How sensitive is PCR‐SSCP?

Human Mutation

Volumn 2, Issue 5, 1993, Pages 338-346

  Hayashi, Kenshi ^a   Yandell, David W ^b  

^a KYUSHU UNIVERSITY   (Japan)

^b MASSACHUSETTS EYE AND EAR INFIRMARY   (United States)

Author keywords

DNA testing; Mutational analysis; Polymerase chain reaction (PCR); Single strand conformation polymorphism (SSCP)

Indexed keywords

SINGLE STRANDED DNA;

DIAGNOSTIC ACCURACY; DNA CONFORMATION; DNA POLYMORPHISM; GENE DELETION; GENE INSERTION; GENE MUTATION; GENE REARRANGEMENT; HUMAN; HUMAN CELL; NUCLEIC ACID BASE SUBSTITUTION; ONCOGENE; POLYMERASE CHAIN REACTION; REVIEW; TUMOR SUPPRESSOR GENE;

DNA, SINGLE-STRANDED; HUMAN; NUCLEIC ACID CONFORMATION; POLYMERASE CHAIN REACTION; POLYMORPHISM (GENETICS); SENSITIVITY AND SPECIFICITY; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0027434131     PISSN: 10597794     EISSN: 10981004     Source Type: Journal    
DOI: 10.1002/humu.1380020503     Document Type: Review

References (42)

1. Diagnostic single strand conformation polymorphism, (SSCP): A simple nonradioisotopic method as applied to a Tay‐Sachs B1 variant
2. A major segment of the neurofibromatosis type I gene: cDNA sequence, genomic structure and point mutations
3. Polyacrylamide gel electrophoresis
4. Detection of point mutations in the p53 gene: comparison of single‐strand conformation polymorphism, constant denaturant gel electrophoresis, and hydroxylamine and osmium tetroxide techniques
5. Reactivity of cytosine and thymine in single‐base‐pair mismatches with hydroxylamine and osmium tetroxide and its application to the study of mutations
6. Detection of point mutations in human DNA by analysis of RNA conformation polymorphism(s)
7. Separation of random fragments of DNA according to properties of their sequences
8. p53 mutations in human lymphoid malignancies: association with Burkitt lymphoma and chronic lymphocytic leukemia
9. Identification and characterization of the familial adenomatous polyposis coli gene
10. Use of labeled primers in polymerase chain reaction (LP‐PCR) for a rapid detection of the product
11. PCR‐SSCP: a simple and sensitive method for detection of mutations in the genomic DNA
12. PCR‐SSCP: a method for detection of mutations
13. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support
14. Rapid detection of ras oncogenes in human tumors: applications to colon, esophageal and gastric cancer
15. Rapid detection of single base mismatches as heteroduplexes on hydrolink gel
16. Detection of mutations in the human insulin gene by single strand conformation polymorphisms
17. p53 Gene alterations and protein accumulation in human ovarian cancer
18. Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis
19. F‐SSCP: fluorescencebased polymerase chain reaction‐single strand conformation polymorphism (PCR‐SSCP) analysis
20. Sequencing end‐labeled DNA with base‐specific chemical cleavages
21. Strand‐separating conformational polymorphism analysis: efficacy of detection of point mutations in the human ornithine δ‐aminotransferase gene
22. Detection of aberrations of the p53 alleles and the gene transcript in human tumor cell lines by single‐stranded conformation polymorphism analysis of polymerase chain reaction products
23. Inactivation of the retinoblastoma gene in a human lung carcinoma cell line detected by single‐strand conformation polymorphism analysis of the polymerase chain reaction product of cDNA
24. Improved double‐stranded DNA sequencing using the linear polymerase chain reaction
25. Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA: DNA duplex
26. Detection and localization of single base changes by denaturing gradient gel electrophoresis
27. Frequent allelic losses and mutations of the p53 gene in human ovarian cancer
28. A rapid and sensitive detection of point mutations and genetic polymorphisms using polymerase chain reaction
29. Transfer RNA: molecular structure, sequence and properties
30. Primer‐directed enzymatic amplification of DNA with thermostable DNA polymerase
31. Screening for mutations by RNA single‐strand conformation polymorphism (rSSCP): comparison with DNA‐SSCP
32. Dideoxy fingerprinting (ddF): a rapid and efficient screen for the presence of mutations
33. Genetic analysis of amplified DNA with immobilized sequence‐specific oligonucleotide probes
34. Attachment of a 40‐base‐pair G+C rich sequence (GC‐clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single‐base changes
35. Detection of ras gene mutations in human lung cancers by single‐strand conformation polymorphism analysis of polymerase chain reaction products
36. Allele‐specific polymerase chain reaction: a method for amplification and sequence determination of a single component among a mixture of sequence variants
37. Mutation spectrum of the p53 gene in bone and soft tissue sarcomas
38. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β‐globin DNA
39. Detecting single base substitutions as heteroduplex polymorphisms
40. A method to detect and characterize point mutations in transcribed genes: amplification and overexpression and of the mutant c‐K‐ras allele in human tumor cells
41. Quinolone resistant mutations of the gyrA gene of Escherichia coli