Determination of proteins by a reverse biuret method combined with the copper-bathocuproine chelate reaction

Clinica Chimica Acta

Volumn 216, Issue 1-2, 1993, Pages 103-111

  Matsushita, Makoto ^a   Irino, Tsutomu ^a   Komoda, Tsugikazu ^b   Sakagishi, Yoshikatsu ^b  

^a Saitama College   (Japan)

^b SAITAMA MEDICAL UNIVERSITY   (Japan)

Author keywords

Bathocuproine; Chelate reaction; Copper; Protein assay; Reverse biuret method

Indexed keywords

BATHOCUPROINE; COPPER; UNCLASSIFIED DRUG;

ARTICLE; CHELATION; HUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; SERUM;

ASCORBIC ACID; CEREBROSPINAL FLUID PROTEINS; CHELATING AGENTS; COPPER; HUMAN; HYDROGEN-ION CONCENTRATION; INDICATORS AND REAGENTS; PHENANTHROLINES; PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 0027205628     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/0009-8981(93)90143-R     Document Type: Article

References (22)

1. Protein measurement with the Folin phenol reagent
2. Fifty fold amplification of the Lowry protein assay
3. Measurement of protein using bicinchoninic acid
4. Determination of total protein in spinal fluid with sulphosalicylic acid and trichloroacetic acid
5. New micro-turbidimetric method of protein determination in cerebrospinal fluid and urine
6. A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein dye binding
7. Minimization of variation in the response to different proteins of the Coomassie Blue G dye binding assay for protein
8. An improvement of the Coomassie Blue dye binding method allowing an equal sensitivity to various proteins application to cerebrospinal fluid
9. Sensitivity and variability of the Bradford protein assay in the presence of detergents
10. Refinement of the Coomassie Brilliant Blue G assay for quantitative protein determination
11. Urinary protein as measured with a pyrogallol red-molybdate complex manually and in a Hitachi 726 automated analyzer
12. Standards for total serum protein assays A collaborative study
13. Stable nickel/biuret reagent for determination of total proteins in serum
14. Studies in serum proteins VI The extension of the standard biuret method to the estimation of total protein in urine
15. Albumin standards and the measurement of serum albumin with bromcresol green
16. A candidate reference method for determination of total protein in serum I Development and validation
17. Determination of urinary total protein by use of gel filtration and a modified biuret method
18. Proposals for standardization of total protein assays
19. Determination of serum copper and iron in a single small sample
20. Sensitive direct assay for copper in serum
21. A colorimetric direct method for the determination of serum copper by use of TAMSMB
22. A sensitive direct colorimetric assay of serum copper using 5-Br-PSAA