Thiol-disulfide effects on hepatic glutathione transport studies in cultured rat hepatocytes and perfused livers

Journal of Clinical Investigation

Volumn 92, Issue 3, 1993, Pages 1188-1197

  Lu, Shelly C ^a   Ge, Jun Li ^a   Huang, Hai Ying ^a   Kuhlenkamp, John ^a   Kaplowitz, Neil ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

BSP GSH; Cystine; DTT; Sinusoidal GSH efflux; Sulfhydryls

Indexed keywords

ADENOSINE TRIPHOSPHATE; CYSTINE; GLUTATHIONE; THIOL DERIVATIVE;

AMINO ACID TRANSPORT; ANIMAL CELL; ARTICLE; DOSE RESPONSE; KINETICS; LIVER CELL; LIVER PERFUSION; MALE; MEMBRANE DEPOLARIZATION; MEMBRANE POTENTIAL; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; RAT; SIGNAL TRANSDUCTION;

ADENOSINE TRIPHOSPHATE; ANIMAL; BIOLOGICAL TRANSPORT; CELLS, CULTURED; DISULFIDES; GLUTATHIONE; IN VITRO; KINETICS; LIVER; MALE; MEMBRANE POTENTIALS; OUABAIN; RATS; RATS, SPRAGUE-DAWLEY; SIGNAL TRANSDUCTION; SULFHYDRYL COMPOUNDS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0027320633     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI116689     Document Type: Article

References (35)

1. Kaplowitz, N., T. K. Aw, and M. Ookhtens. 1985. The regulation of hepatic glutathione. Annu. Rev. Pharmacol. Toxicol. 25:715-744.
2. Meister, A., and M. E. Anderson. 1983. Glutathione. Annu. Rev. Biochem. 52:711-760.
3. Lauterburg, B. H., J. D. Adams, and J. R. Mitchell. 1984. Hepatic glutathione homeostasis in the rat: efflux accounts for glutathione turnover. Hepatology. 4:586-590.
4. Fernandez-Checa, J. C., C. Garcia-Ruiz, M. Ookhtens, and N. Kaplowitz. 1991. Impaired uptake of glutathione by hepatic mitochondria from chronic ethanol-fed rats. J. Clin. Invest. 87:397-405.
5. Ookhtens, M., K. Hobdy, M. C. Corvasce, T. Y. Aw, and N. Kaplowitz. 1985. Sinusoidal efflux of glutathione in the perfused rat liver: evidence for a carrier-mediated process. J. Clin. Invest. 75:258-265.
6. Aw, T. K., M. Ookhtens, C. Ren, and N. Kaplowitz. 1986. Kinetics of glutathione efflux from isolated rat hepatocytes. Am. J. Physiol. 250:G236-G243.
7. Ookhtens, M., I. Lyon, J. Fernandez-Checa, and N. Kaplowitz. 1988. Inhibition of glutathione efflux in the perfused rat liver and isolated hepatocytes by organic anions and bilirubin. Kinetics, sidedness, and molecular forms. J Clin. Invest. 82:608-616.
8. Aw, T. K., M. Ookhtens, and N. Kaplowitz. 1984. Inhibition of glutathione efflux from isolated rat hepatocytes by methionine. J. Biol. Chem. 259:9355-9358.
9. Fernandez-Checa, J. C., C. Ren, T. K. Aw, M. Ookhtens, and N. Kaplowitz. 1988. Effect of membrane potential and cellular ATP on glutathione efflux from isolated rat hepatocytes. Am. J. Physiol. 255:G403-G408.
10. Lu, S., C. Garcia-Ruiz, J. Kuhlenkamp, M. Ookhtens, M. Salas-Prato, and N. Kaplowitz. 1990. Hormonal regulation of GSH efflux. J. Biol. Chem. 265:16088-16095.
11. Garcia-Ruiz, C., J. C. Fernandez-Checa, and N. Kaplowitz. 1992. Bidirectional plasma membrane transport of reduced glutathione in intact hepatocytes and membrane vesicles. J. Biol. Chem. 267:22256-22264.
12. Fernandez-Checa, J. C., Y. R. Yi, C. Garcia-Ruiz, Z. Knezic, S. M. Tahara, and N. Kaplowitz. 1993. Expression of rat liver sinusoidal and canalicular GSH transport systems in xenopus laevis oocytes. J. Biol. Chem. 268:2324-2328.
13. Eriksson, B., and S. A. Eriksson. 1967. Synthesis and characterization of the L-cysteine-glutathione mixed disulfide. Acta. Chem. Scand. 21:1304-1312.
14. Moldeus, P., J. Hogberg, and S. Orrenius. 1978. Isolation and use of liver cells. Methods Enzymol. 51:60-70.
15. Tietze, F. 1969. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem. 27:502-522.
16. Fariss, M. W., and D. J. Reed. 1987. High-performance liquid chromatography of thiols and disulfides: dinitrophenol derivatives. Methods Enzymol. 143:101-109.
17. Booth, J. B., E. Boyland, and P. Sims. 1961. An enzyme from rat liver catalyzing conjugations with glutathione. Biochem. J. 79:516-524.
18. Ballatori, N., and A. T. Truong. 1989. Altered hepatic junctional permeability, bile acid excretion and glutathione efflux during oxidant challenge. J. Pharmacol. Exp. Ther. 251:1069-1075.
19. Ookhtens, M., and T. Maddatu. 1991. Mechanism of changes in hepatic sinusoidal and biliary glutathione efflux with age in rats. Am. J. Physiol. 261:G648-G656.
20. Van Dyke, R. W., and B. F. Scharschmidt. 1983. (Na,K)-ATPase-mediated cation pumping in cultured rat hepatocytes. J. Biol. Chem. 258:12912-12919.
21. Jones, D. P. 1981. Determination of pyridine nucleotides in cell extracts by high-performance liquid chromatography. J. Chromatogr. 225:446-449.
22. Berman, M., and M. F. Weiss. 1977. SAAM Manual. Publication No. 76-730. Department of Health, Education and Welfare, National Institutes of Health, Government Printing Office, Washington, DC.
23. Lash, L. H., and D. P. Jones. 1985. Distribution of oxidized and reduced forms of glutathione and cysteine in rat plasma. Arch. Biochem. Biophys. 240:583-592.
24. Takada, A., and S. Bannai. 1984. Transport of cystine in isolated rat hepatocytes in primary culture. J. Biol. Chem. 259:2441-2445.
25. Lu, S. C., J. Ge, J. Kuhlenkamp, and N. Kaplowitz. 1992. Insulin and glucocorticoid dependence of hepatic g-glutamylcysteine synthetase and glutathione synthesis in the rat. J. Clin. Invest. 90:524-532.
26. Salmon, R. F., and M. Baum, 1990. Intracellular cystine loading inhibits transport in the rabbit proximal convoluted tubule. J. Clin. Invest. 85:340-344.
27. Coor, C., R. F. Salmon, R. Quigley, D. Marver, and M. Baum. 1991. Role of adenosine triphosphate (ATP) and NAK ATPase in the inhibition of proximal tubule transport with intracellular cystine loading. J Clin. Invest. 87:955-961.
28. +-dependent amino acid transport by dibutyryl cyclic AMP in hepatocytes. Biochem. J. 241:737-743.
29. Fariss, M. W., and D. J. Reed. 1983. Measurement of glutathione and glutathione disulfide efflux from isolated rat hepatocytes. In Isolation, Characterization, and Use of Hepatocytes. R. A. Harris and N. W. Cornell, editors. Elsevier Science Publishing Co. 349-355.
30. McAbee, D. D., M. C. Lear, and P. H. Weigel. 1991. Total cellular activity and distribution of a subpopulation of galactosyl receptors in isolated rat hepatocytes are differentially affected by microtubule drugs, monensin, low temperature, and chloroquine. J. Cell. Biochem. 45:59-68.
31. Eilers, U., J. Klumperman, and H. Hauri. 1989. Nocodazole, a microtubule-active drug, interferes with apical protein delivery in cultured intestinal epithelial cells (Caco-2). J. Cell Biol. 108:13-22.
32. Lu, S., J. Kuhlenkamp, C. Garcia-Ruiz, and N. Kaplowitz. 1991. Hormone-mediated down-regulation of hepatic glutathione synthesis in the rat. J. Clin. Invest. 88:260-269.
33. Glazenburg, E. J., J. E. Bruggink, K. Wolters-Keulemans, and G. J. Mulder. 1984. Inhibition of glutathione efflux in the recirculating rat liver perfusion by cysteine but not by oxothiazolidine carboxylate, an intracellular cysteine precursor. FEBS (Fed. Eur Biochem. Soc.) Lett. 178:271-274.
34. Hahn, R., and W. Oberrauch. 1978. Unidirectional transport of reduced glutathione in rat liver and its metabolization in the extracellular space. In Functions of Glutathione in Liver and Kidney. H. Sies and A. Wendel, editors. Springer-Verlag, Berlin. 32-40.
35. Berleth, E. S., J. Li, J. A. Braunscheidel, and C. M. Pickart. 1992. A reactive nucleophile proximal to vicinal thiols is an evolutionarily conserved feature in the mechanism of arg aminoacyl-tRNA protein transferase. Arch. Biochem. Biophys. 298:498-504.