Ultra‐rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase‐polymerase chain reaction

Journal of Medical Virology

Volumn 40, Issue 2, 1993, Pages 142-145

  Chungue, Eliane ^a   Roche, Claudine ^a   Lefevre, Marie‐France ^a   Barbazan, P ^b   Chanteau, Suzanne ^a  

^a Institut Territorial de Recherches Medicales Louis Malarde   (French Polynesia)

^b Institut de Recherche pour le Developpement Papeete   (French Polynesia)

Author keywords

dengue diagnosis; genomic amplification; guanidinium extraction; RNA isolation; silica RT PCR

Indexed keywords

ARTICLE; DENGUE; DENGUE VIRUS; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; MOSQUITO; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SERUM; VIRUS ISOLATION;

AEDES; ANIMAL; DENGUE; DENGUE VIRUS; FEMALE; GUANIDINES; HUMAN; POLYMERASE CHAIN REACTION; RNA, VIRAL; RNA-DIRECTED DNA POLYMERASE; SENSITIVITY AND SPECIFICITY; SILICON DIOXIDE; THIOCYANATES;

EID: 0027324667     PISSN: 01466615     EISSN: 10969071     Source Type: Journal    
DOI: 10.1002/jmv.1890400211     Document Type: Article

References (15)

1. The larval habitates of Aedes polynesiensis marks in Tahiti and methods of control
2. Rapid and simple method for purification of nucleic acids
3. Dengue 1 epidemic in French Polynesia, 1988–1989: Surveillance and clinical, epidemiological, virological and serological findings in 1752 documented clinical cases
4. Identification of dengue sequences by genomic amplification: Rapid diagnosis of dengue virus serotypes in peripheral blood
5. Detection of amplified Wuchereria bancrofti DNA in mosquitoes with a non radioactive probe
6. Dengue fever and dengue shock syndrome in French Polynesia
7. Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses
8. Pathogenesis of dengue: Challenges to molecular biology
9. Rapid identification of dengue virus isolates by using monoclonal antibodies in an indirect immunofluorescent assay
10. The dengue viruses
11. Sensitivity and specificity of a universal primer set for the rapid diagnosis of dengue virus infections by polymerase chain reaction and nucleic acid hybridization
12. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase‐polymerase chain reaction
13. Onchocerca volvulus: Application of the polymerase chain reaction to identification and strain differentiation of the parasite
14. A simple method of estimating fifty percent end points
15. Primer‐directed enzymatic amplification of DNA with a thermostable DNA polymerase