuronal and glial cultures

Endocrinology

Volumn 132, Issue 3, 1993, Pages 1252-1259

  Melcangi, R C ^a   Celotti, F ^a   Castano, P ^a   Martini, L ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

3ALPHA HYDROXYSTEROID DEHYDROGENASE; ANDROSTANOLONE; CARBON 14; STEROID 5ALPHA REDUCTASE; TESTOSTERONE;

ANIMAL TISSUE; ARTICLE; ASTROCYTE; EMBRYO; ENZYME ACTIVITY; ENZYME LOCALIZATION; GLIA CELL; IMMUNOFLUORESCENCE MICROSCOPY; NERVE CELL; NEWBORN; NONHUMAN; OLIGODENDROGLIA; PRIORITY JOURNAL; RAT; TESTOSTERONE METABOLISM; TRANSMISSION ELECTRON MICROSCOPY;

3-HYDROXYSTEROID DEHYDROGENASES; ANALYSIS OF VARIANCE; ANIMAL; ANIMALS, NEWBORN; ASTROCYTES; CARBON RADIOISOTOPES; CELLS, CULTURED; CEREBRAL CORTEX; EMBRYO; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GLIAL FIBRILLARY ACIDIC PROTEIN; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON; NEUROGLIA; NEURONS; OLIGODENDROGLIA; ORGANELLES; PREGNANCY; RATS; RATS, SPRAGUE-DAWLEY; STANOLONE; SUPPORT, NON-U.S. GOV'T; TESTOSTERONE; TESTOSTERONE 5-ALPHA-REDUCTASE;

EID: 0027479169     PISSN: 00137227     EISSN: 19457170     Source Type: Journal    
DOI: 10.1210/endo.132.3.8440186     Document Type: Article

References (25)

1. Canick JA, Vaccaro DE, Livingston EM, Leeman SE, Ryan KJ, Fox TO1986 Localization of aromatase and 5α-reductase to neuronal and non-neuronal cells in the fetal rat hypothalamus. Brain Res 372:277-282
2. Melcangi RC, Celotti F, Ballabio M, Castano P, Massarelli R, Poletti A, Martini L 1990 5α-Reductase activity in isolated and cultured neuronal and glial cells of the rat. Brain Res 516:229-236
3. Melcangi RC, Celotti F, Ballabio M, Castano P, Poletti A, Milani S, Martini L1988 Ontogenetic development of the 5α-reductase in the rat brain: cerebral cortex, hypothalamus, purified myelin and isolated oligodendrocytes. Dev Brain Res 44:181-188
4. Weidenfeld J, Abramsky O, Ovadia H, Metabolism of gonadal steroids by normal and neoplastic glial cells. 7th International Congress of Endocrinology, Quebec, Quebec, Canada, 1984 (Abstract 2745)
5. Weidenfeld J, Schorr J, Abramsky O, Metabolism of gonadal steroids by oligodendrocytes of calf and rat brain. 12th Annual Meeting of the Society for Neuroscience, Minneapolis, MN, 1982 (Abstract 63.19)
6. Melcangi RC, Celotti F, Castano P, Martini L1992 Intracellular signalling systems controlling the 5α-reductase in glial cell cultures. Brain Res 585:411-415
7. Raff MC, Abney ER, Cohen J, Lindsay R, Noble M1983 Two types of astrocytes in cultures of developing rat white matter: differences in morphology, surface gangliosides, and growth characteristics. J Neurosci 3:1289-1300
8. Raff MC, Miller RH, Noble M1983 A glial progenitor cell that develops in vitrointo an astrocyte or an oligodendrocytes depending on culture medium. Nature 303:390-396
9. Wilkin GP, Marriot DR, Cholewinski AJ 1990Astrocyte heterogeneity. Trends Neurosci13:43-45
10. McCarthy KD, De Vellis J1980 Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J Cell Biol85:890-902
11. Aloisi F, Agresti C, Levi G1988 Establishment, characterization, and evolution of cultures enriched in type 2 astrocytes. J Neurosci Res 21:188-198
12. Besnard F, Perraud F, Sensenbrenner M, Labourdette G 1989Effects of acid and basic fibroblast growth factors on proliferation and maturation of cultured rat oligodendrocytes. Int J Dev Neurosci7:401-409
13. Bradford MM 1976A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ann Biochem72:248-254
14. Bunge RP 1968Glial cells and the central myelin sheath. Physiol Rev48:197-248
15. Peters A1964 Observations on the connexions between myelin sheaths and glial cells in the optic nerves of young rats. J Anat98:125-134
16. Melcangi RC, Celotti F, Ballabio M, Poletti A, Castano P, Martini L1988 Testosterone 5α-reductase activity in the rat brain is highly concentrated in white matter structures and in purified myelin sheaths of axons. J Steroid Biochem 31:173-179
17. Celotti F, Melcangi RC, Martini L1992 The 5α-reductase in the brain: molecular aspects and relation to brain function. Front Neu-roendocrinol 13:163-215
18. Melcangi RC, Celotti F, Negri-Cesi P, Castano P, Massarelli R, Poletti A, Martini L, 5α-Reductase and aromatase activity in isolated and cultured neuronal and glial cells of the rat. 72nd Annual Meeting of The Endocrine Society, Atlanta, GA, 1990 (Abstract1288)
19. Negri Cesi P, Melcangi RC, Celotti F, Martini L1992 Aromatase activity in cultured brain cells: difference between neurons and glia. Brain Res 589:327-332
20. Andersson S, Berman DM, Jenkins EP, Russell DW1991 Deletion of steroid 5α-reductase 2 gene in male pseudohermaphroditism. Nature 354:159-161
21. Krieger NR, Scott RG1989 Nonneuronal localization for steroid converting enzyme: 3α-hydroxysteroid oxidoreductase in olfactory tubercle of rat brain. J Neurochem 52:1866-1870
22. Sar M, Stumpf WE1975 Distribution of androgen-concentrating neurons in rat brain. In: Stumpf WE, Grant LD (eds) Anatomical Neuroendocrinology. Karger, Basel, pp 120-133
23. Sheridan PJ 1984Autoradiographic localization of steroid receptors in the brain. Clin Neuropharmacol7:281-295
24. Jung-Testas I, Renoir M, Bugnard H, Greene GL, Baulieu EE 1992 Demonstration of steroid hormone receptors and steroid action in primary cultures of rat glial cells. J Steroid Biochem Mol Biol41:621-631
25. Martini L1982 The 5α-reductase of testosterone in the neuroendocrine structures. Biochemical and physiological implications. En-docr Rev 3:1-25