The Effect of Formalin Fixation on DNA and the Extraction of High-molecular-weight DNA from Fixed and Embedded Tissues

Pathology Research and Practice

Volumn 189, Issue 1, 1993, Pages 66-72

  Koshiba, M ^a   Ogawa, K ^a   Hamazaki, S ^a   Sugiyama, T ^a   Ogawa, O ^a   Kitajima, T ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

DNA analysis; Formalin; High molecular weight DNA; Southern blot analysis

Indexed keywords

DNA; FORMALDEHYDE;

ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; SOUTHERN BLOTTING; TISSUE FIXATION;

EID: 0027478499     PISSN: 03440338     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0344-0338(11)80118-4     Document Type: Article

References (23)

1. High molecular weight DNA from fixed cytogenetic preparations
2. The effects of fixative type and fixation time on the quantity and quality of extractable DNA for hybridization studies on lymphoid tissue
3. Analysis of c-Ki-ras mutations in human colon carcinoma by cell sorting, polymerase chain reaction, and DNA sequencing
4. Southern blot analysis of DNA extracted from formalinfixed pathology specimens
5. On the reversibility of the acid denaturation of sodium desoxyribose nucleate
6. Purification of DNA from formaldehyde-fixed and paraffin-embedded human tissue
7. Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides
8. Studies on histone organization in the nucleosome using formaldehyde as a reversible cross-linking agent
9. Extraction and amplification of DNA from archival hematoxylin and eosin sections and cervical cytology Papanicolaou smears
10. Detection of human papillomavirus in formalin-fixed, invasive squamous carcinomas using the polymerase chain reaction
11. Rapid microwave fixation of human tissues for light microscopic immunoperoxi Base identification of diagnostically useful antigens
12. Review of the genotoxicity of formaldehyde
13. Histological fixation by microwave heating
14. Comparison of formalin, buffered formalin, and Bouin's fixation on the detection of human papillomavirus deoxyribonucleic acid from genital lesions
15. Buffered formalin is the superior fixative for the detection of HPV DNA by in situ hybridization analysis
16. Analysis of DNA from cells fixed in Carnoy's solution for cytogenetic study
17. Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction
18. The AMeX method: A multipurpose tissue-processing and paraffin-embedding method: II
19. Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization
20. Analysis of DNA sequences in forty-year-old paraffin-embedded thin-tissue sections: A bridge between molecular biology and classical histology
21. The mild acidic degradation of desoxyribose nucleic acid
22. Fundamental study on the mechanism of DNA degradation in tissues fixed in formaldehyde
23. Gene amplification of c-myc and N-myc in small cell carcinoma of the lung