Regulation of human liver cytochromes P‐450 in family 3A in primary and continuous culture of human hepatocytes

Hepatology

Volumn 18, Issue 5, 1993, Pages 1254-1262

  Schuetz, Erin G ^a   Schuetz, John D ^a   Strom, Stephen C ^b,c   Thompson, Melissa T ^b   Fisher, Robert A ^b   Molowa, David T ^b   Li, Donna ^b   Guzelian, Philip S ^b  

^a ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^c UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450; DEXAMETHASONE; MACROLIDE; MEVINOLIN; PHENOBARBITAL;

ARTICLE; DOSE RESPONSE; GENE EXPRESSION; GENETIC POLYMORPHISM; HEP 2 CELL; HEPATOBLASTOMA; HUMAN; HUMAN CELL; LIVER CELL CULTURE; MULTIGENE FAMILY; NORTHERN BLOTTING; PRIORITY JOURNAL; REGULATORY MECHANISM;

CARCINOMA, HEPATOCELLULAR; CELLS, CULTURED; CYTOCHROME P-450 CYP2E1; CYTOCHROME P-450 ENZYME SYSTEM; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMAN; LIVER; LIVER NEOPLASMS; MIXED FUNCTION OXYGENASES; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.; TUMOR CELLS, CULTURED;

EID: 0027427551     PISSN: 02709139     EISSN: 15273350     Source Type: Journal    
DOI: 10.1002/hep.1840180535     Document Type: Article

References (53)

1. The molecular biology of cytochrome P450s
2. Regulation of gene expression in adult rat hepatocytes cultured on a basement membrane matrix
3. Induction of cytochrome P‐450 by glucocorticoids in rat liver. II. Evidence that glucocorticoids regulate induction of cytochrome P‐450 by a nonclassical receptor mechanism
4. Separation, purification, and characterization of a novel form of hepatic cytochrome P‐450 from rats treated with pregnenolone‐16 alpha‐carbonitrile
5. Immunochemical evidence for induction of a common form of hepatic cytochrome P‐450 in rats treated with pregnenolone‐16‐alpha‐carbonitrile or other steroidal or nonsteroidal agents
6. Coinduction of multiple hepatic cytochrome P‐450 proteins and their mRNAs in rats treated with imidazole antimycotic agents
7. Identification of the cytochrome P‐450 induced by macrolide antibiotics in rat liver as the glucocorticoid responsive cytochrome P‐450p
8. Complete cDNA sequence of a cytochrome P‐450 inducible by glucocorticoids in human liver
9. Isolation and sequence determination of a cDNA clone related to human cytochrome P‐450 nifedipine oxidase
10. Human P450PCN1: sequence, chromosome localization, and evidence through cDNA expression that P450PCN1 is nifedipine oxidase
11. Complete cDNA and protein sequence of a pregnenolone 16 alpha‐carbonitrile‐induced cytochrome P‐450: a representative of a new gene family
12. Cloning of a cDNA coding for P‐450 LM3c from rabbit liver microsomes and regulation of its expression
13. Characterization of a cDNA encoding a new member of the glucocorticoid‐responsive cytochromes P450 in human liver
14. Cytochrome P‐450 hPCN3, a novel cytochrome P‐450 IIIA gene product that is differentially expressed in adult human liver
15. Identification of a polymorphically expressed member of the human cytochrome P‐450III family
16. Identification of a cytochrome P‐450 in human fetal liver related to glucocorticoid‐inducible cytochrome P‐450HLp in the adult
17. Isolation and characterization of human fetal liver cytochrome P450HLp2: a third member of the P450III gene family
18. P‐450 HFLa, a form of cytochrome P‐450 purified from human fetal livers, is the 16‐alpha‐hydroxylase of dehydroepiandrosterone 3‐sulfate
19. Identification of an inducible form of cytochrome P‐450 in human liver
20. HepG2 cells as a resource for metabolic studies: lipoprotein, cholesterol and bile acids
21. Characteristics of a cell line (TONG/HCC) established from a human hepatocellular carcinoma
22. Characterization of mRNA species related to human liver cytochrome P‐450 nifedipine oxidase and the regulation of catalytic activity
23. Genetic toxicology studies with human hepatocytes
24. Isolation, culture and transplantation of human hepatocytes
25. Regulation of cytochrome P‐450p by phenobarbital and phenobarbital‐like inducers in adult rat hepatocytes in primary monolayer culture and in vivo
26. Monoclonal antibodies against human liver cytochrome P‐450
27. Studies on the expression and metabolic capabilities of human liver cytochrome P450IIIA5 (HLp3)
28. Induction of cytochrome P‐450 by glucocorticoids in rat liver. I. Evidence that glucocorticoids and pregnenolone 16 alpha‐carbonitrile regulate de novo synthesis of a common form of cytochrome P‐450 in cultures of adult rat hepatocytes and in the liver in vivo
29. Induction of cytochrome P‐450 by pregnenolone‐16 alpha‐carbonitrile in primary monolayer cultures of adult rat hepatocytes and in a cell‐free translation system
30. Transcriptional regulation of cytochrome P450b/e and P450p gene expression by growth hormone in adult rat hepatocytes cultured on a reconstituted basement membrane
31. Difference in the susceptibility of two phenobarbital‐inducible forms, P4502B1 and P4502B2, to thyroid hormone‐ and growth hormone‐induced suppression in rat liver: phenobarbital‐inducible 2B2 suppression by thyroid hormone acting directly, but not through the pituitary system
32. Metabolic activation of benzo[a]pyrene by a human hepatoma cell line
33. Mixed function oxidase and UDP‐glucuronyltransferase activities in the human HepG2 hepatoma cell line
34. Drug metabolism by the human hepatoma cell, HepG2
35. Use of the human liver cell ine HepG2 in a modified salmonella reversion assay
36. Induction of drug metabolizing enzymes in human liver cell line HepG2
37. delta‐aminolaevulinate synthase in human HepG2 hepatoma cells
38. Screening for inducers and inhibitors of cytochrome P‐450 (cyclosporin A oxidase) in primary cultures of human hepatocytes and in liver microsomes
39. Maintenance of cytochrome P‐450 in cultured adult human hepatocytes
40. Immunochemical studies for the presence of P‐450 HFLa, a form of cytochrome P‐450, in human fetal livers in human hepatocellular carcinoma cells
41. Identification of glucocorticoid‐inducible cytochromes P‐450 in the intestinal mucosa of rats and man
42. Biotransformation of lovastatin. IV. Identification of cytochrome P450 3A proteins as the major enzymes responsible for the oxidative metabolism of lovastatin in rat and human liver microsomes
43. Effect of 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase inhibitor pravastatin on urinary 6beta‐hydroxycortisol excretion: a preliminary study
44. The increase in urinary excretion of 6β‐hydroxycortisol as a marker of human hepatic cytochrome P450IIIA induction
45. Urinary excretion of 6 beta‐hydroxycortisol in states of altered thyroid function
46. The erythromycin breath test as an assay of glucocorticoid‐inducible liver cytochromes P‐450
47. Interindividual variability and induction of cytochromes P‐450 and UDP‐glucuronosyl transferases in human liver microsomes and primary cultures of human hepatocytes
48. Expression of cytochrome P‐450 enzymes in cultured human hepatocytes
49. Induction, regulation and messenger half‐life of cytochromes P450 IA1, IA2 and IIIA6 in primary cultures of rabbit hepatocytes; ‐CYP 1A1, 1A2 and 3A6 chromosome location in the rabbit and evidence that post‐transcriptional control of gene IA2 does not involve mRNA stabilization
50. Regulation of the rabbit cytochrome P‐450 3c gene
51. Demonstration in multiple species of inducible hepatic cytochromes P‐450 and their mRNAs related to the glucocorticoid‐inducible cytochrome P‐450 of the rat
52. Pituitary regulation of the malespecific steroid 6‐beta‐hydroxylase P‐450 2a (gene product IIIA2) in adult rat liver: suppressive influence of growth hormone and thyroxine acting at a pretranslational level
53. The P450 superfamily: update on new sequences, gene mapping, and recommended nomenclature