Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins

Analytical Biochemistry

Volumn 210, Issue 1, 1993, Pages 179-187

  Frangioni, John V ^a   Neel, Benjamin G ^a  

^a BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; PEPTIDES; PHOSPHATASES; SCALES (WEIGHING INSTRUMENTS);

ENZYMATIC ACTIVITIES; EUKARYOTIC CELLS; GLUTATHIONE S-TRANSFERASES; HIGH LEVEL EXPRESSION; PROTEIN-TYROSINE PHOSPHATASE; PURIFICATION PROCESS; RAPID PURIFICATION; TYROSINE PHOSPHATASE;

PURIFICATION;

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN;

ARTICLE; BACTERIUM; ENZYME PURIFICATION; NONHUMAN; PRIORITY JOURNAL; SOLUBILIZATION;

ANIMAL; CHROMATOGRAPHY, AFFINITY; EVALUATION STUDIES; GLUTATHIONE; GLUTATHIONE TRANSFERASE; METHODS; PROTEIN-TYROSINE-PHOSPHATASE; RECOMBINANT FUSION PROTEINS; SEPHAROSE; SOLUBILITY; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0027212498     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1006/abio.1993.1170     Document Type: Article

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